Biomedical subjects

F Ménissier

Publications and source records attributed to F Ménissier.

10 recordsLinked to original sources

PubMed2000

Age-related changes and location of types I, III, XII and XIV collagen during development of skeletal muscles from genetically different animals.

The ontogenesis of total collagen and of different collagen types was studied in four muscle types from genetically different cattle. Hydroxyproline content was 1.2-fold higher in muscles from cross-bred foetuses with normal muscle growth compared to those of the other genetic types (pure bred with different growth rates, double-muscled breed). A similar tendency was observed for type III collagen content. In all muscles of each animal studied, type XII and XIV collagens were colocated in perimysium. Immunolabelling obtained for type XII collagen was higher during foetal life than after birth, while for type XIV collagen, the opposite result was obtained. Whatever the muscle studied, but especially in semitendinosus muscle, during the foetal and the post-natal period until 15 months of age, immunolabelling with antibody anti-type XIV collagen tended to be more intense in muscles of animals from fathers selected for a low muscle growth capacity compared to those from fathers selected for a high muscle growth capacity. In conclusion, this study shows, that during foetal life, selection according to muscle growth capacity has no significant effect on the contents of total hydroxyproline or type III collagen, but minor effects on collagen localization.

Age Factors↗

PubMed1998

Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle.

We have determined the entire myostatin coding sequence for 32 double-muscled cattle sampled from ten European cattle breeds. Seven DNA sequence polymorphisms were identified, of which five would be predicted to disrupt the function of the protein, one is a conservative amino acid substitution, and one a silent DNA sequence variant. Four additional DNA sequence polymorphisms were identified in myostatin intronic sequences. In all but two breeds, all double-muscled animals were either homozygous or compound heterozygotes for one of the five loss-of-function mutations. The absence of obvious loss-of-function mutations in the coding sequence of the two remaining breeds points either towards additional mutations in unexplored segments of the gene, or towards locus heterogeneity of double-muscling.

Alleles↗

PubMed1997

A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle.

An exceptional muscle development commonly referred to as 'double-muscled' (Fig. 1) has been seen in several cattle breeds and has attracted considerable attention from beef producers. Double-muscled animals are characterized by an increase in muscle mass of about 20%, due to general skeletal-muscle hyperplasia-that is, an increase in the number of muscle fibers rather than in their individual diameter. Although the hereditary nature of the double-muscled condition was recognized early on, the precise mode of inheritance has remained controversial; monogenic (domainant and recessive), oligogenic and polygenic models have been proposed. In the Belgian Blue cattle breed (BBCB), segregation analysis performed both in experimental crosses and in the outbred population suggested an autosomal recessive inheritance. This was confirmed when the muscular hypertrophy (mh) locus was mapped 3.1 cM from microsatellite TGLA44 on the centromeric end of bovine chromosome 2 (ref. 5). We used a positional candidate approach to demonstrate that a mutation in bovine MSTN, which encodes myostatin, a member of the TGF beta superfamily, is responsible for the double-muscled phenotype. We report an 11-bp deletion in the coding sequence for the bioactive carboxy-terminal domain of the protein causing the muscular hypertrophy observed in Belgian Blue cattle.

Amino Acid Sequence↗

PubMed1995

Individual differences in docility in Limousin cattle.

Docility tests were performed over 3 yr on 906 Limousin heifers sired by 34 males. The heifers were tested individually when they were 10 to 11 mo old in a pen with a handler who had 2 min to lead the animal to a corner, keep it there for 30 consecutive seconds, and then to stroke it. Behaviours of the animals were recorded and scores calculated. The first score (docility score) was a continuous variable combining the greatest possible information. The second score (docility criterion) was a categorical trait with four classes. Tests were performed by seven different handlers. The effects of handler, year, birth period, dam parity, birth region, husbandry system, and sire were estimated. Handlers, birth period, husbandry system, and sire had significant effects. In particular, animals that were managed indoors were more docile than those reared out-of-doors at least part of the time. Heritability estimates of the docility score (.22) and docility criterion (.18) seem sufficient to select for docility traits.

Aggression↗

PubMed1994

Detection of pregnancy by radioimmunoassay of a pregnancy serum protein (PSP60) in cattle.

The accuracy and efficiency of pregnancy diagnoses in cattle by pregnancy serum protein (PSP60) radioimmunoassay, a progesterone radioimmunoassay or oestrus detection were compared. Blood samples were taken from 349 suckling heifers and cows (1,191 inseminations) at 28, 35, 50 and 90 d post-insemination for PSP60 determination and at 22-23 d for progesterone. Females were declared nonpregnant when plasma PSP60 concentration was lower than 0.2 ng/ml at 28, 35 and 50 d and 0.5 ng/ml at 90 d. When compared with rectal palpation at 90 d, the accuracy of positive (negative) diagnoses by progesterone assay was 80% (100%) in heifers and 75% (99%) in cows. The accuracy of positive diagnoses by PSP60 assay increased with gestation stage from 90% on d 28 in heifers (74% in cows) to 100% (99% in cows) at the time of rectal palpation. This accuracy was 84% on d 28 in cows when the interval from calving to blood sampling was higher than 115 d. Whatever the stage, the accuracy of negative diagnoses was higher than 90%. Efficiency in detecting pregnant or nonpregnant females on d 28 was equivalent to the progesterone assay. The method for detecting oestrus applied in this experiment was as efficient as the PSP60 or progesterone test at any stage of gestation. The PSP60 test is very flexible, which makes its use particularly interesting in naturally mated suckling herds because of the uncertainty regarding the date of fertilization.

Animals↗

PubMed1993

Peripheral concentrations of a 60-kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle.

In order to have a specific marker for studying pregnancy in cattle we examined the characteristics of a pregnancy serum protein produced by the placenta (PSP60). Its profile in peripheral blood was determined by radioimmunoassay in pregnant cows of 3 breeds after artificial insemination (AI): Charolais (n = 24), Normande (n = 24) and Holstein (n = 26). From 27 d post-AI to the end of pregnancy the plasma PSP60 concentration increased, especially during the last 2 wk, to reach a peak a few d before calving, which was higher (P < 0.001) in the Charolais (1,238 +/- 422 ng/ml) than in the other breeds (528 +/- 458 and 444 +/- 204 ng/ml). With an apparent half-life of approximately 8 d, this protein was still detectable in the maternal blood from 105, 85 and 87 d post-partum in the Charolais, Normande and Holstein breeds, respectively. Fertility results on 1,102 inseminations in the Charolais breed showed that the PSP60 concentration dropped then disappeared after embryonic mortality. Sequential assays of this protein between 28-90 d after AI are useful for studying the course of pregnancy, although they do not allow discrimination between early embryonic mortality and non-fertilization which together constitute 75% of pregnancy failures.

Animals↗

PubMedDate not supplied

Genetic variability of the length of postpartum anoestrus in Charolais cows and its relationship with age at puberty.

Fertility records (n = 1,802) were collected from 615 Charolais primiparous and multiparous cows managed in an experimental herd over an 11-year period. The objectives of the study were to describe the genetic variability of the re-establishment of postpartum reproductive activity and the relationship with body weight (BW) and body condition score (BCS) at calving and age at puberty. The length of postpartum anoestrus was estimated based on weekly blood progesterone assays and on twice daily detection of oestrus behaviour. The first oestrus behaviour was observed 69 days (+/-25 days s.d.) post-calving and the first positive progesterone measurement (>or=1ng x ml(-1)) was observed at 66 days (+/-22 days s.d.) for the group of easy-calving multiparous suckling cows. Estimates of heritability and repeatability were h2 = 0.12 and r = 0.38 respectively, for the interval from calving to first oestrus (ICO). Corresponding values were h2 = 0.35 and r = 0.60 for the interval from calving to the first positive progesterone test (ICP). The genetic correlation between both criteria was high (rg = 0.98). The genetic relationships between postpartum intervals and BW and BCS of the female at calving were negative: the genetic aptitude to be heavier at calving and to have high body reserves was related to shorter postpartum intervals. A favourable genetic correlation between age at puberty and postpartum intervals was found (rg between 0.45 and 0.70). The heifers which were genetically younger at puberty also had shorter postpartum intervals.

Journal Article↗

PubMedDate not supplied

Genetic correlation estimations between artificial insemination sire performances and their progeny beef traits both measured in test stations.

In France, beef traits of artificial insemination (AI) beef bulls are improved through the sequential selection for their own performances and for their male progeny performances, both being recorded in test stations. The efficiency of such programmes mainly depends on the genetic correlations between sire performances and progeny beef traits. Such correlations were independently estimated, using the multivariate REML (restricted maximum likelihood) method in a Limousin and a Charolais programme. In both breeds, high genetic correlations were observed between sires and progeny analogous morphology scores (from 0.64 to 0.82). Genetic correlations estimated between sires and progeny growth (from 0.41 to 0.70) were lower probably due to the difference of diet in central and progeny stations. Correlations between sire muscling scores and progeny skeletal frames (and vice-versa) were negative (from -0.05 to -0.58). The genetic correlations of sire traits with progeny dressing percentage (DPp) and carcass fatness score (CFp) were only low to moderate. These results show that the selection of bulls at the end of performance testing in test stations may be efficient in improving progeny growth and morphology. However, such a selection is insufficient in improving their dressing percentage and carcass composition.

Journal Article↗

PubMedDate not supplied

A first genotyping assay of French cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (Mc1r).

The seven transmembrane domain melanocortin-1 receptor (Mc1r) encoded by the coat color extension gene (E) plays a key role in the signaling pathway of melanin synthesis. Upon the binding of agonist (melanocortin hormone, alpha-MSH) or antagonist (Agouti protein) ligands, the melanosomal synthesis of eumelanin and/or phaeomelanin pigments is stimulated or inhibited, respectively. Different alleles of the extension gene were cloned from unrelated animals belonging to French cattle breeds and sequenced. The wild type E allele was mainly present in Normande cattle, the dominant E(D) allele in animals with black color (i.e. Holstein), whereas the recessive e allele was identified in homozygous animals exhibiting a more or less strong red coat color (Blonde d'Aquitaine, Charolaise, Limousine and Salers). A new allele, named E1, was found in either homozygous (E1/E1) or heterozygous (E1/E) individuals in Aubrac and Gasconne breeds. This allele displayed a 4 amino acid duplication (12 nucleotides) located within the third cytoplasmic loop of the receptor, a region known to interact with G proteins. A first genotyping assay of the main French cattle breeds is described based on these four extension alleles.

Journal Article↗

PubMedDate not supplied

Genetic relationship between cyclic ovarian activity in heifers and cows and beef traits in males.

Records were collected in an experimental herd over an 11-year period from purebred Charolais heifers (n=351), cows (n=615) and young entire bulls (n=383). The objective of the study was to estimate the genetic relationship between the components of female ovarian activity (age at puberty and postpartum anoestrus length), their growth rate and body condition score and beef traits measured on related bulls. Two methods were used to estimate age at puberty and postpartum anoestrus length: the detection of oestrous behaviour and a test of cyclicity based on plasmatic progesterone assay. This study shows the existence of significant heritability estimates for the different cyclicity traits (h(2) between 0.11 and 0.38). Most of the genetic correlation coefficients between ovarian activity and growth rate of females and males are negative and favourable (r(g) between - 0.43 and 0.06). Cyclicity is also favourably related with body condition score in young or adult females (r(g) between - 0.65 and - 0.22). The genetic relationship between female ovarian activity and proportion of adipose tissue in the male carcass is, however, close to zero. These results show that an antagonism between male beef traits measured in this study and female ovarian activity is unlikely to be a cause for concern in the short term.

Anestrus↗