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Biomedical subjects

F R Reale

Publications and source records attributed to F R Reale.

31 records · Page 2Linked to original sources
PubMed1991

Appendectomy in primary and secondary staging operations for ovarian malignancy.

Appendectomy was performed at primary or secondary staging operations in 100 patients with ovarian malignancies. Of 80 patients who underwent appendectomy at the time of their primary surgery, 25 (31.2%) had appendiceal metastases. Among 47 patients who were believed to have disease limited to the pelvis at the time of surgery--stage I (N = 34), II (N = 7), IIIA (N = 5), and those designated stage IIIC solely on the basis of microscopic para-aortic nodal metastasis (N = 1)--the appendix was involved with disease in only two patients (4.3%). However, among 33 patients with advanced disease--stage IIIB (N = 6), IIIC except those designated IIIC solely on the basis of microscopic paraaortic nodal metastasis (N = 19), and IV (N = 8)--the appendix was involved with disease in 23 patients (69.7%) (P less than .001). Poorly differentiated tumors and serous histologic cell types more frequently metastasized to the appendix (64, 15, 6, and 8% for grades 3, 2, and 1 and borderline histology, respectively; P less than .001; and 48, 13, and 8% for serous, endometrioid, and mucinous; P less than .001). Of 20 patients who underwent appendectomy at their secondary staging procedure, two had metastases. Metastatic disease in the appendix was microscopic in nine of 27 patients. Because the frequency of appendiceal metastasis is similar to that of other metastatic sites in stages I and II ovarian cancer, it should be removed at primary staging procedures. Appendectomy should also be performed in patients with advanced ovarian malignancies if it contributes to cytoreduction or at the time of secondary staging procedures.

Appendectomy↗
PubMed1990

Primary adenocarcinoma of the breast arising in the vulva.

Primary adenocarcinoma of ectopic breast tissue in the vulva has been reported in only four instances. A fifth case, in which estrogen and progesterone receptors were present, is described. Estrogen and progesterone receptor studies may be helpful in identifying adenocarcinoma of uncertain origin.

Adenocarcinoma↗
PubMed1990

Prognostic significance of estrogen and progesterone receptors in epithelial ovarian cancer.

Estrogen and progesterone receptor assays were analyzed from epithelial ovarian cancer in 123 patients to determine the prognostic significance of receptor results. In 110 (89%), assays were performed at diagnosis and in 13, assays were performed after previous therapy. Estrogen receptor determinations were positive (above 10 fmol/mg) in 75 cases (61%), progesterone receptor determinations were positive (above 3 fmol/mg) in 35 (28%), and both were positive in 20 (16%). Progesterone receptors were more frequently positive (53%) in tumors of endometrioid histology than with other histologic types (P = .01). Thirty-one subjects had a second assay; estrogen receptors were positive in 16 cases (52%), progesterone receptors were positive in five (17%), and both were positive in five (17%). Synchronous and metachronous assays were in agreement in 60-79% of the cases. By multivariate analysis, positive estrogen receptor, progesterone receptor, or both did not predict response to chemotherapy, negative second-look findings, or survival. Thirty-one women received hormonal therapy; one of 26 evaluable subjects had a partial response and four (13%) maintained stable disease for at least 6 months (6-21 months). Positive receptors did not predict hormonal response or disease stabilization.

Adult↗
PubMed1987

Characterization of a human malignant mesothelioma cell line (H-MESO-1): a biphasic solid and ascitic tumor model.

Human malignant mesothelioma of the pleura was successfully transplanted s.c. into athymic nude mice and grew as a solid neoplastic mass. Tumor growth resulted in death of the animals between 98 and 161 days after implantation. Minced samples of the growing tumor were propagated as a malignant peritoneal effusion. Animals with malignant ascites died predictably at 32 to 33 days. Light and electron microscopy, and immunocytochemistry demonstrated a similarity of the transplanted solid and fluid malignancies with the human primary mesothelioma. Cytogenetic analysis demonstrated a predominance of cells with a triploid number of identifiable but abnormal human chromosomes. This model, which mimics the clinical behavior of malignant mesothelioma in the human, may be of value in animal trials of chemotherapy and immunotherapy.

Animals↗
PubMed1979

Discriminant analysis on cells from developing squamous cancer of the respiratory tract.

Cytologic preparations made from the tracheobronchial tree taken by the Schreiber catheter have been scanned by three color microphotometry. The digitized cell images were processed by the analytical cytodiagnostic programs of the TICAS system. Cells were sorted into two control groups and five groups of increasing atypia ranging from normal epithelium to invasive squamous cell carcinoma. Standard statistical tests, including Wilk's Lambda, Rao's V, and the Kruskal-Wallis tests are performed on these subsets of cell image features. This study demonstrates that discriminant analyses permit differentiation between normal cells and those from marked atypia or carcinoma and that the classification achieves a high degree of agreement with visual assignment.

Animals↗
PubMed1979

Differentiation by TICAS analysis of cell populations of tracheal aspirates from hamsters with squamous-cell carcinoma.

Individual cells from the tracheal aspirates of hamsters exposed to benzo-a-pyrene were scanned at .5 mum in three colors. Features relating to size, shape, and color were extracted and calculated by computer. The single cells were then classified by these features into separate populations with varying degrees of atypia, extending up to frank cancer cells. A high degree of accuracy was attained in classification by these methods.

Animals↗
PubMed1979

A reciprocal translocation (X;11) in a female with gonadal dysgenesis.

A 24-year-old female patient was referred for evaluation of primary amenorrhea. Endocrine studies showed elevated gonadotropins, consistent with gonadal failure. At laparoscopy, a normal nulligravid uterus, normal fallopian tubes, and bilateral streak gonads were observed. Histologic studies showed that the left gonad consisted entirely of fibrous tissue, confirming the presence of streak gonads. Chromosome banding studies of peripheral blood and cultures of tissue from the left gonad demonstrated a 46,X,rcp(X;11)(q22;q13) karyotype. A review of reports of X-autosome reciprocal translocations indicated that abnormal gonadal development is associated with break-points in the mid-region of the long arm of the X chromosome.

Adult↗
PubMed1975

Isolation of type C virions from a normal human fibroblast strain.

Type C virions were spontaneously released from cultures of a diploid human cell strain. The varions have properties of known type C RNA tumor viruses and share antigenic determinants with the major interspecies-specific antigen (p30) of simian sarcoma virus. Antiserum to reverse transcriptase of gibbon ape leukemia virus inhibits the reverse transcriptase of the putative human virions and that of simian sarcoma virus, but has no effect on the corresponding enzymes of avian or murine RNA tumor viruses.

Antigens, Viral↗
PubMedDate not supplied

Torsion of the uterine adnexa: A clinicopathologic correlation.

Although uterine adnexal torsion is a rare phenomenon and an infrequent diagnosis, there have been several reported cases. Two cases that occurred in our practice in the past year are presented to illustrate the acute and chronic phases of the disease. This paper discusses the intrinsic and extrinsic concepts of etiologic factors of adnexal torsion and reviews the common presenting symptoms and the physical, laboratory and pathologic findings of this syndrome. An increased index of suspicion of intermittent adnexal torsion may lead to laparoscopic diagnosis and conservative therapy.

Adnexa Uteri↗
PubMedDate not supplied

An automated device for the production of cell preparations suitable for automatic assessment.

A set of preparatory steps has been developed for the processing of clinical samples from the female genital tract onto microscopic slides suitable both for machine assessment and for regular microscopic evaluation. These steps involve the depositing of the cell sample in a collection fluid, the semi-automatic adjustment of cell concentration to about 30,000 cells per milliliter through a combination of transmittance and light scatter measurements, the dispersion of the sample by syringing, and the deposition of the cellular content onto microscope slides by centrifugation. These steps were designed so as not to allow loss of cells--except the lysis of erythrocytes--with the understanding that this condition entails the presence of 4--10 per cent cell aggregates in the final preparation.

Autoanalysis↗
PubMedDate not supplied

Cytodiagnosis of Hodgkin's disease in sputum specimens.

In patients with histologically proven Hodgkin's disease, knowledge of the extent of involvement of lymph nodes and other organs has proven valuable in the determination of treatment and prognosis. One of the most common sites of involvement outside the hematopoietic system has been shown to be the lung parenchyma; in this study, six patients with a tissue-proven diagnosis of Hogdkin's disease and positive cytologic findings in the sputum were reviewed. Three cell types not found in normal sputum specimens were identified in these patients and were correlated with the histologic patterns of the tumors as seen in lung biopsies. Our results suggest the usefulness of sputum examination as an adjunctive or possibly a substitute diagnostic procedure in the evaluation of patients with Hodgkin's disease and possible lung involvement. They also suggest that in some cases the cytologic diagnosis can be quite specific in the identification of neoplastic cells as consistent with a diagnosis of Hodgkin's disease.

Adult↗