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Biomedical subjects

F Perán

Publications and source records attributed to F Perán.

11 recordsLinked to original sources
PubMed1996

Lymphocyte subpopulations and catecholamines; daytime variations and relationships.

The diurnal variations and their possible relations with endogenous catecholamines have been studied in 16 apparently healthy subjects (8 women and 8 men, between 22-35 years of age). A butterfly cannula was inserted into the flexure vein of the elbow at 8 h and at 20 h, a blood sample being taken after three 15-minute intervals. Leukocytes, lymphocytes, monocytes, and granulocytes were quantified in a cell counter while lymphocyte subpopulations were determined with flow cytometry in cells labelled with specific monoclonal antibodies. Plasma catecholamine concentrations were measured by high-pressure liquid chromatography. Significant differences for leukocyte circulating levels were found, as well as for all subpopulations measured at different times of day, save NK cells and monocytes. A statistically significant correlation was also found both between leukocytes, all subpopulations and epinephrine save B lymphocytes and NK cells, and between total lymphocytes, T lymphocytes, CD4, CD4/45RA+ subtypes and norepinephrine. In conclusion, diurnal rhythms were proved to exist in leukocyte and lymphocyte subpopulations; the significant correlation between these cells and catecholamine plasma levels suggests the presence of a possible mechanism that connects the cellular immunity with determined hormones.

Adult↗
PubMed1995

CA 125, CA 15.3, CA 27.29, CEA, beta-hCG and alpha-fetoprotein levels in cyst fluid of breast macrocysts.

OBJECTIVE: To determine the tumoral markers CA 125, CA 15.3, CA 27.29, CEA, beta-hCG and alpha-fetoprotein in serum and breast cystic fluid in women with fibrocystic breast disease. METHODS: The study comprised 50 women diagnosed with fibrocystic breast disease but without breast macrocysts, and 60 women with macrocysts. RESULTS: Significantly higher levels of CA 125, CA 27.29, beta-hCG and CEA were observed in the cyst fluid than in the serum of the same patients. According to their K+/Na+ quotient we found that when K+/Na+ was < 3, cyst fluid CA 125, CA 27.29, beta-hCG and CEA levels were significantly higher than serum levels. However, when K+/Na+ was > 3, only CA 27.29 and beta-hCG levels were significantly higher. Comparing cyst fluid marker levels with respect to the K+/Na+ ratio, the only difference observed was in CA 125 which was significantly greater when K+/Na+ was < 3. A negative correlation was found between CA 125 and the K+/Na+ quotient. CONCLUSION: These results suggest that CA 125 could be used as a marker to identify cyst type.

17-alpha-Hydroxyprogesterone↗
PubMed1995

Changes in tissue calcium and phosphorus content and plasma concentrations of parathyroid hormone and calcitonin after long-term magnesium deficiency in rats.

OBJECTIVE: The present study was undertaken to investigate the effect of dietary magnesium (Mg) deficiency on plasma levels of parathyroid hormone (PTH) and calcitonin (CT), and the changes in tissue calcium (Ca) and phosphorus (P) content. METHODS: Wistar rats were fed an Mg-deficient diet. Plasma levels of PTH and CT, and Ca and P content in plasma, whole blood, skeletal muscle, heart, kidney, femoral bone and brain, were determined after 7, 35, 42, 49, 56, 63 and 70 days. RESULTS: Mg deficiency significantly increased plasma levels of ionic Ca after 1 week of deficiency, and significantly increased total Ca in plasma and whole blood after 5 weeks. Whole blood and plasma levels of P decreased significantly after 8 and 5 weeks, respectively, in comparison with values obtained in pair-fed control rats. Plasma levels of CT were not modified by hypercalcemia and hypomagnesemia. Plasma PTH decreased significantly during the first 3 weeks, then recovered gradually, reaching control values in week 7 and remaining stable thereafter, despite elevated levels of ionic Ca. The Mg-deficient diet led to calcification in skeletal muscle, heart, kidney and femur, detectable as early as week 5. However, P content increased only in kidney after 1 week, decreased in heart and femur after 5 weeks, and did not change in muscle. The Mg-deficient diet did not significantly modify Ca or P content in brain. CONCLUSION: Mg deficiency led to tissue calcification and decreased P levels in blood, heart, and femur. These changes did not affect CT secretion. PTH appeared to be more closely related with hypomagnesemia than with hypercalcemia.

Animals↗
PubMed1994

Upmodulation by estrogen of HLA class I expression in breast tumor cell lines.

The expression of major histocompatibility complex (MHC) class I antigens was studied in five breast carcinoma cell lines before and after treatment with 17-beta estradiol. Increased HLA class I antigen expression correlated with the presence of estrogen receptors. The modulation of expression appeared to be mediated by transcriptional mechanisms, as revealed by class I mRNA levels. To elucidate the basis of MHC class I upregulation, we examined transcriptional factor binding activity to the class I regulatory element (CRE). Our results showed that 17-beta estradiol induced increases in factor binding activity to the CRE II probe, and decreases to the CRE I probe. In addition, our results suggested that factors that bind the CRE I region may modulate the binding of CRE II. Binding to CRE II was significantly increased in extracts pretreated with a competitor that contained the CRE I sequence, and that bound NF-kB/kBF1. In addition, induction of NF-kB binding activity by the tumor necrosis factor was accompanied by a decrease in nuclear factors that bind to the CRE II region.

Base Sequence↗
PubMed1992

[Diurnal variations in circulating leukocytes and subsets: relation to plasma cortisol and ACTH].

Surface cellular antigens of leukocytes, lymphocytes and corresponding subpopulations have been analysed by using monoclonal antibodies marked with fluorescein (PITC), parallel to those marked with phycoerythrin (PE). Cortisol and ACTH plasmatics have also been determined through RIA, on two samples at 8am. and 8pm. During this twelve hour evolution, a highly significant dependence of the leukocytes, T, T4 and T8 lymphocytes on the circulating ACTH has also been found. In general during the experiment time leukocytes, lymphocytes and subpopulations, have experimented an increase which is significantly related to the pituitary hormone secretion. The existence of this significant correlation establishes the presence of a possible mechanism that connects the cellular immunity to determined hypothalamus hormones.

Adrenocorticotropic Hormone↗
PubMed1990

Influence of hydrocortisone acetate administered to the lactating rat on protein and lactose content in milk and serum protein, glucose and insulin levels in dams and pups.

The influence of i.m. administration to the mother of hydrocortisone acetate (doses of 0.4, 0.8 or 2.0 mg/100 g body weight/day) during the first 15 days of lactation on milk protein and lactose composition and serum levels of protein, glucose and insulin in dams and pups is studied. Total serum proteins and albumin/globulin ratio in dams were unchanged by treatment. The daily injection of 0.4 or 0.8 mg/100 g body weight failed to alter serum levels of glucose or insulin in dams, whereas a dose of 2.0 mg/100 g body weight led to a rise in glucemia (from 118 +/- 3.2 to 133 +/- 5.3) which was accompanied by a sharp change in insulinemia (from 40.7 +/- 4.1 to 83.6 +/- 6.9). All three doses raised protein levels in milk. The smallest increase was recorded with 2.0 mg/100 g body weight; this dose also reduced milk lactose content. Total serum proteins in pups rose slightly but nonsignificantly, and no significant effects were noted on albumin/globulin ratio or serum glucose and insulin levels.

Animals↗
PubMed1985

Plasmatic beta-endorphin levels and thalamic surgery for pain.

Plasma beta-endorphin levels were found to be significantly lower in patients suffering from chronic pain of malignant etiology than in a control group. After a bilateral stereotactic cryothalamotomy in Centrum Medianum and Parafascicularis nuclei, a good clinical result and a significant increase in plasma beta-endorphin levels were obtained.

Adult↗
PubMed1984

Correlation between plasmatic and CSF beta-endorphin levels.

Fifty-four concomitant pairs of plasma and ventricular CSF samples were obtained in eighteen patients taking advantage of some neuroradiological or therapeutical procedures. A significant linear correlation between plasma and CSF beta-Endorphin levels has been found. The results obtained and those of other papers on the subject are discussed.

Adolescent↗
PubMedDate not supplied

[Evaluation of the hemodynamic and endocrino-metabolic response to tracheal intubation in patients anesthetized with thiopental or propofol].

OBJECTIVES: To compare the effects of thiopental and propofol on hemodynamic and metabolic endocrine response to laryngoscopy and intubation. MATERIAL AND METHODS: We selected two homogeneous groups of 14 healthy patients premedicated with midazolam i.m. (0.07 mg/kg). Induction was with diazepam (0.1 mg/kg), fentanyl (2 micrograms/kg), atropine 0.5 mg and thiopental or propofol (4-6.5 mg/kg and 1.5-2.5 mg/kg, respectively). Parameters recorded were direct arterial pressure, baseline heart rate, and heart rate after induction and at 2 and 5 minutes after intubation. We measured adrenaline (A), noradrenaline (NA), dopamine (Da), glucagon, beta-endorphines, ACTH, cortisol, glucose and amino acids in the baseline and post-intubation blood samples. RESULTS: We observed a significant increase (p < 0.05) in systolic and diastolic arterial pressure after intubation (10% and 22% respectively) in the thiopental group as compared with the propofol group. With both induction agents, heart rate was higher than baseline values at the three times (p < 0.001). In the thiopental group heart rate was higher after intubation than after induction (p < 0.05). Cortisol fell after intubation in the propofol group (p < 0.05); no other hormonal differences were observed. Hyperglycemia (p < 0.0001) was similar for both groups, while in the propofol group there were significant decreases in several amino acids. CONCLUSIONS: Propofol has a greater mitigating effect on the hyperdynamic response to intubation in healthy patients. For the two induction agents we measured no significant differences in stress hormone levels, apart from the drop in cortisol with propofol. We observed a change in energy-producing metabolites.

Adult↗