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F Portaels

Publications and source records attributed to F Portaels.

At least 145 records · Page 8Linked to original sources
PubMed1986

A cooperative taxonomic study of mycobacteria isolated from armadillos infected with Mycobacterium leprae.

Seventeen strains of mycobacteria, recovered from six armadillos experimentally infected with Mycobacterium leprae, were examined in ten different laboratories. This collaborative study included use of conventional bacteriological tests, lipid analyses, determination of mycobactins and peptidoglycans, characterization by Py-MS, and immunological, metabolic, pathological and DNA studies. These armadillo-derived mycobacteria (ADM) formed five homogeneous groups (numbered ADM 1 to 5) on the basis of phenetic analyses. However, DNA studies revealed only four homogeneous groups since group ADM 1 and one of the two strains in group ADM 3 showed a high level of DNA relatedness. The phenetic and DNA studies confirmed that the ADM strains differed from all other known mycobacteria. Cultural, biochemical, metabolic and pathogenic properties as well as DNA-DNA hybridizations clearly differentiated these ADM from M. leprae.

Amino Acids↗
PubMed1985

Immunodiffusion analyses of environmental mycobacteria isolated in Zaire.

Unclassified, acid-fast strains isolated from the environment in Zaire were analysed by comparative immunodiffusion for taxonomical purposes. These strains have previously been shown to differ physiologically and/or biochemically from all other known mycobacteria. The analyses confirmed that these strains belong to genus Mycobacterium and showed, furthermore, that they were more closely related to the slowly growing mycobacteria, including Mycobacterium tuberculosis, than to the rapidly growing ones. The result of the serological analyses also confirmed a previous grouping of these unclassified strains. An antigen designated beta which, in all probability, is a ribonucleoprotein was shown in all but two of the test strains.

Corynebacterium↗
PubMed1985

Use of gas chromatography to differentiate Mycobacterium leprae from cultivable armadillo-derived mycobacteria, M. avium/intracellulare, and M. lepraemurium by analysis of secondary alcohols.

Two long-chain secondary alcohols, 2-octadecanol and 2-eicosanol, were demonstrated by gas chromatography in hydrolysates of Mycobacterium avium/intracellulare, in cultivable, armadillo-derived mycobacteria, and in M. lepraemurium grown in vivo, but they were not found in purified suspensions of M. leprae isolated from experimentally infected armadillos. Gas chromatographic analysis of these alcohols constitutes a method for rapid detection and quantification of contaminating mycobacteria in preparations of M. leprae intended, for example, for vaccine use. The technique may also be of value for critical evaluation of cultures of "in vitro-grown" M. leprae.

Animals↗
PubMed1983

Mycolic acid patterns of four vaccine strains of Mycobacterium bovis BCG.

Thin-layer chromatography of methanolysates of four widely used vaccine strains of Mycobacterium bovis BCG showed that only one organism had the expected pattern of mycolic acid methyl esters characteristic of Mycobacterium bovis and Mycobacterium tuberculosis. The remaining three BCG strains lacked methoxy mycolic acid.

BCG Vaccine↗
PubMed1982

Branched Mycobacterium-like organisms from swine lymph nodes.

Branched, weakly acid fast organisms were isolated from 5 of 112 caseous lymph nodes derived from slaughter-house pigs. In two cases they were associated with typical Mycobacterium avium strains. The bacteria differed from all other mycobacteria known at present. Limited experiments in pigs with one strain failed to disclose a possible pathogenic role in the host species although this strain was pathogenic for mice. Tests with this and three other strains showed that all four were apathogenic for chickens.

Abattoirs↗
PubMed1982

In vitro sensitivity of Mycobacterium lepraemurium for antimycobacterial drugs.

The sensitivity of Mycobacterium lepraemurium for isoniazide, dapsone, ethionamide, pyrazinamide, rifampicin, p-aminosalicylic acid, ethambutol and various sulfonamides was determined using Ogawa medium. The sensitivities of M. lepraemurium for these drugs are different from those obtained with M. leprae. Our results illustrate that M. lepraemurium is not a model for the drug sensitivities of M. leprae.

Anti-Bacterial Agents↗
PubMed1982

Sensitivity of mycobacteria to dapsone.

Dapsone sensitivity was tested on Loewenstein-Jensen medium on 164 mycobacterial strains belonging to 32 different species. A minimum inhibitory concentration as low as that of M. leprae, as determined "in vivo" was never observed. The most sensitive strains belong to the species M. kansasii, M. ulcerans, M. gordonae, M. szulgai and M. gastri with minimal inhibitory concentrations varying between 0.3 and 0.1 microgram/ml, still approximately 10 times the value known for wild strains of M. leprae.

Dapsone↗
PubMed1982

[A case of pulmonary mycobacteriosis due to Mycobacterium intracellulare (author's transl)].

An M. intracellulare, rough strain (serotype 7) has been isolated from sputum of a sixty-year old patients. This patient was a political prisoner in Germany between 1942-1945 and had contracted pulmonary tuberculosis with the cavity in the upper lobe of the right lung. A strain of mycobacterium susceptible to antituberculous drugs was isolated from his sputum in 1973. Since 1979, the isolation of M. Intracellulare has been accompanied by clinical signs of pulmonary mycobacteriosis, i.e. persistence of the cavity in spite of antituberculous treatments reapparition of cough with sputum, general weakness. Intradermo-reaction with specific sensitin gives a strong positive reaction, contrasting with a weak reaction with PPD tuberculin. As the antibiogram of the strain shows a susceptibility to cotrimoxazole and erythromycin, the patient underwent a therapy with the combination sulfamethaxozale-trimethoprime and erythromycin, for 4 months. At the end of this treatment, he seems to have recovered completely. The radiological aspect of the lungs remains unchanged. Although the source of contamination remains unknown, one thinks on the basis of bibliographica data, that it can be found in human beings, or seldom in animals (a pig, a bird) or in nature (a pond).

Animals↗
PubMed1981

Parameters influencing the in vitro growth of Mycobacterium lepraemurium.

Investigations were done on the role of several parameters of incubation, carbon sources, fatty acids and Tween 80 on the in vitro growth of M. lm on Ogawa medium. It was found that incubation of aerated tubes at 37 degree C in a humidified atmosphere was optimal. Glycerol is necessary in the medium at concentrations of 0.5 to 2%. Acetate inhibits growth; there is no beneficial effect from Tween 80 or sodium salts of oleic, caproic, caprylic and palmitic acid. The pH of the medium is very critical since optimal growth occurs only between pH 5.8 and 6.3 on Ogawa egg yolk medium.

Culture Media↗
PubMed1981

Activity of the combination of isoniazid, protionamide and dapsone against Mycobacterium leprae and some other mycobacteria.

No additive effect was found at the addition of isoniazid to either dapsone or protionamide against M. leprae and against highly dapsone-sensitive in vitro growing Mycobacterium sp. strains. It is concluded that the indication of the administration of isoniazid in leprosy is extremely limited and could only be envisaged as a companion drug in combined therapy of dapsone-resistant multibacillary leprosy in light-skinned people, when aminoglycosides cannot be administered.

Animals↗
PubMed1980

In vitro cultivation and characterization of Mycobacterium lepraemurium.

Three different strains of M. lm were regularly grown in vitro from suspensions of mouse organs if at least 10(5) organisms were inoculated on Ogawa egg yolk medium and incubated at 35 degrees C in a humidified, CO2 enriched atmosphere. Growth is slow and requires 2-3 months. Colonies are 1-2 mm in diameter, white to pale yellow. Microscopically the bacteria are acid-alcohol-fast pleomorphic rods with branchings and beaded filamentous forms. Mice inoculated with in vitro grown subcultures develop an infection indistinguishable from the one observed after injection with mouse passage strains of M. lm. The in vitro characteristics of the strains are identical and different from all other known mycobacteria.

Animals↗