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Biomedical subjects

F Krombach

Publications and source records attributed to F Krombach.

At least 73 records · Page 4Linked to original sources
PubMed1990

The effect of gamma-interferon on HLA class II antigen expression on isolated human nasal chondrocytes.

HLA class II antigens play an important role in the immunological response. In this study, we report on HLA class II antigen expression in vitro by human nasal cartilage cells as detected with an immunoperoxidase staining and immunofluorescence flow cytometric analysis using monoclonal antibodies. Their expression was induced during a 7-day incubation period with gamma-interferon. These findings suggest that a possible mode of action of the preservation methods of cartilage allografts for inducing a prolonged acceptance time is based on the prevention of HLA class II antigen expression by the cartilage cells.

Antibodies, Monoclonal↗
PubMed1990

Proliferation rates of lymphocytes in subcutaneously transposed spleen and peripheral blood after heterotopic heart transplantation in rats.

In a transplantation model using Dark Agouti rats as heart donors and Lewis rats as recipients, mean graft survival time was 7.1 days in GII, receiving no immunosuppression, and 31.4 days in GIII, animals immunosuppressed by DSG. A higher percentage of Lb in the spleen than in the PB in the transplanted groups was detected on certain days. HCT sharply decreased in immunosuppressed animals, thus suggesting a reversible suppression of erythropoesis induced by DSG.

Animals↗
PubMed1989

Effect of thyrotropin-releasing hormone (TRH) on the chemiluminescence (CL) activity of human mononuclear cells.

The effect of the tripeptide TRH on the chemiluminescence activity of human monocytes was tested. TRH significantly (p less than 0.002) inhibited monocytes activity at pharmacological concentrations of 2 x 10(-4) M/L. The effect was not mediated via Ia or C3a-receptors as was proven by the preincubation of monocytes with the monoclonal antibodies OKIa1 and anti-C3a. Heat inactivation abolished the effect of TRH. This study has demonstrated for the first time, that pharmacological doses of TRH which had already been tentatively employed as a therapeutic agent in men can modulate immunocompetent cells.

Hot Temperature↗
PubMed1988

[Quantitative CT studies of the lung in an animal experiment model of silicosis].

High resolution, narrow section CT examinations of the lungs were carried out on 21 Javanese macaques (five controls) shortly before the conclusion of 27 months exposure to quartz and/or high pressure. The aim was to establish morphometric CT data for differentiating and quantifying normal and abnormal findings. The results show that the technique has a high sensitivity for demonstrating the pathological lung changes. Final validation depends upon the use of respiratory physiology and biochemical, cytological, and histomorphometric data.

Animals↗
PubMed1986

Peripheral blood and intrarenal phagocytic chemiluminescence during acute kidney graft rejection.

During organ graft rejection, soluble mediators of inflammation are released into the polymorphs (PMNs) and monocytes recruited from the blood. One functional capacity of polymorphs and monocytes/macrophages is the production of cytotoxic activated oxygen species upon stimulation, which may contribute to the rejection process. Nothing is known about the influence of allograft rejection on this inflammatory cell property. Chemiluminescence (CL) allows measurement of respiratory burst capacity in small cell samples. Zymosan-induced and luminol-amplified CL of diluted whole blood, separated PMNs, and mononuclear cells from peripheral venous blood, as well as of intragraft phagocytes was measured after allogeneic and autologous kidney transplantation in untreated dogs. CL of separated PMNs, mononuclear cells, and intragraft phagocytes was significantly elevated during allograft rejection. In autologous kidneys transplanted to recipients of allografts, CL was also increased in the autologous grafts during rejection of the allogeneic ones, indicating a systemic alteration in phagocyte function.

Animals↗
PubMed1985

Monitoring immunocompetent cells in the peripheral blood of stomach cancer patients after splenectomy and gastrectomy.

The status of lymphocyte subpopulations in splenectomized (Sx) stomach cancer patients (SCa-patients), assessed by monoclonal antibodies, has not been evaluated so far. Therefore subsets of peripheral white blood cells were monitored prior to and following surgical treatment in gastrectomized (Gx) and Sx (n = 64) as well as in non-Sx patients (n = 39). Postoperative surgical complications were more frequent in the Sx group. However, the 2-year survival rate of this group was higher than in non-Sx patients. Lymphocytes were significantly decreased in both groups of patients during the early postoperative period. Monocytes and polymorphonuclear cells (PMN) increased correspondingly. A significant loss of lymphocytes and their subsets in Sx patients was observed during the 1st-3rd postoperative days as compared to the Gx only patients. The OKT4/OKT8 ratios did not differ in either group of patients, whereas the OKT3+anti-B-Ly2 ratio was significantly increased in Sx patients 1 to 3 days postoperatively.

Antibodies, Monoclonal↗
PubMed1985

Palatability and digestibility of soya- and milk proteins in Callithricidae.

4 protein sources were tested for palatability, digestibility, influence on bodyweight and consistency of faeces in Callithrix jacchus and Saguinus fuscicollis. The protein sources were incorporated at crude protein levels of 12, 17 and 22% in a pelleted diet. A banana supplement was also fed which reduced the total protein content to 10.7, 14.7 and 18.3%. The test diets, fed for 3 weeks, were well accepted with the milk proteins being slightly preferred. Consumption of the 12% protein pellets was greater than their 22% equivalents. True protein digestibility was calculated to be 97% for lactalbumin, 96% for casein, 90% for soy protein concentrate and 83% for soyabean meal. Changes in bodyweight were negligible. Dry matter of faeces varied between 25% (lactalbumin diets) and 28% (casein diets), but did not change with increasing protein intake. Increasing levels of cellulose, from 2.4 to 7.2% total crude fibre lowered the apparent protein digestibility from 84.3 to 79.2% and the digestibility of dry matter from 82.2 to 76.5%.

Animals↗