PubMed Health⌕ Search

Biomedical subjects

F R Seiler

Publications and source records attributed to F R Seiler.

119 records · Page 7Linked to original sources
PubMed1976

Development, production and quality control of clinically applied antilymphocyte globulin.

Various antigens have been assessed for the ability to produce anti-human lymphocyte globulin(ALG). In our hands the most satisfactory antigen has proved to be cultured lymphoblasts. These are used to produce Behringwerke ALG(Pressimmune). A brief outline of problems in the large scale production of ALG and how these are overcome is given. The quality control checks for activity and safety are discussed.

Animals↗
PubMed1975

Regression of spontaneous mammary tumors in dogs after injection of neuraminidase-treated tumor cells.

The effect on tumor progression produced by the injection of VCN-treated tumor cells in dogs with spontaneous mammary tumors was investigated. Untreated dogs of different races and different ages with at least two palpable spontaneous mammary tumors were selected. One of the tumors was left in the animal for further clinical examination whereas the other tumor(s) was (were) excised for preparation of a single-cell suspension by mechanical disintegration and enzymatic digestion with collagenase and trypsin. (1) In the first group, each animal was infected with 2 times 10-7 similarly prepared autologous, mitomycin-treated tumor cells; in 8 out of 12 dogs of this group the tumors progressed while so far 1 dog has died of metastasis. (2) In the second group, each animal received the same number of 2 times 10-7 tumor cells, which were mitomycin- and VCN-treated: 13 out of 15 dogs had a significant regression of their tumors to less than 10% of the original volume; in 1 dog the tumor remained unchanged and in 1 dog it progressed. (3) In the third group, 8 dogs received 1 times 10-8 mitomycin- and VCN-treated tumor cells: the application of this cell dose resulted in an accelerated tumor progression in all 8 dogs, 3 of which have already died of metastasis. The significance of these findings, with respect to potentiation and abrogation of the immunological response and with regard to immunotherapy in man, is discussed.

Animals↗
PubMedDate not supplied

Experimental Klebsiella septicemia in mice: treatment with specific antibodies from the rabbit alone and in combination with gentamicin.

Using a model of an experimental Klebsiella pneumoniae septicemia in mice, we examined the therapeutic effect of passively administered specific antibacterial antibodies from rabbits. Both specific IgM and IgG antibody proved to be therapeutically effective. However, the effect of IgG was markedly superior to that of IgM with regard both to the degree of protection and the time interval allowing efficient therapy after infection. The effect of IgG was due to a marked enhancement of in vivo phagocytosis, as demonstrated by monitoring bacterial numbers in the liver, spleen, lungs and kidneys. In mice immunocompromised with cyclophosphamide, treatment with IgG still exerted protection against low challenge inocula. When higher inocula were used, treatment with IgG ceased to influence the final mortality rates but delayed the course of the disease for several days by transient reduction of bacterial numbers in the parenchymal organs. In both normal and immunocompromised mice, concomitant treatment with gentamicin resulted in a marked synergistic enhancement of survival.

Animals↗