Biomedical subjects

F P T Baaijens

Publications and source records attributed to F P T Baaijens.

At least 19 recordsLinked to original sources

PubMed2006

Mechanical properties and failure of Streptococcus mutans biofilms, studied using a microindentation device.

Knowledge of mechanical properties and failure mechanisms of biofilms is needed to determine how biofilms react on mechanical stress. Methods currently available cannot be used to determine mechanical properties of biofilms on a small scale with high accuracy. A novel microindentation apparatus in combination with a confocal microscope was used to determine the viscoelastic properties of Streptococcus mutans biofilms. The apparatus comprises a small glass indenter and a highly sensitive force transducer. It was shown that the present biofilm, grown under still conditions, behaves as a viscoelastic solid with a storage modulus of 1-8 kPa and a loss modulus of 5-10 kPa at a strain of 10%. Biofilm failure was investigated visually through a confocal microscope by dragging the indenter through the biofilm. It was shown that the tensile strength of the biofilm is predominantly determined by the tensile strength of the extracellular polysaccharide matrix. The combination of microindentation and confocal microscopy is a promising technique to determine and characterize the mechanical properties of soft materials in various fields of microbiology.

Bacteriological Techniques↗

PubMed2006

Electrospinning versus knitting: two scaffolds for tissue engineering of the aortic valve.

Two types of scaffolds were developed for tissue engineering of the aortic valve; an electrospun valvular scaffold and a knitted valvular scaffold. These scaffolds were compared in a physiologic flow system and in a tissue-engineering process. In fibrin gel enclosed human myofibroblasts were seeded onto both types of scaffolds and cultured for 23 days under continuous medium perfusion. Tissue formation was evaluated by confocal laser scanning microscopy, histology and DNA quantification. Collagen formation was quantified by a hydroxyproline assay. When subjected to physiologic flow, the spun scaffold tore within 6 h, whereas the knitted scaffold remained intact. Cells proliferated well on both types of scaffolds, although the cellular penetration into the spun scaffold was poor. Collagen production, normalized to DNA content, was not significantly different for the two types of scaffolds, but seeding efficiency was higher for the spun scaffold, because it acted as a cell impermeable filter. The knitted tissue constructs showed complete cellular in-growth into the pores. An optimal scaffold seems to be a combination of the strength of the knitted structure and the cell-filtering ability of the spun structure.

Aortic Valve↗

PubMed2005

The relative contributions of different skin layers to the mechanical behavior of human skin in vivo using suction experiments.

Although the mechanical behavior of the top layer of the skin, the epidermis, is an important consideration in several clinical and cosmetic applications, there are few reported studies on this layer. The in vivo mechanical behavior of the upper skin layer (here defined as epidermis and papillar dermis) was characterized using a combined experimental and modeling approach. The work was based on the hypothesis that experiments with different length scales represent the mechanical behavior of different skin layers. Suction measurements with aperture diameters of 1, 2 and 6 mm were combined with ultrasound and optical coherence tomography to study the deformation of the skin layers. The experiments were simulated for small displacements with a two-layered finite element model representing the upper layer and the reticular dermis. An identification method compared the experimental and numerical results to identify the material parameters of the model. For one subject the whole parameter estimation procedure was completed, leading to a stiffness of C(10,ul) = 0.11 kPa for the top-layer and C(10,rd) = 0.16 MPa for the reticular dermis. This unexpected, extreme stiffness ratio of the material parameters let to convergence problems of the finite element software for most of the individuals.

Adult↗

PubMed2005

Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique.

Aiming to develop a scaffold architecture mimicking morphological and mechanically that of a blood vessel, a sequential multi-layering electrospinning (ME) was performed on a rotating mandrel-type collector. A bi-layered tubular scaffold composed of a stiff and oriented PLA outside fibrous layer and a pliable and randomly oriented PCL fibrous inner layer (PLA/PCL) was fabricated. Control over the level of fibre orientation of the different layers was achieved through the rotation speed of the collector. The structural and mechanical properties of the scaffolds were examined using scanning electron microscopy (SEM) and tensile testing. To assess their capability to support cell attachment, proliferation and migration, 3T3 mouse fibroblasts and later human venous myofibroblasts (HVS) were cultured, expanded and seeded on the scaffolds. In both cases, the cell-polymer constructs were cultured under static conditions for up to 4 weeks. Environmental-scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), histological examination and biochemical assays for cell proliferation (DNA) and extracellular matrix production (collagen and glycosaminoglycans) were performed. The findings suggest the feasibility of ME to design scaffolds with a hierarchical organization through a layer-by-layer process and control over fibre orientation. The resulting scaffolds achieved the desirable levels of pliability (elastic up to 10% strain) and proved to be capable to promote cell growth and proliferation. The electrospun PLA/PCL bi-layered tube presents appropriate characteristics to be considered a candidate scaffold for blood vessel tissue engineering.

3T3 Cells↗

PubMed2005

Mechanical and failure properties of single attached cells under compression.

Eukaryotic cells are continuously subjected to mechanical forces under normal physiological conditions. These forces and associated cellular deformations induce a variety of biological processes. The degree of deformation depends on the mechanical properties of the cell. As most cells are anchorage dependent for normal functioning, it is important to study the mechanical properties of cells in their attached configuration. The goal of the present study was to obtain the mechanical and failure properties of attached cells. Individual, attached C2C12 mouse myoblasts were subjected to unconfined compression experiments using a recently developed loading device. The device allows global compression of the cell until cell rupture and simultaneously measures the associated forces. Cell bursting was characterized by a typical reduction in the force, referred to as the bursting force. Mean bursting forces were calculated as 8.7+/-2.5 microN at an axial strain of 72+/-4%. Visualization of the cell using confocal microscopy revealed that cell bursting was preceded by the formation of bulges at the cell membrane, which eventually led to rupturing of the cell membrane. Finite element calculations were performed to simulate the obtained force-deformation curves. A finite element mesh was built for each cell to account for its specific geometrical features. Using an axisymmetric approximation of the cell geometry, and a Neo-Hookean constitutive model, excellent agreement between predicted and measured force-deformation curves was obtained, yielding an average Young's modulus of 1.14+/-0.32 kPa.

Animals↗

PubMed2004

A computational model for collagen fibre remodelling in the arterial wall.

As the interaction between tissue adaptation and the mechanical condition within tissues is complex, mathematical models are desired to study this interrelation. In this study, a mathematical model is presented to investigate the interplay between collagen architecture and mechanical loading conditions in the arterial wall. It is assumed that the collagen fibres align along preferred directions, situated in between the principal stretch directions. The predicted fibre directions represent symmetrically arranged helices and agree qualitatively with morphometric data from literature. At the luminal side of the arterial wall, the fibres are oriented more circumferentially than at the outer side. The discrete transition of the fibre orientation at the media-adventitia interface can be explained by accounting for the different reference configurations of both layers. The predicted pressure-radius relations resemble experimentally measured sigma-shaped curves. As there is a strong coupling between the collagen architecture and the mechanical loading condition within the tissue, we expect that the presented model for collagen remodelling is useful to gain further insight into the processes involved in vascular adaptation, such as growth and smooth muscle tone adaptation.

Adaptation, Physiological↗

PubMed2004

The local matrix distribution and the functional development of tissue engineered cartilage, a finite element study.

Assessment of the functionality of tissue engineered cartilage constructs is hampered by the lack of correlation between global measurements of extra cellular matrix constituents and the global mechanical properties. Based on patterns of matrix deposition around individual cells, it has been hypothesized previously, that mechanical functionality arises when contact occurs between zones of matrix associated with individual cells. The objective of this study is to determine whether the local distribution of newly synthesized extracellular matrix components contributes to the evolution of the mechanical properties of tissue engineered cartilage constructs. A computational homogenization approach was adopted, based on the concept of a periodic representative volume element. Local transport and immobilization of newly synthesized matrix components were described. Mechanical properties were taken dependent on the local matrix concentration and subsequently the global aggregate modulus and hydraulic permeability were derived. The transport parameters were varied to assess the effect of the evolving matrix distribution during culture. The results indicate that the overall stiffness and permeability are to a large extent insensitive to differences in local matrix distribution. This emphasizes the need for caution in the visual interpretation of tissue functionality from histology and underlines the importance of complementary measurements of the matrix's intrinsic molecular organization.

Biomechanical Phenomena↗

PubMed2004

Collagen fibers reduce stresses and stabilize motion of aortic valve leaflets during systole.

The effect of collagen fibers on the mechanics and hemodynamics of a trileaflet aortic valve contained in a rigid aortic root is investigated in a numerical analysis of the systolic phase. Collagen fibers are known to reduce stresses in the leaflets during diastole, but their role during systole has not been investigated in detail yet. It is demonstrated that also during systole these fibers substantially reduce stresses in the leaflets and provide smoother opening and closing. Compared to isotropic leaflets, collagen reinforcement reduces the fluttering motion of the leaflets. Due to the exponential stress-strain behavior of collagen, the fibers have little influence on the initial phase of the valve opening, which occurs at low strains, and therefore have little impact on the transvalvular pressure drop.

Animals↗

PubMed2004

A finite element model of the human buttocks for prediction of seat pressure distributions.

Seating comfort is becoming increasingly important for the automotive industry. Car manufacturers use seating comfort to distinguish their products from those of competitors. However, the development and design of a new, more comfortable seat is time consuming and costly. The introduction of computer models of human and seat will accelerate this process. The contact interaction between human and seat is an important factor in the comfort sensation of subjects. This paper presents a finite element (FE) model of the human buttocks, able to predict the pressure distribution between human and seating surface by its detailed and realistic geometric description. A validation study based on volunteer experiments shows reasonable correlation in pressure distributions between the buttocks model and the volunteers. Both for simulations on a rigid and a soft cushion, the model predicts realistic seat pressure distributions. A parameter study shows that a pressure distribution at the interface between human and seat strongly depends on variations in human flesh and seat cushion properties.

Adult↗

PubMed2004

Influence of hydration and experimental length scale on the mechanical response of human skin in vivo, using optical coherence tomography.

BACKGROUNDS/AIMS: Human skin is a complex tissue consisting of different layers. To gain better insight into the mechanical behaviour of different skin layers, the mechanical response was studied with experiments of various length scales. Also, the influence of (superficial) hydration on the mechanical response is studied. The work is based on the hypothesis that experiments with different length scales represent the mechanical behaviour of different skin layers. For suction, this means that a large aperture diameter reflects the behaviour of mainly dermis, whereas a very small diameter reflects the behaviour of only the top layer of the skin. METHODS: Suction measurements at varying pressures and aperture sizes were performed on the volar forearm of 13 subjects aged 29-47 years. The deformation of the skin was visualized using ultrasound (US) (dermis) and optical coherence tomography (OCT) (epidermis and dermis). US measurements were performed on hydrated skin, OCT measurements on dry and hydrated skin. The experiment was simulated by a finite element model (FEM) exhibiting extended Mooney material behaviour. An identification method was used to compare the experimental and numerical results to identify the parameters of the material. RESULTS: The material parameters C(10) and C(11) were calculated for four subjects: C(10)=29.6+/-21.1 kPa and C(11)=493+/-613 kPa for 6 mm aperture diameter, C(10)=11.5+/-8.7 kPa and C(11)=18.3+/-12.6 kPa for 2 mm aperture diameter and C(10)=10.8+/-9.5 kPa and C(11)=9.3+/-7.7 kPa for 1 mm aperture diameter. Skin hydration caused ambiguous effects on the mechanical response. CONCLUSIONS: US and OCT, combined with suction, using varying apertures sizes, proved to be a valuable tool to study the mechanical behaviour of different skin layers. With increasing experimental length scale, increasing values for the parameters of the material model were found. This indicates the need of a multi-layered material layer FEM, which can be used to identify mechanical behaviour of epidermis and dermis.

Adult↗

PubMed2003

Quantifying pressure sore-related muscle damage using high-resolution MRI.

To obtain insight into the etiology of deep pressure sores, understanding of the relationship between prolonged transverse loading and local muscle damage is required. To date, the amount and location of muscle damage have been determined by histological examination. In the present study, we determined whether T2-weighted high-resolution magnetic resonance imaging (MRI) can also be applied to evaluate muscle tissue after prolonged transverse loading. The tibialis anterior muscle and overlying skin in the right hindlimbs of five rats were compressed between an indenter and the tibia. The in vivo magnetic resonance images of the loaded and contralateral hindlimbs were obtained 24 h after load application. The tibialis anterior muscles were then processed for histological examination. In the magnetic resonance images of all five loaded hindlimbs, signal intensity appeared higher in the loaded regions of the muscle compared with the unloaded regions. The location of the higher signal intensity coincided with the location of damage assessed from histology. Also the amount of damage determined with MRI was in good agreement with the amount of damage assessed from histological examination. Because MRI is nondestructive, it is a promising alternative for histology in research on pressure sore etiology, especially in follow-up studies to evaluate the development of muscle damage in time and in clinical studies.

Animals↗

PubMed2003

Monitoring the biomechanical response of individual cells under compression: a new compression device.

Skeletal muscle cells are sensitive to sustained compression, which can lead to the development of pressure sores. Although it is known that this type of tissue breakdown depends on the magnitude and duration of the applied load, the exact relationship between cell deformation and damage remains unclear. To gain more insight into this process, a method has been developed, that incorporates the use of a new loading device and confocal microscopy. The loading device is able to compress individual cells, either statically or dynamically, while measuring the resulting forces. Experiments can be performed under ideal environmental conditions, comparable with those of a CO2 incubator. First compression experiments on C2C12 mouse myoblasts showed the shape changes that cells undergo during static compression by the loading device. Calculations using the three-dimensional confocal images showed no change in volume and an increase in the surface area of the cell as a result of compression. The device presented here provides a useful way to monitor the biomechanical response of skeletal muscle cells during long-term compression experiments. Therefore it will contribute to the knowledge about strain-induced cell damage, as seen in pressure sores and other mechanically induced clinical conditions.

Animals↗

PubMed2003

A three-dimensional computational analysis of fluid-structure interaction in the aortic valve.

Numerical analysis of the aortic valve has mainly been focused on the closing behaviour during the diastolic phase rather than the kinematic opening and closing behaviour during the systolic phase of the cardiac cycle. Moreover, the fluid-structure interaction in the aortic valve system is most frequently ignored in numerical modelling. The effect of this interaction on the valve's behaviour during systolic functioning is investigated. The large differences in material properties of fluid and structure and the finite motion of the leaflets complicate blood-valve interaction modelling. This has impeded numerical analyses of valves operating under physiological conditions. A numerical method, known as the Lagrange multiplier based fictitious domain method, is used to describe the large leaflet motion within the computational fluid domain. This method is applied to a three-dimensional finite element model of a stented aortic valve. The model provides both the mechanical behaviour of the valve and the blood flow through it. Results show that during systole the leaflets of the stented valve appear to be moving with the fluid in an essentially kinematical process governed by the fluid motion.

Aortic Valve↗

PubMed2003

A computational fluid-structure interaction analysis of a fiber-reinforced stentless aortic valve.

The importance of the aortic root compliance in the aortic valve performance has most frequently been ignored in computational valve modeling, although it has a significant contribution to the functionality of the valve. Aortic root aneurysm or (calcific) stiffening severely affects the aortic valve behavior and, consequently, the cardiovascular regulation. The compromised mechanical and hemodynamical performance of the valve are difficult to study both 'in vivo' and 'in vitro'. Computational analysis of the valve enables a study on system responses that are difficult to obtain otherwise. In this paper a numerical model of a fiber-reinforced stentless aortic valve is presented. In the computational evaluation of its clinical functioning the interaction of the valve with the blood is essential. Hence, the blood-tissue interaction is incorporated in the model using a combined fictitious domain/arbitrary Lagrange-Euler formulation, which is integrated within the Galerkin finite element method. The model can serve as a diagnostic tool for clinical purposes and as a design tool for improving existing valve prostheses or developing new concepts. Structural mechanical and fluid dynamical aspects are analyzed during the systolic course of the cardiac cycle. Results show that aortic root compliance largely influences the valve opening and closing configurations. Stresses in the delicate parts of the leaflets are substantially reduced if fiber-reinforcement is applied and the aortic root is able to expand.

Aortic Valve↗

PubMed2003

Spatial and temporal heterogeneity of superficial muscle strain during in situ fixed-end contractions.

Numerical models of contracting muscle offer a powerful tool to study local mechanical load. For validation of these models, the spatial and temporal distribution of strain was quantified in fixed-end contracting rat tibialis anterior muscle in situ at optimal muscle length (L(o)) and at 120 degrees plantar flexion as well as at 125 and 33Hz stimulation frequency. We studied the hypothesis that after termination of stimulation in situ muscle segments near the motor endplates elongate while segments away from the endplates shorten. We show that both spatial and temporal inhomogeneities in muscle deformation occurred during contraction. Muscle plateau shortening strain equalled 4.1%. Maximal plateau shortening of a muscle segment was much larger (9.6%) and occurred distally (at 0.26 of the scaled length of the muscle). Manipulating torque levels by decreasing the stimulation frequency at the same muscle length induced a decrease in torque ( approximately 20%) with a smaller effect on the level and no effect on the pattern of muscle deformation. During relaxation, distal segments actively shortened at the expense of proximal muscle segments, which elongated. The segments undergoing lengthening were nearer to motor endplates than segments undergoing shortening. In conclusion, the present study provides experimental data on magnitude of contraction-induced deformation needed for validation of numerical models. Local muscle deformation is heterogeneous both temporally and spatially and may be related to proximity to the motor endplates.

Animals↗

PubMed2003

Remodelling of continuously distributed collagen fibres in soft connective tissues.

Extracellular matrix remodelling plays an essential role in tissue engineering of load-bearing structures. The goal of this study is to model changes in collagen fibre content and orientation in soft connective tissues due to mechanical stimuli. A theory is presented describing the mechanical condition within the tissue and accounting for the effects of collagen fibre alignment and changes in fibre content. A fibre orientation tensor is defined to represent the continuous distribution of collagen fibre directions. A constitutive model is introduced to relate the fibre configuration to the macroscopic stress within the material. The constitutive model is extended with a structural parameter, the fibre volume fraction, to account for the amount of fibres present within the material. It is hypothesised that collagen fibre reorientation is induced by macroscopic deformations and the amount of collagen fibres is assumed to increase with the mean fibre stretch. The capabilities of the model are demonstrated by considering remodelling within a biaxially stretched cube. The model is then applied to analyse remodelling within a closed stented aortic heart valve. The computed preferred fibre orientation runs from commissure to commissure and resembles the fibre directions in the native aortic valve.

Adaptation, Physiological↗

PubMed2003

A numerical-experimental method to characterize the non-linear mechanical behaviour of human skin.

BACKGROUND/AIMS: Human skin is a complex tissue consisting of several distinct layers. Each layer consists of various components with a specific structure. To gain a better insight into the overall mechanical behaviour of the skin, we wish to study the mechanical properties of the different layers. A numerical-experimental method was developed to characterize the non-linear mechanical behaviour of human dermis. METHODS: Suction measurements at varying pressures were performed on the volar forearm skin of 10 subjects aged 19-24 years old. Deformation of dermis and fat during suction was measured using ultrasound. The experiment was simulated by a finite element model exhibiting extended Mooney material behaviour to account for the non-linear stress-strain relationship. An identification method is used to compare the experimental and numerical results to identify the parameters of the material model. RESULTS: C10, dermis was found to be 9.4 +/- 3.6 kPa and C11, dermis to be 82 +/- 60 kPa. A first rough estimate of C10, fat was 0.02 kPa. CONCLUSIONS: The resulting finite element model demonstrated its ability to describe the response of the skin to suction at various pressures. In the future, this method can be used to characterize the mechanical behaviour of different skin layers using various aperture sizes and to characterize the skin behaviour under various loading conditions.

Adipose Tissue↗

PubMed2003

The relevance of large strains in functional tissue engineering of heart valves.

BACKGROUND: Exposing the developing tissue to flow and pressure in a bioreactor has been shown to enhance tissue formation in tissue-engineered heart valves. Animal studies showed excellent functionality in these valves in the pulmonary position. However, they lack the mechanical strength for implantation in the high-pressure aortic position. Improving the in vitro conditioning protocol is an important step towards the use of these valves as aortic heart valve replacements. In this study, the relevance of large strains to improve the mechanical conditioning protocol was investigated. METHODS: Using a newly developed device, engineered heart valve tissue was exposed to increasing cyclic strain in vitro. Tissue formation and mechanical properties were analyzed and compared to unstrained controls. RESULTS: Straining resulted in more pronounced and organized tissue formation with superior mechanical properties over unstrained controls. Overall tissue properties improved with increasing strain levels. CONCLUSIONS: The results demonstrate the significance of large strains in promoting tissue formation. This study may provide a methodological basis for tissue engineering of heart valves appropriate for systemic pressure applications.

Absorbable Implants↗