PubMed Health⌕ Search

Biomedical subjects

F Pichinoty

Publications and source records attributed to F Pichinoty.

At least 19 recordsLinked to original sources
PubMed1990

[Isolation, morphological and biochemical characteristics of gram-positive bacteria metabolizing beta-caryophyllene].

Eleven strains of coryneform bacteria were isolated from soil samples by enrichment culture in a mineral medium containing beta-caryophyllene as the sole energy and carbon source. Ten of them could also metabolize longifolene. Numerical taxonomy, based on the use of 147 characteristics, revealed a large diversity. The DNA G + C content was found to be in the range of 62.5-68.3 mol%. Three strains were placed in "The National Collection of Industrial and Marine Bacteria".

Actinomycetales↗
PubMed1990

[Chemotaxonomy of gram-positive bacteria metabolizing beta-caryophyllene].

Chemotaxonomic identification of coryneform bacteria metabolizing b-caryophyllene was attempted. The following phospholipids were identified as main components of the bacterial extracts: cardiolipids, phosphatidylethanolamine, phosphatidylinositol and mannosides of phosphatidylinositol. Saponification of the lipid extracts gave a mixture of hydroxylated and nonhydroxylated fatty acids. Among the latter, oleic and tuberculostearic acids were identified. The hydroxylated fatty acids were analysed by thin-layer chromatography and mass spectrometry (as methyl esters). From the results thus obtained, the strains appeared to be more closely related to the genus Rhodococcus than to the genus Nocardia.

Chromatography, Gas↗
PubMed1979

[Carbon assimilation and taxonomic study of Bacillus subtillis and B. licheniformis].

All 14 strains of B. subtilis can use the following 17 sources of carbon and energy: D-glucose, D-mannose, D-glucosamine, salicin, D-ribose, maltose, sucrose, cellobiose, trehalose, arbutin, starch, mannitol, glycerol, glycerate, pyruvate, fumarate, and L-proline. All 15 strains of B. licheniformis can use the following 41 sources of carbon and energy: D-glucose, D-galactose, D-mannose, D-fructose, D-glucosamine, alpha-methyl-D-glucoside, beta-methyl-D-glucoside, salicin, D-gluconate, saccharate, D-xylose, L-arabinose, L-rhamnose, D-ribose, maltose, sucrose, cellobiose, melibiose, trehalose, arbutin, raffinose, starch, inulin, mannitol, D-sorbitol, glycerol, glycerate, citrate, L-malate, D-malate, mucate, pyruvate, fumarate, alpha-L-alanine, alpha-D-alanine, asparagine, L-glutamate, L-arginine, DL-ornithine, L-proline, and 4-amino-n-butyrate. The 29 strains form two distinct groups. Group A includes the 15 strains of B. licheniformis and 2 strains of B. subtilis; group B is formed of 11 strains of B. subtilis; the remaining strain of B. subtilis belongs to neither group. Bacillus licheniformis is a more homogeneous species than B. subtilis. The percentage of guanine + cytosine in the DNA of all 29 strains was determined. In the 14 strains of B. subtilis the average is 46.3% +/- 1.5. In the 15 strains of B. licheniformis the average is 46.4% +/- 0.9.

Bacillus↗
PubMed1978

[Isolation of bacteria that use that use nitric oxide as a respiratory electron acceptor under anaerobiosis].

Ten bacteria of the genus Bacillus were isolated from pasteurized soils, in anaerobiosis and at 32 degrees C, on peptone broth containing 0.5% KNO2. They are Gram variable rods producing oval spores. They are oxidase positive and have catalase. They grow, in anaerobiosis, on NO-3, NO-2, N2O, and NO as respiratory electron acceptors. These compounds are reduced to N2.

Anaerobiosis↗
PubMed1978

[Denitrification by Bacillus licheniformis].

The denitrifying capacity of 15 strains of Bacillus licheniformis was evaluated. In general, N2 production by the cultures on complex media containing NO3- is irregular and quite slow and three of the strains never produce gas. Bacillus licheniformis grows rapidly in anaerobiosis on peptone medium containing NO3- which is reduced to NO2-. None of the strains grow in peptone medium with NO2- or N2O as the respiratory substrate, nor do they grow under an atmosphere of 10% NO-90% N2. Denitrification was studied in cell suspensions using gas chromatography. N2O production from NO3- or NO2- is always weak at best; nitric oxide is reduced to N2O at an appreciable rate. All the strains synthesize nitrate reductase A in anaerobiosis when NO3- is present. In cell extracts, nitrite reductase activity is always negligible or nil with tetramethyl-p-phenylenediamine as an electron donor.

Anaerobiosis↗
PubMed1978

[Physiological study and taxonomy of Alcaligenes species: A denitrificans, A. odorans and A faecalis].

We have studied 43 strains of the species Alcaligenes dentrificans, A. odorans, and A. faecalis. Twenty-five of them were isolated by enrichment culture on minimal medium containing an organic acid (L-malate, succinate, tartrate, adipate, or itaconate) and N2O as a respiratory electron acceptor. These constitute a single phenon with the A. dentrificans strain type and 9 other strains isolated from clinical specimens. However, strain 4 differs from the other 34 strains in 12 nutritional characters, in its ability to effect a meta cleavage of diphenols, and by the absence of tetrathionate reductase. The percentages of G + C are the following: strains isolated from soil, 66.4 +/- 1.1; collection strains, 67.0 +/- 1.3. The 5 strains of A. odorans differ from the 34 strains of A. denitrificans (not including strain 4) in their inability to denitrify nitrate and use D-saccharate, adipate, pimelate, suberate, beta-hydroxy-beta-methylglutarate meso-tartrate, azelate, and itaconate. Their percentage of G + C is much lower: 56.1 +/- 0.4. From the nutritional point of view the 3 strains of A. faecalis resemble A. dentrificans. However, they differ from the latter by their inability to grow anaerobically on NO3-, NO2-, N2O, and by a slightly lower percentage of G+ C: 64.3 +/- 0.0. The 43 strains synthesize poly-beta-hydroxybutyric acid. None of them is chemolithotrophic.

Alcaligenes↗
PubMed1978

[Morphological, physiological and taxonomic studies of Bacillus azotoformans].

Seventeen strains of the new species Bacillus azotoformans were isolated by enrichment culture in peptone broth inoculated with pasteurized soil and then incubated under N2O at 32 degrees C. The bacterium is a Gram-negative rod, motile with peritrichous flagella, which produces oval spores without exosporia in swollen sporangia. However, the cells have thick walls, mesosomes, and persistent septa characteristic of Gram-positive bacteria. The bacterium lacks fermentative activity, does not attack carbohydrates, has complex growth requirements, and will grow anaerobically only if one of the following electron acceptors is present: NO3-, NO2-, N2O, S4O6--, or fumarate. Nitrate, nitrite, and nitrous oxide are denitrified with the production of N2. The microorganism is mesophilic, gives a positive oxidase reaction, synthesizes a type c cytochrome, and does not hydrolyse gelatin, starch, or "Tween 80." Poly-beta-hydroxybutyric acid is snythesized when the bacterium is grown in a medium containing DL-3-hydroxybutyrate. The following enzymes are present: nitrate reductase A, respiratory nitrite reductase, tetrathionate and fumarate reductases, and L-glutamate dehydrogenase. The following enzymes are absent: thiosulfate reductase, urease, lecithinase, arginine dihydrolase, phenylalanine deaminase, and catalase. For the 17 strains, the mean value of the G = C percent of the DNA is 39.8 +/- 1.2. All the strains are highly similar.

Anaerobiosis↗
PubMed1977

[Study of 14 denitrifying soil bacteria of the "pseudomonas stutzeri" group isolated by enrichment culture in the presence of nitrous oxide (author's transl)].

The strains were isolated from soil by enrichment in a liquid minimal medium containing ethanol, acetate, succinate, L-malate or tartrate, under an N2O atmosphere at 32 degrees C. All fourteen strains can use the following 25 sources of carbon and energy under aerobic conditions: glycerate, ethanol, propanol, acetate, butyrate, malonate, succinate, glutarate, sebacate, glycollate, L-lactate, D-lactate, L-malate, DL-3-hydroxybutyrate, pyruvate, fumarate, itaconate, mesaconate, crotonate, L-alpha-alanine, D-alpha-alanine, L-leucine, asparagine, L-tyrosine, and L-proline. They hydrolyze Tween 80 but not gelatin. Nitrate is used as nitrogen source. Nitrate reductase A and respiratory nitrite reductase are present. Four of the strains are clearly and easily distinguishable from the others on the basis of six characters: special morphology of colonies; in ability to use isovalerate and DL-valine, inability to use glucose, absence of exocellular amylase, and high level of metapyrocatechase. Their G + C content is 66-67%. One of the strains is distinct from the others by the yellow pigmentation of its colonies, its ability to use D-glucuronate, trehalose, D-sorbitol and citraconate, ability to grow at 4 degrees but not at 40 degrees, and a lower G + C content: 63%. One strain accumulates poly-beta-hydroxybutyrate. This work confirms the well-known, wide variability of the bacteria belonging to the P. stutzeri group. Denitrification by two of the strains was quantitatively studied using cell suspensions. Cells from NO-3-containing anaerobic cultures reduce NO-3, NO-2 and NO to N2O and N2; they reduce slowly N2O to N2. Cells grown in anaerobic cultures under N2O also reduce NO-3, NO-2 and NO to N2O and N2 but they reduce N2O rapidly to N2.

Culture Media↗
PubMed1976

The isolation and properties of a denitrifying bacterium of the genus Flavobacterium.

A previously undescribed denitrifying bacterium was isolated from soil. The cells are small gram-negative rods, asporogenous, and non-motile. Colonies become yellow after long exposure to light. This colouring is due to the production of a carotenoid pigment. The organism shows no fermenting activity, and grows only in the presence of one of the following electron acceptors: NO2, N2O, and O2. It does not reduce nitrate. It gives a positive oxidase test and has a cytochrome c and catalase. It requires no growth factors, is a chemoorganotroph and uses only sugars as carbon and energy supply. The DNA base composition is 40.8 moles percent GC. Although presenting the physiological characteristics of a pseudomonad, the organism described has been placed in the genus Flavobacterium because of its pigmentation and its low GC percentage.

Aerobiosis↗
PubMed1976

[A new, sporulating, denitrifying, mesophilic bacterium: Bacillus azotoformans N. SP. (author's transl)].

The described bacterium was isolated by enrichment culture in peptone broth inoculated with garden soil, pasteurized and then put to incubate under N2O at 32 degrees. It is a Gram-negative rod, motile with peritrichous flagella, and producing oval spores without exosporium in swollen sporangia. However, cells have the thick walls, mesosomes and persistant septa characteristic of Gram-positive bacteria. It lacks fermentative activity, does not attack carbohydrates, has complex growth requirements, and will grow anaerobically only if one of the following electron acceptors is present: NO3, NO2, N2O, S4O6, and fumarate. Nitrate, nitrite, and nitrous oxide are denitrified with production of N2. The microorganism is mesophilic, gives a positive oxidase reaction, synthesizes a type of c cytochrome, and does not hydrolyse gelatin, starch nor "Tween 80". The following enzymes are present: nitrate reductase A, respiratory nitrite reductase, tetrathionate and fumarate reductases, L-glutamate dehydrogenase, and superoxide dismutase. The following enzymes are absent: thiosulfate reductase, urease, lecithinase, arginine dihydrolase, L-alanine dehydrogenase, phenylalanine desaminase, and catalase. The GC% of its DNA is 39. The bacterium described can be considered to be a new species. We propose the name Bacillus azotoformans n. sp.

Bacillus↗
PubMed1975

[Denitrifying bacteria of genus Alcaligenes isolated from soil].

The organism isolated is a small non-sporulating Gram-negative rod, motile by means of peritrichous flagellae. It is an oxidase-positive chemo-organotroph utilizing O2,NO-3,NO-2 and N2O as resiratory substrates. Primary alcohols as well as numerous organic and amino acids are utilized as carbon and energy sources. Carbohydrates are not assimilated. Poly-beta-hydroxybutyrate is accumulated intracellularly. The bacterium is assigned to the genus Alcaligenes and its phenotype characteristics are compared with those of A. faecalis.

Alcaligenes↗