Biomedical subjects

F Ling

Publications and source records attributed to F Ling.

At least 19 recordsLinked to original sources

PubMed2006

Comparative trial of the canine parvovirus, canine distemper virus and canine adenovirus type 2 fractions of two commercially available modified live vaccines.

The results of vaccinating two groups of puppies with commercial vaccines, both of which claimed to provide adequate protection with a final vaccination at 10 weeks of age, were compared. Groups of 19 and 20 puppies with similar titres of maternally derived antibodies against canine parvovirus (cpv), canine distemper virus (cdv) and canine adenovirus type 2 (cav-2) at four weeks of age were vaccinated at six and 10 weeks of age and their responses to each vaccination were measured by comparing the titres against cpv, cdv and cav-2 in the serum samples taken immediately before the vaccination and four weeks later. After the vaccination at six weeks of age, all 19 of the puppies in group 1 had responded to cpv and cdv, and 14 had responded to cav-2; in group 2, 17 of the 20 had responded to cpv, 19 to cdv and 15 to cav-2. In both groups the puppies that did not respond to the first vaccination had responded serologically to cpv, cdv and cav-2 at 10 weeks of age.

Adenoviridae Infections↗

PubMed2001

[Classification of intracranial dural arteriovenous fistula and its clinical signification].

OBJECTIVE: To develop a practical imaging calssification of intracranial dural arteriovenous fistula (DAVF) based on imaging and its clinical significance. METHOD: The lesion positions and drainage patterns of vein in 110 patients with 121 dural arteriovenous fistulas hospitalized in Xuanwu Hospital were analyzed and a new type of classification of DAVF was developed. RESULTS: The dural arteriovenous fistulas in 110 patients were classified accrding to lesion position into DAVF of dural sinus, cavernous sinus, tentorial incisure, venous plexus at skull base, and cerebral falx; or classified according to the pattern of venous drainiage into type I (draining directly into dural vein or dural sinus), type II (directly draining into dural sinus with retrograde venous drainage into cortical vein or spinal vcein), and type III (directly draining into cortical vein or spinal vein). Then, based on a combination of these two criteria, DAVF was claassified into cavernius sinus area type I (38 cases, 69.1%) and type II (17 cases, 30.9%); dural sinus area type I (11 cases, 33.3%), type II (16 cases, 56.4%), and type III (2 cases, 10.3%); and tentorial area (20 cases), skull base vein plexus area (6 cases), and cerebral falx area (1 case), all in type III. CONCLUSION: The newly developed classification system of intracranial dural arteriovenous fistula helps analyze clinical risk and determine therapeutic procedures.

Adolescent↗

PubMed2001

Isolation and purification of the ovulation-inducing factor from seminal plasma in the bactrian camel (Camelus bactrianus).

The purpose of this study was to extract, identify and partially characterize a newly found ovulation-inducing factor, and thus gain our understanding of induced ovulation in biology. In our preliminary research, an ovulation-inducing factor (OIF) was isolated and purified from seminal plasma of the bactrian camel by ion-exchange chromatography on DEAE-cellulose, HPLC and reverse-phase HPLC. The OIF is a peptide with 74 residues and GnRH-like bioactivity, which is heat-stable in camel seminal plasma because the OIF is wrapped up in several protein layers with different properties. However, purified OIF is degraded when it is exposed to oxygen or is heated in water. According to the analysis of amino acid components and partial amino acid sequence aminated N-terminus, and its molecular weight, the OIF is completely different from the native-LHRH, LH, HCG, PMSG and PGF-2alpha. The OIF is a novel ovulation hormone in the bactrian camel, but is similar to that reported in the bull.

Amino Acid Sequence↗

PubMed2001

[3-D angiography in diagnosis and treatment of intracranial aneurysm].

OBJECTIVE: To summarize the results of initial application of three-dimensional angiography (3D-Angio) in diagnosis and treatment of intracranial aneurysms. METHODS: From August 1999 to January 2001, standard digital subtraction angiography (two-dimensional angiography, 2D-Angio) and 3D-Angio(obtained by reconstruction of rotational angiography) were performed in 65 patients with confirmed or suspected aneurysms. RESULTS: Sixty-six aneurysms were found in 60 patients and nothing was found in 5. Of 66 the aneurysms (60 cases), 46 (43 cases) were treated by intrasaccule embolizotion with coils, 3 (3 cases) by occluding the parent artery with detachable balloon, 10 (10 cases) by surgery, and 1 (1 case) by combination of extracranial-intracranial bypass and balloon occlusion of parent artery. Six (3 cases) were not treated. Two aneurysms that could not be detected by 2D-Angio were diagnosed by 3D-Angio. Three aneurysms that were difficult or dangerous to embolize by 2D-Angio were embolized successfully with the help of 3D-Angio. Three aneurysms seemed to be embolized on 2D-Angio were proved impossible to be embolized on 3D-Angio. Of the 46 aneurysms embolized intrasaccularly, 38(82.6%) were completely embolized on 2D-Angio. In the 38, 8 had residual aneurysm on 3D-Angio. CONCLUSION: More accurate diagnosis and secure treatment of intracranial aneurysm can be reached by 3D-Angio.

Adolescent↗

PubMed2001

[Treatment of cerebral dural arteriovenous fistula targetting drainage vein or sinus].

OBJECTIVE: To investigate the efficacy of occluding the drainage veins or sinus in patients with cerebral dural arteriovenous fistulae. METHODS: Twenty patients with cerebral dural arteriovenous fistulae involving cavernous sinus (8), transverse-sigmoid sinus (6), tentorial sinus (3), superior sagittal (1), Galen (1) and straight sinus (1), were treated by transvenous embolization (14 patients), surgical interruption of venous drainage (5) and sinus isolation (1) respectively. RESULTS: Clinical cure was obtained in 13 patients, symptoms were relieved in 6 and aggravated in one. Angiographically, complete cure was noted in 11 patients, partial disappearance of fistulas in 9. Sixteen patients were followed up for 1-48 months. CONCLUSION: Targetting drainage vein or sinus occlusion is effective and safe in the management of patients with cerebral dural arteriovenous fistulae.

Adult↗

PubMed2000

A role for MHR1, a gene required for mitochondrial genetic recombination, in the repair of damage spontaneously introduced in yeast mtDNA.

A nuclear recessive mutant in Saccharomyces cerevisiae, mhr1-1, is defective in mitochondrial genetic recombination at 30 degrees C and shows extensive vegetative petite induction by UV irradiation at 30 degrees C or when cultivated at a higher temperature (37 degrees C). It has been postulated that mitochondrial DNA (mtDNA) is oxidatively damaged by by-products of oxidative respiration. Since genetic recombination plays a critical role in DNA repair in various organisms, we tested the possibility that MHR1 plays a role in the repair of oxidatively damaged mtDNA using an enzyme assay. mtDNA isolated from cells grown under standard (aerobic) conditions contained a much higher level of DNA lesions compared with mtDNA isolated from anaerobically grown cells. Soon after a temperature shift from 30 to 37 degrees C the number of mtDNA lesions increased 2-fold in mhr1-1 mutant cells but not in MHR1 cells. Malonic acid, which decreased the oxidative stress in mitochondria, partially suppressed both petite induction and the temperature-induced increase in the amount of mtDNA damage in mhr1-1 cells at 37 degrees C. Thus, functional mitochondria require active MHR1, which keeps the extent of spontaneous oxidative damage in mtDNA within a tolerable level. These observations are consistent with MHR1 having a possible role in mtDNA repair.

Aerobiosis↗

PubMed2000

Tubulocystic ovarian clear cell carcinoma with abundant fibrous stroma: malignant clear cell adenofibroma.

We report a case of tubulocystic ovarian clear cell carcinoma (OCCC) with abundant fibrous stroma associated with an endometriotic cyst. Most OCCC show a small amount of fibrous stroma; however, the tumor presented in this case had abundant stroma, that qualifies it as a malignant clear cell adenofibroma. This unusual type of clear cell carcinoma may be misinterpreted as a benign lesion or as metastatic carcinoma on frozen section. In permanent sections, the stromal invasive foci are focal, small and subtle. Therefore, extensive sampling of the specimen to search for evidence of invasion is recommended for a fibrous ovarian tumor that appears benign on gross examination.

Adenofibroma↗

PubMed2000

[An occlusive evaluation proposal to intra-saccular embolization of intracranial aneurysm].

OBJECTIVE: To explore the imaging criteria for evaluating intracranial aneurysm embolization. METHODS: Angiographic occlusive criteria for intracranial aneurysm embolization (aneurysm not opcified angiographically after embolization, 100% occlusion; a little part of aneurysm neck residual, 95%; neck residual, 90%; neck and a little part of aneurysm cavity residual, 80%; some cavity residual, < 80%) were used by 6 specialists to evaluate 121 aneurysms embolized with MDS (mechanical detachable spiral) and/or GDC (guglielmi detachable coil) from March 1995 to July 1999. The rationality, feasibility and limitation of the criteria were discussed. RESULTS: Among the 121 aneurysms, 100% occlusion was reached in 53 aneurysms (43.8%), 95% in 27 (22.3%), 90% in 16 (13.2%), 80% in 15 (12.4%), and less than 80% in 10 (8.3%) respectively. Good accordance was obtained among different doctors in the occlusive evaluation of aneurysm embolization. CONCLUSIONS: The suggesting criteria are simple and feasible in clinical practice, although the effect of imaging follow-up of embolized aneurysm is unknown and the numerical value of occlusive percentage is arbitrary.

Adult↗

PubMed1999

A mutation in a mitochondrial ABC transporter results in mitochondrial dysfunction through oxidative damage of mitochondrial DNA.

We have isolated a Saccharomyces cerevisiae mutant that shows an increased tendency to form cytoplasmic petites (respiration-deficient rho- or rho0 mutants) in response to treatment of cells growing on a solid medium with the DNA-damaging agent methyl methane-sulfonate or ultraviolet light. The mutation in this strain, atm1-1, was found to cause a single amino acid substitution in ATM1, a nuclear gene that encodes the mitochondrial ATP-binding cassette (ABC) transporter. When the mutant cells were grown in liquid glucose medium, they accumulated free iron within the mitochondria and at the same time gave rise to spontaneous cytoplasmic petite mutants, as seen previously in cells carrying a mutation in a gene homologous to the human gene responsible for Friedreich's ataxia. Analysis of the effects of free iron and malonic acid (an inhibitor of oxidative respiration in mitochondria) on the incidence of petites among the mutant cells indicated that spontaneous induction of petites was a consequence of oxidative stress rather than a direct effect of either a defect in the ATM1 gene or the accumulation of free iron. We observed an increase in the incidence of strand breaks in the mitochondrial DNA of the atm1-1 mutant cells. Furthermore, we found that rates of induction of petites and accumulation of strand breaks in mitochondrial DNA were enhanced in the atm1-1 mutant by the introduction of another mutation, mhr1-1, which results in a deficiency in mitochondrial DNA repair. These observations indicate that spontaneous induction of petites in the atm1-1 mutant is a consequence of oxidative damage to mitochondrial DNA mediated by enhanced accumulation of mitochondrial iron.

ATP-Binding Cassette Transporters↗

PubMed1999

Is premenstrual dysphoric disorder a distinct clinical entity?

Does the evidence now available support the concept of premenstrual dysphoric disorder (PMDD) as a distinct clinical disorder such that the relative safety and efficacy of potential treatment can be evaluated? In a roundtable discussion of this question, a wealth of information was reviewed by a panel of experts. The key characteristics of PMDD, with clear onset and offset of symptoms closely linked to the menstrual cycle and the prominence of symptoms of anger, irritability, and internal tension, were contrasted with those of known mood and anxiety disorders. PMDD displays a distinct clinical picture that, in the absence of treatment, is remarkably stable from cycle to cycle and over time. Effective treatment of PMDD can be accomplished with serotinergic agents. At least 60% of patients respond to selective serotonin reuptake inhibitors (SSRIs). In comparison with other disorders, PMDD symptoms respond to low doses of SSRIs and to intermittent dosing. Normal functioning of the hypothalamic-pituitary-adrenal (HPA) axis, biologic characteristics generally related to the serotonin system, and a genetic component unrelated to major depression are further features of PMDD that separate it from other affective (mood) disorders. Based on this evidence, the consensus of the group was that PMDD is a distinct clinical entity. Potential treatments for this disorder can now be evaluated on this basis to meet the clear need for effective therapy.

Comorbidity↗

PubMed1999

[Selective arterial embolization for the treatment of thoracolumbar spinal tumor].

OBJECTIVE: To decrease intraoperative blood loss during tumor resection. METHODS: Fifteen patients with primary thoraco-lumbar spinal tumor [giant cell tumor (5 patients), malignant nerinoma (2), chordoma (1), fibrous xanthosarcoma (1), malignant fibrohistocytoma (1), osteosarcoma (1), Ewing sarcoma (1), myeloma (1), leimyosarcoma (1), Non-Hodgkin diseases (1)] were treated by means of preoperative selective arterial embolization, tumor resection, and spinal reconstruction. Eight patients were subjected to total spondylectomy in one stage. RESULTS: Fourteen patients showed satisfactory results after embolization. The volume of intraoperative blood loss ranged from 3,000 ml to 400 ml (average 1,200 ml). Follow up of the patients varied from 8 months to 19 years (average 48.7 months). Local recurrence happened in 3 patients; 3 patients died from metastasis. Three of 4 patients recovered from complete bowel movement and urination. Seven of nine patients had musical strength improved. Six of 8 patients had recovery of the e sensation of the lower limbs. CONCLUSIONS: Selective arterial embolization before operation followed by operation within 24 hours can reduce intraoperative blood loss, shorten operative time, and provide a clear operative field for tumor resection.

Adolescent↗

PubMed1999

[Refractory carotid-cavernous fistula: causes and countermeasures].

OBJECTIVE: To explore the causes and countermeasures of refractory carotid-cavernous fistula (CCF). METHODS: Twelve refractory cases from 123 cases of consecutive traumatic CCF during 12 years were reviewed. RESULTS: The main causes of refractory CCF were: small or large fistula's opening, or constrictive parent artery; inappropriate early treatments such as ligature of internal carotid artery or common carotid artery, balloon detachment of, and non-dense packing of coils inside the cavernous sinus; unexpected deflation or balloon displacement of resulting in fistula recurrence. Anatomical cure was achieved in 11 cases, and clinical care in 1 by using balloon or/and coil or/and NBCA (n-butal 2-cyanoacrylate) through arterial, venous or surgical approach. CONCLUSIONS: refractory CCF can be treated effectively skilled catheterization and embolization as well as appropriate approach and embolic material according to fistula structure and vascular route.

Adolescent↗

PubMed1998

Glucose repression on RIM1, a gene encoding a mitochondrial single-stranded DNA-binding protein, in Saccharomyces cerevisiae: a possible regulation at pre-mRNA splicing.

The mitochondrial single-stranded DNA-binding protein (SSB) encoded by anuclear gene, RIM1, is a homolog of Escherichia coli SSB. The addition of glucose decreased the amount of RIM1-mRNA in cells growing in a glycerol medium, but increased the amount of the immature RIM1-mRNA. The changes in the amounts of both mature and immature RIM1-mRNAs were dependent on SRN1/REG1/HEX2, a gene relating to pre-mRNA-splicing and glucose repression. These observations suggest that the expression of the mitochondrial SSB is regulated, at least in part, by pre-mRNA splicing under the control of glucose repression.

Culture Media↗

PubMed1998

[Endovascular treatment of intracranial aneurysms with GDC: report of 8 cases].

OBJECTIVE: To assess the usefulness and practical experience in intracranial aneurysm embolization with guglielmi detachable coil (GDC) applied firstly in China. METHOD: Under general anesthesia and systematic anticoagulation, eight cases of intracranial aneurysms were embolized with GDC which was introduced by tracker micro-catheter, combined remodeling technique (RT) if necessary. RESULT: 100% occlusion was achieved in 5 cases, 95% in 2 and 90% in 1, without any complication. Among these cases, 4 failed in the previous attempts of endovascular treatment with mechanical detachable system (MDS), because MDS could not be safely and totally placed inside the aneurysms and Mag 3F/2F micro-catheter could not be navigated into the aneurysms. CONCLUSION: Embolization of intracranial aneurysms with GDC is safe, effective and reliable, and it may offer the cure opportunity to those enearysms which are very dangerous to embolize or can not be embolized with MDS.

Adult↗

PubMed1998

[Animal experiment and clinical application of self-made mechanically detachable coils].

OBJECTIVE: To evaluate the practical and thrombogenetic characteristics of self-made mechanically detachable coils by the animal experiment and primary clinical application. METHOD: The bifurcation type of aneurysm model was established on rabbit and embolized with self-made mechanically detachable coils 2 weeks after surgery. 24 coils were used in 5 experimental aneurysms. Control angiography was performed and the rabbits were sacrificed 2 - 3 weeks after the embolization. The embolized aneurysm samples were examined by pathologist. Ten patients were treated with self-made mechanically detachable coils in primary clinical application. They were divided into aneurysms (7 patients), traumatic carotid-carvenous fistulas (2), dural arterio-venous fistula in carvenous sinus region (1). 146 coils were used totally and all were delivered with mechanical detachable system (MDS Balt co. France). RESULT: Pathologically, the cavities of rabbit aneurysm models were occluded by tungsten coils (2 complete, 3 almost complete). The coils were trapped in granulation tissue which was covered by a thin layer of endothelium on the surface facing the blood flow. In the coils dense packing ones, the aneurysms were completely excluded from the parent circulation by neointimal layers. In the practice procedures, the coils fit MDS (Balt co. France) very well and can be delivered, withdrew and detached smoothly. CONCLUSION: The self-made mechanically detachable coils have practical reliability and evident thrombogenetic effects.

Adult↗

PubMed1998

[Interventional endovascular therapeutic modalities of giant intracranial aneurysm].

OBJECTIVE: To treat 28 cases of intracranial giant aneurysms in the last 7 years and study the therapeutic methods and their effectiveness on this disease. METHOD: The cases of giant aneurysms were divided into several groups. The advantages and disadvantages of the methods were compared. The main modality of endovascular therapy was to use balloon to occlude the parent artery. RESULT: In the 16 cases treated by the proximal balloon occlusion, 13 were cured angiographically with complete obliteration of the aneurysmal sac and in 4 of them the aneurysms disappeared both on CT and MRI. No deterioration after operation was encountered with the exception of 2 deaths. CONCLUSION: Endovascular therapy can cure the intracranial aneurysms which were difficulty treated by direct open operation.

Adolescent↗

PubMed1998

[The preliminary analysis of domestic hydrogel microspheres for endovascular embolization].

OBJECTIVE: To evaluate the embolic effects and biocompatibility of domestic hydrogel microspheres (HMs). METHOD: 22 domestic rabbits were used. 0.03 g/2ml, 0.06 g/2ml domestic Hms of 70 - 150 microm and 2 ml normal saline were injected respectively through catheterization in the right common carotid arteries of rabbits. The changes in neurologic functions, general state and average weight of rabbits were observed, and the histomorphological changes of embolized arteries and surrounding tissues were evaluated by analysis of macroscopy and light microscope. RESULT: Rabbits' neurologic symptoms (hemiparalysis, convulsion and hypotonia) and death were observed after embolization. Thrombogenesis was present and the entire cross sections of blood vessels could easily be embolized. Neither acute and chronic inflammatory cells nor remarkable general toxic and side effects were shown in rabbits. CONCLUSION: Domestic HMs can effectively block the vessels, and they have the characteristics of good biocompatibility. The kind of domestic HMs is a promising particulate embolic agent in clinical sets.

Animals↗

PubMed1998

[Surgical treatment of spinal dural arteriovenous fistulas].

OBJECTIVE: To discuss the operation modalities of spinal dural arteriovenous fistulae (SDAVFs) and treatment with anticoagulant agents after surgery. METHOD: The medical records, radiographic studies and operations of 23 patients with SDAVFs were reviewed. Laminectomy was performed at the level of the arterialized venous entry into the subarachnoid space in 18 patients with thoracic and lumbar lever AVFs, followed by coagulation, clipping and division of the vein. In 14 of 18 patients, the arteriovenous nidus in the dura was identified and obliterated by coagulation. The draining veins were interrupted at L(5) - S(1) level in 5 patients with sacral AVFs. Anticoagulant treatment was given in 13 patients. RESULT: Outcome was good in 20 patients and 2 of the 20 had transient postoperative neurological deterioration that reversed by anticoagulant agents. No changes were seen in 2 patients except one deteriorated. CONCLUSION: Surgical interruption of arterialized radicular-medullary draining vein of SDAVFs provides lasting occlusion of the fistula, and postoperative anticoagulant therapy can prevent thrombosis of the coronal venous plexus.

Adult↗