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F Moody-Corbett

Publications and source records attributed to F Moody-Corbett.

21 records · Page 2Linked to original sources
PubMed1982

Increased adhesiveness at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve.

In culture, myotomal muscle cells from Xenopus laevis embryos develop discrete patches of high acetylcholine receptor (AChR) density. To examine the relative adhesiveness of these sites, muscle cells having AChR patches on their lower surface (apposed to the culture dish) were identified and were then treated with dibucaine or potassium-Ringer in order to cause the cells to round up. More than 90% of these cells remained attached at an AChR patch after rounding up, and this was the case even when the cells had a single patch on their lower surface. When the cells were torn away from the culture dish by mechanical agitation, small cellular fragments still remained firmly attached to the dish and many of these fragments contained an AChR patch. It is concluded that AChR patches on the lower surface of the cell are often located at sites of increased adhesiveness to the culture dish. The findings are discussed in terms of the formation and maintenance of AChR patches.

Animals↗
PubMed1981

Localization of cholinesterase at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve.

Myotomal muscle cells, derived from Xenopus embryos and grown in culture without nerve, develop discrete sites of cholinesterase (ChE) activity on their surface. The spatial relationship of these ChE patches to surface patches of acetylcholine receptors (AChRs) has been examined in the present study by a combination of ChE histochemistry and fluorescent staining of the receptors. ChE patches and AChR patches developed as early as the 1st day in culture and exhibited a high incidence of spatial overlap. The frequency of overlap varied with the age of the culture and ranged from 50 to 98% for patches on the lower cell surface (facing the floor of the culture dish) and from 28 to 79% for patches on the upper cell surface. The high incidence of overlap cannot be explained on the basis of a random distribution of patches since both types of patch occupied less than 3% of the cell surface. ChE and AChR patches also developed when cultures were grown in a serum-free medium as well as when cultures were prepared from young embryos in which muscle innervation had not yet begun. At some patches, the surface membrane was invaginated and at these invaginations, there was also a high incidence of overlap between the ChE and AChR stains. It is concluded that the mechanisms involved in the localization of AChRs and ChE on the surface of Xenopus myotomal muscle cells tend to be closely linked and operate even in the absence of innervation, previous contact by nerve, or electrical and contractile activity. Considered together with previous ultrastructural observations, the present findings suggest that these cells develop elaborate postsynaptic-like specializations even in the absence of neural factors.

Animals↗