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Biomedical subjects

F Mannocci

Publications and source records attributed to F Mannocci.

At least 19 recordsLinked to original sources

The penetration of RealSeal primer and Tubliseal into root canal dentinal tubules: a confocal microscopic study.

AIM: To compare penetration depth into dentinal tubules of RealSeal with that of a well-established endodontic sealer (Tubliseal) by means of confocal microscopy. METHODOLOGY: Twenty sound extracted, single-rooted premolars were selected. Following completion of root canal instrumentation, the teeth were divided into two groups using a stratified sampling method, ranking teeth according to size. In group 1, 10 teeth were filled with Gutta-percha and Tubliseal using cold lateral condensation. In group 2, 10 teeth were filled with RealSeal. Both sealers were labelled with Rhodamine B dye. The teeth were sectioned parallel to their long axis resulting in 20 specimens per group. Confocal microscopy was used to assess the penetration depths of the sealers at three sites for each specimen (coronal, middle and apical). Data were analysed statistically using Stata Release 9.1. RESULTS: The penetration depth of RealSeal in each one of the thirds of the root canal was found to be higher than that of Tubliseal (P < 0.05). The penetration depths of the two sealers was found to be significantly different (P = 0.001). The mean penetration value for the RealSeal group was 908.8 microm whereas the mean value for the Tubliseal group was 139.5 microm. CONCLUSIONS: The penetration depth of RealSeal into the root dentinal tubules is significantly greater than that of Tubliseal.

Bicuspid↗

Hand and nickel-titanium root canal instrumentation performed by dental students: a micro-computed tomographic study.

The aim of this study was to evaluate root canals instrumented by dental students using the modified double-flared technique, nickel-titanium (NiTi) rotary System GT files and NiTi rotary ProTaper files by micro-computed tomography (MCT). A total of 36 root canals from 18 mesial roots of mandibular molar teeth were prepared; 12 canals were prepared with the modified double-flared technique, using K-flexofiles and Gates-Glidden burs; 12 canals were prepared using System GT and 12 using ProTaper rotary files. Each root was scanned using MCT preoperatively and postoperatively. At the coronal and mid-root sections, System GT and ProTaper files produced significantly less enlarged canal cross-sectional area, volume and perimeter than the modified double-flared technique (P < 0.05). In the mid-root sections there was significantly less thinning of the root structure towards the furcation with System GT and ProTaper (P < 0.05). The rotary techniques were both three times faster than the modified double-flared technique (P < 0.05). Qualitative evaluation of the preparations showed that both ProTaper and System GT were able to prepare root canals with little or no procedural error compared with the modified double-flared technique. Under the conditions of this study, inexperienced dental students were able to prepare curved root canals with rotary files with greater preservation of tooth structure, low risk of procedural errors and much quicker than with hand instruments.

Dental Alloys↗

Penetration of bonding resins into fibre-reinforced composite posts: a confocal microscopic study.

AIM: To compare the penetration of two different bonding resins applied for different contact times, on glass reinforced-reinforced composite root canal posts showing an interpenetrating polymer network (IPN) or cross-linked polymer matrix. The research hypothesis was that the bonding resins were equally able to penetrate into the IPN post whereas less penetration was evident into the cross-linked post. In addition, the prolonged contact time of the bonding resin on the surface of post was assumed to increase the resin penetration. METHODOLOGY: A total of 36 posts, comprising 18 IPN posts (everStick Post) and 18 cross-linked posts (C Post Millennium) were divided into 12 groups of three posts. All posts had a diameter of 1.2 mm. Both bonding resins (Scotchbond Multi Purpose Plus and Stick Resin) were labelled with Rhodamine B for determination of the resin penetration into the post. After contact times of 1, 30 and 300 s the bonding resins were light cured for 60 s. Penetration of bonding resins into three sections of each post was measured by confocal scanning light microscope. The influences of post and type of adhesive resin on resin diffusion into the polymer matrix of the post were assessed by nonparametric methods. RESULTS: For C Post Millennium and for 1 s contact time, no penetration of bonding resins into the polymer matrix of posts occurred in any section of any post. For the everStick Post, penetration was always noted after 300 s contact (mean 22.8 microm) and nearly always after 30 s contact (mean 3.5 microm), with very little difference between the two adhesives. The degree of penetration into everStick Posts was invariably greater after 300 s contact than after 30 s (P < 0.001). Both sets of results were highly significantly greater than the zero penetration recorded using C Post Millennium or 1 s contact time (P < 0.001). CONCLUSIONS: The ability of bonding resins to penetrate into everStick Post with IPN polymer matrix may give the opportunity to establish a good link between fibre-reinforced composite posts, luting cements and composite cores.

Composite Resins↗

The isthmuses of the mesial root of mandibular molars: a micro-computed tomographic study.

AIM: To investigate the prevalence of root canal isthmuses in the apical 5 mm of the mesial root of mandibular molars by means of micro-computed tomography (MCT) and to describe the morphology of the isthmuses. METHODOLOGY: Twenty extracted mandibular first molars that had fully formed roots were selected. The mesial roots were sectioned from the distal roots and subjected to MCT. The number of sections showing isthmuses in each of the apical 5 mm of the root canals was recorded. In each one of the apical 5 mm of the 20 roots examined, 40 sections were observed, giving a total of 800 sections observed in each group. Data were analysed as a contingency table using the chi-square statistic to test the null hypothesis that location of the sections in each of the apical 5 mm and presence of the isthmus were independent. RESULTS: Isthmuses were found to be present at all levels with prevalence figures between 17.25 and 50.25%. The chi-square test indicated a significant difference in the distribution of isthmuses with section (P = 0.001). It was found that sections in the first millimetre from the apex had fewer isthmuses than expected and that sections in the third millimetre from the apex had more isthmuses than expected under the null hypothesis. Calcifications were found to be present in most isthmuses, occasionally lateral canals originated from the central part of the isthmuses. CONCLUSIONS: Isthmuses were present in the vast majority of roots observed. The third millimetre from the apex showed more isthmuses than expected. The results of clinical and surgical endodontic procedures performed in the mesial root of mandibular molars may be affected by this aspect of the root canal anatomy.

Dental Pulp Cavity↗

Bonding of all-porcelain crowns: structural characteristics of the substrate.

OBJECTIVES: The aim of this in vitro study was to evaluate the morphology of dentin in teeth prepared for single-unit all-porcelain crowns (SUAPC) in terms of tubule orientation, density and increase in surface area after etching. METHODS: Twenty anterior and 20 posterior teeth from adults were prepared 1mm below the cemento-enamel junction (CEJ) for SUAPC. The samples were divided into groups based on type of tooth (anterior or posterior) and bonding system employed. The teeth were processed for evaluation of morphology of the substrate, hybrid layer thickness and resin tag formation. The observations were subdivided according to location in the preparation and tubule density was calculated. RESULTS: The observations revealed the variability in tubule density and orientation in different areas within any one preparation. The morphology of the cervical margin was less predictable with the presence of cementum and an ill-defined cemento-dentinal junction. Statistically significant differences in the density of tubules were found depending on location. Groups 2, 3, 5 and 6 samples showed that the hybrid layer thickness and resin tag morphology depended on the density and direction of tubules. In those sectors with parallel and oblique tubule orientation and with a low density of tubules, the hybrid layer was significantly thinner than in areas with cross-sectioned tubules. SIGNIFICANCE: The cementum and the peripheral intertubular dentin surface area are likely to be responsible for the bond strength after acid etching of crown preparations, but not all areas exhibited equal responses to etching. In particular, the bonding substrate at the gingival margins may contribute little in terms of micromechanical retention.

Acid Etching, Dental↗

The structure of dentine in the apical region of human teeth.

AIM: To study the structure of the apical region of human teeth with emphasis on dentinal tubules and their branches. METHODOLOGY: This descriptive histological study employed demineralized stained sections for light microscopy, demineralized unstained sections for scanning electron microscopy, and undemineralized, acid-etched specimens for confocal tandem scanning microscopy. RESULTS: The apical portion of human teeth showed marked variations in structure, including accessory root canals, areas of resorption and repaired resorptions, occasional attached, embedded and free pulp stones, varied amounts of irregular secondary dentine, and even cementum-like tissue lining the apical root canal wall. The apex often deviated from the long axis of the root canal. Primary dentinal tubules were irregular in direction and density. Some areas were devoid of tubules. CONCLUSIONS: The irregular and variable structure of the apical region of human teeth represent special challenges during endodontic therapy. Obturation techniques based on the penetration of adhesives into dentinal tubules are unlikely to be successful and adhesive techniques must depend on impregnation of a hybrid layer.

Adult↗

Microleakage of endodontically treated teeth restored with fiber posts and composite cores after cyclic loading: a confocal microscopic study.

STATEMENT OF PROBLEM: The effectiveness of the seal obtained with carbon fiber posts and composite cores is still unclear. Both 3-step dental adhesives and self-etching adhesive primers have been suggested as adhesive systems. PURPOSE: This confocal microscopic study evaluated the microleakage of teeth endodontically treated and restored with fiber posts and composites with 3 adhesive systems. MATERIAL AND METHODS: A total of 72 human mandibular premolars were endodontically treated and divided into 6 groups of 12 teeth each. The first 3 groups were treated with an endodontic sealer containing zinc oxide-eugenol (ZOE) and restored with temporary filling materials containing ZOE. The last 3 groups were treated with ZOE-free materials. Post spaces were prepared in the root canals. The first group treated with ZOE-based materials was restored with fiber posts cemented with zinc phosphate cement and composite cores without adhesive. The other 2 groups of ZOE-treated teeth were restored with fiber posts cemented with All Bond 2 and Panavia 21 dental adhesives, respectively. The last 3 groups were restored with fiber posts cemented with All Bond 2, Panavia 21, and Panavia Fluoro cement, respectively. The teeth were loaded intermittently at 2 cycles per second in a moist environment and, after 300,000 cycles, immersed in a solution of Rhodamine B dye for 48 hours. A confocal microscope was used to observe the teeth. The ratio between the length of the interfaces observed and the length of the dye penetration was evaluated. Two teeth from each group acted as controls and were not subjected to dynamic loads. RESULTS: All resin cement groups leaked significantly less than the group cemented with zinc phosphate cement. No statistically significant difference was found between the microleakage of teeth treated with ZOE-based and non-ZOE-based materials. Teeth restored with All Bond 2 dental adhesive leaked significantly less than those restored with Panavia cement. CONCLUSION: The 3-step dental adhesive (All Bond 2) resulted in a better marginal seal than that obtained with the self-etching primers (Panavia 21 and Panavia F). The use of endodontic sealers and temporary filling materials containing ZOE had no detrimental effect on the marginal seal of carbon fiber post/composite resin core restorations.

Adhesives↗

Three-point bending test of fiber posts.

Five different types of fiber posts (carbon fiber Composipost radio-opaque, quartz fiber Aestheti-Plus, carbon fiber Carbotech, glass fiber Light post, and silica fiber Snowpost) were submitted to a three-point bending test after being stored for 1 yr under three different conditions: dry storage at room temperature, storage in 37 degrees C water, and storage in bovine teeth that were endodontically treated and restored with composite resin. The posts were observed before and during the test by confocal microscopy. More voids were found within the Composipost radio-opaque than in other posts. Posts of all groups that were stored in water showed flexural strength values lower than posts that were stored dry. In all storage conditions, Carbotech posts showed higher flexural strength values than Composiposts and Snowposts, and Aestheti-Plus posts showed higher flexural strength values than Snowposts. Posts that were stored in bovine teeth showed flexural strength values similar to those of posts that were stored dry. It was concluded that fiber posts must not be in contact with oral fluids and that during the 1-yr storage in bovine teeth, they were sufficiently protected from this contact by the apical root canal filling and by the coronal composite resin filling.

Animals↗

Microtensile bond strength and confocal microscopy of dental adhesives bonded to root canal dentin.

PURPOSE: To evaluate the bond strength of two dentin-bonding systems to root canal dentin. MATERIALS AND METHODS: Six central incisors, extracted for periodontal reasons, were endodontically treated, no obturation of the root canal space was performed. The teeth were cut parallel to their long axis. One half of each tooth was randomly assigned to one of two experimental groups. The teeth of the first group were treated with All-Bond 2 dental adhesive and those of the second group with Panavia F. The root canal dentin was conditioned as suggested by the manufacturers and, after the application of the dental adhesives, a layer of resin-based composite was polymerized over the adhesive layer. After the composite had completely set, the sections were prepared for microtensile test and kept in a wet environment at a relative humidity of 90 +/- 2% measured with a digital relative humidity meter and at a temperature of 22 degrees C for 24 hrs. The specimens were sectioned perpendicularly to the bonded interface, into slabs 1.5 mm thick (10 sections for each experimental group). The slabs were then trimmed by super fine diamond burs for the microtensile bond test with the narrowest region located at the respective bonded interface. The thickness of the slab was then exactly established by three measurements performed in three points of each slab by a digital micrometer. The specimens were then subjected to a tensile force at a crosshead speed of 0.2 mm/min. The test was observed using a confocal microscope and the failure modes were noted. The load at failure was registered and the bonded area was measured using a confocal microscope at a magnification of x25 in conjunction with an image-processing program. The failure data were analyzed using the Wilcoxon-Gehan test with exact non-parametric inference. RESULTS: The mean value at failure was the same for the two experimental groups (17.1 MPa). There was no statistically significant difference between Panavia F and All-Bond 2 (P> 0.8671). Most of the specimens showed a debonding of the adhesive from the hybrid layer. The results showed that the bond strength of the two bonding systems tested to the dentin of the root canal are slightly lower than to those obtained with the latest adhesive systems, in the coronal dentin.

Composite Resins↗

A 'one-bottle' adhesive system for bonding a fibre post into a root canal: an SEM evaluation of the post-resin interface.

AIM: This report presents a case in which a 'one-bottle' adhesive system was used in combination with proprietary resin cement for bonding a fibre post. METHODOLOGY: The fibre post was placed into the root canal of a fractured root under clinical conditions and then extracted 1 week later. Using scanning electron microscopy, half of the root was evaluated for hybrid layer formation and the other half for assessing resin tags. RESULTS: The investigation demonstrated that a 'one-bottle' system can infiltrate etched dentine. CONCLUSIONS: The 'one-bottle' system tested in this study can create a mechanical interlocking with root etched dentine under clinical conditions.

Acid Etching, Dental↗

Retrospective study of the clinical performance of fiber posts.

PURPOSE: To evaluate the clinical performance of C-Posts, AEstheti Posts and AEstheti Plus Posts after a period of clinical service ranging from 1-6 yrs. MATERIALS AND METHODS: 1,304 posts were included in the study: 840 Composiposts, 215 AEstheti posts and 249 AEstheti Plus posts were placed into endodontically treated teeth. Four combinations of bonding/luting materials were used. The patients were recalled every 6 months and clinical and radiographic examinations were completed. Endodontic and prosthodontic results were recorded. Actuarial Life Table statistical analysis and Mantel-Haenszel comparison of survival curve have been performed at 95% level of confidence. RESULTS: The 3.2% failure rate was due to two reasons: 25 posts debonded during removal of temporary restorations, and 16 teeth showed periapical lesions at the radiographic examination. No statistical significant differences were found among the four groups. The results of this retrospective study indicate that fiber posts in combination with bonding/luting materials can be routinely used.

Adult↗

Bonding to root canal: structural characteristics of the substrate.

PURPOSE: To evaluate in vitro the dentin morphology in root canals in terms of tubule orientation, density and increase in surface area after etching. MATERIALS AND METHODS: Thirty anterior teeth were divided in 3 groups at random: the samples of Group 1 were used to study tubular morphology in SEM. Groups 2 and 3 samples were etched with 32% phosphoric acid. The teeth in Group 2 were examined by SEM without further treatment. The samples in Group 3 were treated with a bonding system and fiber posts were luted into the canal. These teeth were then processed for evaluation of hybrid layer formation and resin tags in dentin tubules. The observations were made according to location in the root dentin, tubule density was estimated, and the increase in area available for bonding after etching was calculated. RESULTS: The observations revealed variability in tubule density and orientation within different areas of any one sample. Statistically significant differences in the density of tubules were found depending on location. The dentin surface area available for bonding increased by 202% after etching in the cervical third, 156% in the middle third, and 113% in the apical third of the root dentin. Group 3 samples showed that the thickness of the hybrid layer depended on the density of tubules. In the sectors with a low density of tubules, the hybrid layer was significantly thinner than in areas with a higher density of tubules. The increase in dentin surface area might be responsible for the enhanced bond strength after acid etching, but not all areas exhibited equal responses to etching.

Acid Etching, Dental↗

Confocal and scanning electron microscopic study of teeth restored with fiber posts, metal posts, and composite resins.

Forty-two single-rooted lower premolars, extracted for periodontal reasons, were endodontically treated and divided into 7 groups of 6 teeth each. In five of the groups, three different types of carbon fiber posts, quartz fiber posts, and titanium posts were used in combination with All Bond 2 dental adhesive. In two groups, two types of carbon fiber posts were also cemented with Panavia 21 dental adhesive. After 3 wk storage in saline, the teeth were longitudinally sectioned; one half was observed using confocal microscopy and the other by scanning electron microscopy. The specimens were evaluated for the presence of a resin dentin interdiffusion zone for the presence of voids at post-resin-dentin interfaces and for the determination of the fiber posts' structure. Upon examination with the confocal microscope, the interfaces of teeth restored with All Bond 2 showed a higher percentage (p < 0.05) of resin dentin interdiffusion zone than those treated with panavia. The fiber size and the post structure were similar in all the fiber posts observed. Some voids were present inside the fiber post structure.

Bicuspid↗

Dye leakage and SEM study of roots obturated with Thermafill and dentin bonding agent.

The apical seal of roots obturated with a dentin bonding agent and Thermafill with and without the use of sodium hypochlorite as an irrigating solution was compared by a dye leakage test. Roots obturated with Thermafill and a zinc oxide eugenol sealer were used as controls. Thirty-eight roots were prepared chemomechanically and divided into three experimental groups. The teeth of group 1 were filled with Thermafill and the dentin bonding agent using sodium hypochlorite as the irrigant. The teeth of group 2 were filled in the same way, but saline solution was used as the irrigant. Before the root canal was filled the smear layer was removed from the root canal walls of both groups by rinsing the root canal with a 17% EDTA solution. The teeth of group 3 were filled with Thermafill and a zinc oxide eugenol sealer. The teeth were immersed in 2% methylene blue solution. The root fillings of groups 1 and 2 leaked significantly more than those of group 3. The resin-dentin-guttapercha interface of group 1 was observed by scanning electron microscopy and showed a typical hybrid layer. An intimate contact between resin and dentin was present in group 2, but a resin-dentin interdiffusion zone was only occasionally observed. The use of dental adhesives and the hybrid layer formation did not improve the seal of Thermafill root canal fillings.

Coloring Agents↗

Standardized microscopic evaluation of the bonding mechanism of NRC/Prime & Bond NT.

PURPOSE: To investigate the in vitro formation of hybrid layer, resin tags and adhesive lateral branches, when using Prime & Bond NT (PBNT) "one-bottle" enamel-dentin bonding system on conditioned and unconditioned human dental substrates. MATERIALS AND METHODS: The enamel dentin adhesive was tested on 18 dentin and 18 enamel flat preparations made on facial and occlusal surfaces, respectively, of extracted posterior teeth. Each experimental tooth substrates were randomly divided in six groups of six samples each: Group 1: PBNT on conditioned dentin (CD) with 36% phosphoric acid (PA), Group 2: PBNT on etched enamel (EE) with 36% PA, Group 3: PBNT on CD with NRC non-rinse conditioning (NRC); Group 4: PBNT on EE with NRC, Group 5: PBNT on dentin without any conditioning (UD), Group 6: PBNT on enamel without any conditioning (UE). In the experimental groups, PBNT was applied following the manufacturer's instructions. Over the PBNT, a layer of Dyract AP was placed and light-cured for 20 seconds. All samples were split-fractured along their long axis. One half of each sample was deproteinized and decalcified at the interface in order to visualize the hybrid layer and the other was completely dissolved in order to observe the morphology of resin tags with a scanning electron microscope. RESULTS: Groups 1, 2: The tested samples showed a micromechanical bonding mechanism to CD and EE with phosphoric acid. PBNT formed hybrid layer, resin tags and adhesive lateral branches on dentin. In the dentin samples of the first group, characteristic reverse cone-shaped tags with their corresponding adhesive lateral branches were evident. At the enamel site, traditional pattern of etch enamel was always observed with resin tags formation. Groups 3, 4: When the bonding system was applied on dentin conditioned with NRC, a thin hybrid layer was formed, resin tags were also noted but their shape was narrower than that observed in Group 1. At the enamel site, the conditioned surface showed presence of an etched pattern and of resin tags. Groups 5, 6: When PBNT was applied without any previous conditioning, the hybrid layer and resin tags formation were not evident on dentin, and no bonding mechanism was developed with unetched enamel.

Acid Etching, Dental↗

In vitro leakage of resin-bonded all-porcelain crowns.

PURPOSE: The purpose of this study was to evaluate the sealing ability of single-unit all-porcelain crowns with margins placed in cementum-dentin and luted with two different cementing materials. MATERIALS AND METHODS: Twenty extracted molars, free of caries and restorations, were selected. The sample abutments were prepared in a standardized manner and then divided in two groups (n = 10) at random. Group 1: The cervical margin was located 1 mm below the CEJ and the crowns were luted with EBS Multi (Espe) bonding system in combination with Compolute 1.0 Aplicap (CLA 1.0, Espe) experimental resin cement. Group 2: The cervical margin was placed 1 mm below the CEJ, and Syntac bonding system (Vivadent) and Variolink II resin cement (Vivadent) were used. IPS-Empress (Ivoclar) crowns were made following manufacturer's instructions. After luting procedures and 2,500 thermal cycles (5 degrees and 55 degrees C), the samples were processed and evaluated for marginal leakage at the cervical site. After scoring dye penetration in group 1 and 2 samples, half of the sections were randomly selected in order to evaluate 1) resin cement thickness and 2) hybrid layer formation at the interface between adhesive material and dental substrate. The leakage data were statistically evaluated with the Kruskal-Wallis non-parametric analysis of variance (ANOVA) by ranks with Bonferroni alpha adjustment (p < 0.05). RESULTS: Sixty percent of the group 1 samples and 80% of group 2 showed leakage. In group 1, the dye penetration was often limited to the cervical margin site, while in group 2, it mainly extended to the axial walls. Statistically significant differences between samples of the two groups were not found. Samples of group 1 showed a cement thickness of 82 microns, while 75 microns was found in group 2. A hybrid layer and resin tag formation were evident in group 1. In group 2 samples, the hybrid layer formation was less evident and thinner than in group 1. CONCLUSIONS: The two adhesive systems performed similarly. The cervical margins of single-unit all-porcelain crowns must be considered as one of the weakest areas of this type of esthetic restoration.

Adult↗

Intermittent loading of teeth restored using quartz fiber, carbon-quartz fiber, and zirconium dioxide ceramic root canal posts.

PURPOSE: The purpose of the investigation was to compare the performances of teeth restored with quartz fiber, carbon-quartz fiber, and zirconium-dioxide posts covered with all-ceramic crowns when subjected to a cyclic loading test performed in a wet environment. MATERIALS AND METHODS: Forty single-rooted human lower premolars having similar dimensions were endodontically treated and mounted in acrylic resin blocks with a simulated periodontal ligament. The teeth were divided into three experimental groups and one control group. Post holes 8 mm long were prepared in the roots of the experimental groups in which quartz fiber, carbon-quartz fiber, and zirconium dioxide posts were cemented. In the control group, no posts were used. The crown buildup was made with composite resin. The teeth were covered with all-ceramic crowns and intermittently loaded at an angle of 45 degrees to the long axis of the tooth at a frequency of two loads per second. RESULTS: Only one failure (root fracture + post fracture) was observed in each of the fiber post groups, while in the zirconium dioxide post group, six failures were observed (one crown fracture and 5 root fractures + post fractures). The Kaplan-Meier analysis of the three experimental groups showed that the survival rate of zirconium dioxide posts was significantly lower than that of both types of fiber post. All the experimental groups showed a survival rate higher than that of the control group. CONCLUSION: Fiber posts reduced to a minimum the risk of root fractures of teeth restored with composite cores and Empress crowns under the present experimental conditions (intermittent loading in a wet environment).

Aluminum Silicates↗

Sealing ability of two "compomers" applied with and without phosphoric acid treatment for Class V restorations in vivo.

STATEMENT OF PROBLEM: "Compomers" are being used with increasing frequency in Europe and North America. PURPOSE: This study evaluated the marginal seal of two compomers in vivo. MATERIAL AND METHODS: Periodontally involved teeth, scheduled for extraction, were selected. Four groups of a combination of materials were used (Dyract with Dyract-PSA primer, group 1; Dyract with Prime and Bond 2.0, group 2; Compoglass with SCA primer, group 3; and Compoglass with Syntac Single Component, group 4). The restorations were made in a standardized shaped cavity, across the cementoenamel junction, and the teeth were extracted after 2 to 3 months of clinical service. The specimens were kept in a solution of 2% methylene blue for 24 hours. After being embedded in epoxy resin, sections were made with a low speed saw along the longitudinal axis of the teeth. The examination of dye penetration was made with a microscope at a magnification of x20 and scoring was done at both coronal and apical sites. Kruskal-Wallis statistical analysis was performed at 5% significance level. RESULTS: Groups 2 and 4 scored significantly less than groups 1 and 3 and for groups 2 and 4, 30% of the restorations exhibited leakage. CONCLUSION: These restoration systems did not completely prevent leakage either at the incisal or the cervical margins. Therefore the use of an enamel-dentin bonding system in combination with the proprietary compomer is recommended.

Acid Etching, Dental↗