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Biomedical subjects

F Kotsuji

Publications and source records attributed to F Kotsuji.

At least 73 records · Page 4Linked to original sources
PubMed1986

[The influence of weight loss on the reproductive function of the female rat: changes in the estrous cycle and hypothalamo-pituitary-ovarian function during feed restriction and subsequent refeeding period].

Twelve-week-old female SD rats were restricted to an 8 g/day feed intake for 2 months followed by feeding ad libitum for 3 weeks. Feed restriction resulted in weight loss and constant diestrous. A persistent increase in the hypothalamic GnRH content was observed during the underfed period. Serum LH and PRL levels dropped throughout the period but the initial reduction in the serum FSH level was observed on the 60th day. The reductions in the pituitary content of these hormones were observed on the 30th day. The decrease in the serum and ovarian E2 levels were detected on the 14th day. Anterior pituitary showed a significant response to GnRH + TRH during the course, and the relative increases in LH, FSH and PRL were greater than those in the pretreatment rats. In the course of refeeding, the body weight returned to the control level and regular estrous cycles were reestablished on the 15th and 21st day, respectively. Elevated hypothalamic GnRH content returned to the control level on the 16th day. The changes in the pituitary-ovarian axis were prompt rebound rises in serum FSH and PRL levels and pituitary FSH content, delayed restoration of serum and pituitary LH levels and serum and ovarian E2 levels, and a marked increase in the serum and ovarian progesterone levels on the 16th day. The relative increases in FSH and PRL after GnRH + TRH injection were smaller than those in the underfed period.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1977

A schematic approach to the work-up of amenorrhea.

Amenorrhea is a symptom having many possible causes. Since amenorrhea can result from disturbed function anywhere in the hypothalamic-pituitary-ovarian-uterine axis, a specific etiologic diagnosis must be made if treatment is to be effective. For this purpose, a diagnostic scheme for the differential diagnosis of the etiology of primary and secondary amenorrhea is proposed. This scheme includes a progestin test, a cyclic estrogen and progestin test, a luteinizing hormone-releasing hormone (LH-RH) loading test, and a gonadotropin (human menopausal gonadotropin and human chorionic gonadotropin) loading test. A specific pattern of responses to LH-RH and gonadotropins exists in patients with hypothalamic, pituitary, and ovarian amenorrheas, respectively, and the character of the response may facilitate the etiologic diagnosis of amenorrhea. The clinical usefulness and/or value of the scheme in the diagnosis and treatment of amenorrheas is discussed.

Adult↗
PubMed1975

Pituitary responsiveness to synthetic luteinizing hormone releasing hormone (LH-RH) during the menstrual cycle and in female hypogonadism.

The pituitary responsiveness to synthetic luteinizing hormone releasing hormone (LH-RH) in female patients with primary and secondary hypogonadism was tested and compared with that in normal women with regular menstrual cycles. A 200 mug dose of synthetic LH-RH was injected intravenously into 15 normal menstruating women, 8 patients with primary hypogonadism and 5 patients with secondary hypogonadism and the serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) response to LH-RH was investigated using double-antibody radioimmunoassay. In normal women, the LH response to LH-RH in the preovulatory phase was greater than that seen in the follicular (p less than 0-05) and luteal phases (p less than 0-05) of the menstrual cycle. In contrast, no significant difference in the FSH response was demonstrated among the three different phases of the cycle. The serum LH response to LH-RH in primary hypogonadism was greater than that in the follicular (p less than 0-01) and luteal phases (p less than 0-01) of the cycle. The serum FSH response in primary hypogonadism was markedly greater than that in the follicular (p less than 0-01), preovulatory (p less than 0-01) and luteal phases (p less than 0-01) of the menstrual cycle. Conversely, the LH levels after LH-RH administration in secondary hypogonadism were significantly lower than in the follicular (p less than 0-01), preovulatory (p less than 0-01) and luteal phases (p less than 0-01) of the cycle. The FSH response in secondary hypogonadism was slightly below that in the follicular phase (p less than 0-05). Thus, the FSH response to LH-RH in primary hypogonadism and the LH response in secondary hypogonadism seemed to be sufficiently characteristic to have diagnostic value.

Adolescent↗
PubMed1975

Pituitary response to a new analog of luteinizing hormone releasing factor during the menstrual cycle.

A new analog of luteinizing-hormone-releasing factor, (Des-Gly-NH-2-10, Pro-ethylamide-9)-LHRF, was tested in healthy women with regular menstrual cycles. This synthetic nonapeptide amide was injected intravenously in a dose of 200 mug into 15 normal female volunteers in the follicular, preovulatory, and luteal phases of the menstrual cycle, and the serum LH and FSH responses were compared with those to the same dose of the synthetic decapeptide LHRF in 15 controls. The new analog exhibited significantly higher activity than the synthetic decapeptide LHRF in the release of LH in the follicular (P smaller than 0.05), preovulatory (P smaller than 0.01), and luteal phases (P smaller than 0.05). In contrast, no statistical significance in the magnitude of FSH response was demonstrated in any phase of the menstrual cycle. The serum LH and FSH response to the nonapeptide amide suggested a longer physiologic half-life of the new LHRF analog. The results are similar to a previous report in men with normal gonadal function, and a clinical usefulness for the new analog is expected.

Adult↗
PubMed1975

Follicle-stimulating hormone response to luteinizing-hormone-releasing hormone in patients with hydatidiform mole.

The serum concentration of immunoreactive follicle-stimulating hormone (FSH) and FSH response to synthetic luteinizing-hormone-releasing hormone (LHRH) were investigated in 9 patients with intact hydatidiform moles and 3 patients with aborted moles. The serum concentration of FSH before and after intravenous administration of 200 mug of synthetic LHRH was measured by double antibody radioimmunoassay. Serum FSH was detectable but relatively low compared with the levels seen in the follicular phase of the normal menstrual cycle in all 12 patients. Furthermore, the 12 patients did not respond, or responded subnormally, to exogenously administered LHRH, and there was no significant elevation in serum FSH levels after LHRH injection. The result of the current study is in accord with that of our previous report on the serum concentration of FSH and FSH response to synthetic LHRH during normal pregnancy. Thus, it might be concluded that the follicle-stimulating activity of the anterior pituitary is suppressed by an unknown mechanism during molar pregnancy as well as normal pregnancy.

Adult↗