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Biomedical subjects

F Negretti

Publications and source records attributed to F Negretti.

At least 19 recordsLinked to original sources
PubMed1992

Study on the influence of adsorbent substances against the antigen activity of inactivated orovaccines.

Specific IgAGM, IgA and IgG levels were evaluated in two groups of rabbits orally treated with inactivated vaccine consisting of Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Streptococcus faecalis and with the same vaccine supplemented with charcoal and kaolin, respectively. The determinations demonstrated that charcoal and kaolin do not have a significant, negative effect on immunoresponses at the intestinal and hematic level after both medium (two-week) and prolonged (four-week) treatment.

Administration, Oral↗
PubMed1992

The microbial controls of antiseptics and disinfectants by membrane filtration: experimental evaluation of the interferences.

Microbiological investigations made by membrane filtration method on antiseptics and disinfectants demonstrated that the filtering membranes present very frequently a remarkable antimicrobial activity, even after washing with 300 ml of peptone water according to the guidelines of the Pharmacopoeia. The observed phenomena (appearance of inhibition zones, disappearance or decrease of the number of colonies forming units, release of antimicrobial properties to the cultural broths) can seriously diminish the validity of microbiological controls of antiseptics and disinfectants.

Anti-Infective Agents, Local↗
PubMed1990

Remarkable increases of salivary IgE levels in allergic syndromes.

Serum and salivary IgE levels were tested by indirect ELISA in patients with allergic contact dermatitis and allergic asthma or rhinitis. The remarkable increases of salivary IgE (from 928.4% mean augmentation in allergic asthma or rhinitis to 2686.2% in allergic contact dermatitis) suggest the idea of using saliva in IgE titration.

Humans↗
PubMed1990

[Experimental study of the course of the immune response at the urinary level following repeated oral vaccination].

The Progression of the specific immunoreactions in the urines of rabbits vaccinated by oral route from 1 to 10 times with 20-40 billion of some antigens (E. coli, Proteus vulgaris, Staph aureus, Str. faecalis), administered daily or by-weekly, has been evaluated by agglutination and ELISA. The immunoenzymatic ELISA technique has revealed increases of specific immunoglobulins also one day after the first oral administration of the antigens and a final progression of IgA titres (after ten treatments) from 81% to 798%, according to the various antigens and the different posology. The highest values have been obtained using the posology of 40 billion and the bi-weekly sequence.

Administration, Oral↗
PubMed1977

[Studies of the microbial content of raw materials used in pharmaceutical preparations].

The microbiological controls (determination of the total and mycetic bacterial count; isolation of enterococci, Clostridium, Pseudomonas aeruginosa, Staph. aureus, E. coli, Salmonella and other Enterobacteriaceae) executed on 1517 samples of 100 varous raw materials, have ascertained the presence of a microbial contamination varying to matters, with presence also of pathogenic or hygienically undesirable microrganisms. The more contaminated substances have resulted to be those organic either of vegetable or animal origin. Sensible differences have been noted between the various lots and according to the various suppliers. The conservation trials under various conditions of temperature (15-22-37 degrees C) and humidity (30 degrees - 60 degrees - 90 degrees of relative humidity) with opened or closed recipients, have demonstrated the possibility of remarkable increases of the number of the microrganisms in the raw materials with increase of temperature and humitidy, especially in the non hermatically closed recipients.

Bacteria↗
PubMedDate not supplied

Microbiological control of pharmaceutical products containing preservatives: drawbacks of the method of membrane filtration.

Microbiological assays carried out on membranes employed for the filtration of solutions of preservatives with concentration used in pharmaceuticals, demonstrated that such membranes, even after washing with 300 ml (100 ml X 3) of peptone water according to the guidelines of the Pharmacopoeia, maintain very frequently a remarkable antimicrobial activity revealed by inhibition zones and by disappearance or decrease of the number of colonies forming units. Such drawbacks can interfere negatively with the microbiological controls of pharmaceuticals containing preservatives.

Drug Contamination↗
PubMedDate not supplied

[The sequence of specific immune reactions at the biliary level after oral vaccination].

The appearance and the progression of the specific immunoreactions at biliary levels (agglutinant titre, immunoenzimatic titration of IgAGM, IgA, IgG) in rabbits treated per os from 1 to 10 times, daily or bi-weekly, with 20 and 40 milliard of inactivated bacterial antigens (E. coli, Proteus vulgaris, Staph. aureus, Str. faecalis) have been studied. The appearance of the specific biliary antibodies has been very precocious (only one day after the first administration) and the values increased progressively with the number of administrations. The posology of 40 milliard and the bi-weekly sequence of the treatments presented the highest immunoreactions. In the bile the IgA and IgG increased considerably, but the IgA have been preponderant; in the serum only the IgG presented a remarkable increase, not the IgA, that are present in very small quantity.

Administration, Oral↗