The effects of the antitumor drug bleomycin on polyploidizating hepatocytes of immature rabbits were investigated by combining cytochemical (static microfluorometry) and morphological (fluorescence and electron microscopy) approaches. In addition to a decelerating effect of the drug on the cell kinetics, there were several types of karyological damage (micronuclei, internuclear chromatin bridges, mitotic chromosome bridges), thus demonstrating a clastogenic activity of bleomycin on the DNA, Moreover, anomalous nuclear and cytoplasmic divisions suggest interference of the drug with other cellular structures (mainly the microtubule network) responsible for the cell kinetics.

Animals↗

PubMedDate not supplied

[Neonatal scrotal hemorrhage caused by post-traumatic hemoperitoneum].

The Authors describe a case of neonatal scrotal hematoma due to post-traumatic intra-abdominal hemorrhage. Differential diagnosis with testicular torsion and other causes of neonatal acute scrotum is considered and discussed.

Birth Injuries↗

PubMedDate not supplied

The action of cisplatinum (cis-DDP) on the postnatal growth of the rat liver. Cytokinetics and ultrastructural studies.

The early and late effects of cis-DDP treatment on liver cell kinetics were analyzed after its intraperitoneal injection into 17-day old rats. Frequency of binucleate hepatocytes, cellular DNA content distribution, 3H-thymidine labelling and ultrastructure of the nuclei were analyzed. Two days after treatment, a block of mononucleate hepatocytes in the S phase and in the G2 phase was demonstrated by the increase of intermediate 2c-4c and 4c DNA values in the absence of changes in 3H-thymidine labelling; 8c binucleate cells, which are essential for the formation of tetraploid mononucleate cells, were not found. In cell nuclei, large areas of more condensed chromatin appeared, perhaps providing further evidence for a G2 block. Seven days after treatment, there was a tendency to catch up with the normal situation, as shown by the unblocking of the S phase in mononucleate cells indicated by both cytophotometry and autoradiography. The presence of 8c binucleate cells and 4c-8c mononucleate cells indicates that 4c mononucleate hepatocytes are either diploid cells in the G2 phase or true tetraploid G1 cells. The decrease in the heterochromatin areas and the appearance of hypertrophic nucleoli demonstrate an increase in the metabolic activity of the nuclei. Thirty six days after treatment, the incidence of different DNA hepatocyte classes and the 3H-thymidine labelling were already similar in control and in treated rats.

Aging↗

PubMedDate not supplied

Cytochrome oxidase and lactate dehydrogenase activities in peroxidized rabbit epididymal spermatozoa.

We report a quantitative cytochemical study on cytochrome oxidase and lactate dehydrogenase activities on rabbit epididymal spermatozoa during spontaneous lipid peroxidation. Our data show that during aerobic incubation both in NTP and KTP media the sperm cytochrome oxidase activity undergoes a significant decrease. The lactate dehydrogenase activity shows different cytochemical patterns in comparison between the two media considered. Such activity significantly increases in rabbit spermatozoa suspended in NTP medium from the first until the sixteenth hour of incubation time. At the following times the lactate dehydrogenase activity significantly declines showing yet until the later times of incubation integrated optical density values fairly high. During the whole period of the aerobic incubation, the spermatozoa suspended in medium KTP show lactate dehydrogenase integrated optical density values which not significantly differ from those of the control in spite of an initial enhancement from the first until the thirteenth hour of the experimental treatment.

Animals↗

PubMedDate not supplied

[Spondylo-costal dysostosis. Description of a case and review of the literature].

The Authors describe a case of spondylocostal dysostosis. The infant, born from consanguineous Caucasian parents, showed at birth marked shortening of the trunk and neck. Radiographic examination revealed severe congenital anomalies of the entire spine, associated with secondary deformities of the ribs. A review of literature's cases, with differential diagnosis and pathogenetic hypothesis, is also reported.

Abnormalities, Multiple↗

PubMedDate not supplied

Enzymatic activities during the development and the involution of the yolk sac of the trout.

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Acid Phosphatase↗

PubMedDate not supplied

[Research on the quantitative limits of the Feulgen reaction. IV. Dry mass and intensity responses to the Feulgen reaction of fixed smears of nuclear material (author's transl)].

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Animals↗

PubMedDate not supplied

[HLA typing in a neonate with posttransfusional graft vs host disease (PT-GVHD)].

The authors report a case of post-transfusion-graft-versus-host disease (PT-GVHD) in a premature infant after parental blood donation. This disease seems to be due to the transfusion of immunocompetent T lymphocytes into an immunodeficient recipient or into an immunocompetent host who shares an HLA haplotype with HLA-homozygous blood donors (i.e. relatives or members of inbred populations) and who is therefore unable to reject the graft cells. The results of HLA typing of the patient and his family demonstrated that the infant was identical with his father for HLA class II antigens (DR, DQ) and, concerning HLA class I, he had in common the remaining paternal haplotype (A29, B44). Prevention of this disease, performed by gamma irradiation of blood components before transfusion, appears to be effective in most cases.

Blood Donors↗