Biomedical subjects
F Mizuho
Publications and source records attributed to F Mizuho.
Aspartate aminotransferase (AST) levels in human periodontium-derived cells.
The ability to objectively assess periodontal disease activity can significantly affect periodontal therapy. Aspartate aminotransferase (AST) is released extracellularly upon tissue destruction which suggests its potential as a key index in a quantitative assay for evaluating periodontal disease activity. The purpose of the present research was to assess the origin of AST in gingival crevicular fluid (GCF) in vitro. An experimental kit was used for the measurement of AST level in human gingival epithelial cells (HGEs), human gingival fibroblasts (HGFs), human periodontal ligament fibroblasts (HPLFs), polymorphonuclear leukocytes (PMNs), and plasma in peripheral blood. AST activity levels were observed in all of the periodontally derived cells, PMNs, and plasma. A significantly high level of AST activity was observed in HGEs (104.93 +/- 8.13 KU/1000 cells). The level of AST activity in HPLFs was 18.09 +/- 3.73 KU/1000 cells. AST activity in PMNs was significantly low, approximately 2% of that observed in HPLFs. These findings may suggest that AST level in GCF is correlated with the destruction of periodontal tissue.
[Trends in the use of antimicrobial agents in Kanagawa Dental College, Hospital].
The development and expansion of antimicrobial agents has made a remarkable improvement. Recently we began to use newqinolon agents, furthermore we began to use the third generation of oral cephem agents. We anticipate we will have a large change of trends in the use of antimicrobial agents. We investigated the trends in the use of antimicrobial agents between April 1st, 1983 to March 31st, 1988, to prepare for the trends in the future. 1) A total use of antimicrobial agents in Kanagawa Dental College, Hospital has increased 19% over the last five year. 2) Trends in the use of injection of antimicrobial agents has decreased over the same period of time, otherwise trends in the use of oral antimicrobial agents has increased tremendously 3) The use of newqinolon agents were less than 0.07% in one year.