Biomedical subjects

F R Tay

Publications and source records attributed to F R Tay.

13 recordsLinked to original sources

PubMed1998

Relation between water content in acetone/alcohol-based primer and interfacial ultrastructure.

OBJECTIVES: This study investigated the ultrastructure of the resin-dentine interface when a two-bottle primer system containing NTG-GMA (N(p-tolyl)glycine-glycidyl methacrylate) and BPDM (biphenyl dimethacrylate) was used with different concentrations of water as a part of the primer solvent: (I) an experimental version of All-Bond 2 with no water in primer A; (II) a commercial version of All-Bond 2 (Bisco, Itasca, IL, USA) with 5% water in primer A; and (III) a former version of All-Bond 2 with 17% water in primer A. METHODS: Thirty-six 1-mm thick dentine discs prepared from third permanent molar teeth were each conditioned with 10% phosphoric acid for 20 s and rinsed for 20 s. They were randomly divided into three groups: Group A, conditioned dentine surface air-dried for 30 s; Group B, air-dried for 3 s; and Group C, blot-dried so that the dentine surface remained visibly moist. The three categories of primers were applied to each disc in 8-10 coats, resulting in nine sub-groups. Discs in each sub-group were bonded together to form disc-pairs using a chemical cure resin, demineralized in ethylene diamine tetraacetic acid (EDTA) and prepared for transmission electron microscopic examination. RESULTS: With the use of the water-free primer version, sub-optimal hybridization was observed whenever dentine was dried prior to bonding (Groups IA and IB). In the 5% water version, prolonged desiccation resulted in compromised hybridization (Group IIA), while resin globules were observed on the surface of the hybrid layer when a moist technique was employed (Group IIC). In the 17% water version, surface blisters and globules characteristic of the 'overwet phenomenon' were observed in Groups IIIB and IIIC. CONCLUSION: Between the two extremes of a morphological spectrum of bonding conditions, the different primer versions exhibited different sensitivity ranges. There was a shift in the 'window of opportunity' for optimal hybridization and tubular seal depending on the water content of the primer system investigated.

Acetone↗

PubMed1997

The overwet phenomenon in two-component acetone-based primers containing aryl amine and carboxylic acid monomers.

OBJECTIVES: The overwet phenomenon was first reported when a moist bonding technique was used with an earlier commercial version of All-Bond 2 (Bisco) that contained BPDM in primer B. This study investigated whether ultrastructural features of the overwet phenomenon could also be detected in other commercially available two-component acetone-based primers containing BPDM, PMDM and PMGDM, as well as an experimental two-component primer containing DSDM. METHODS: Thirty 1 mm dentin discs prepared from third molars were each conditioned with 10% H3PO4 for 20 s and rinsed for 20 s. They were randomly divided into 5 groups: Group I (Bond-It, Jeneric/Pentron:PMGDM); Group II (Wet Bond, Chameleon Dental Products:PMGDM); Group III (Tenure S, Den-Mat:PMDM); Group IV (present commercial version of All-Bond 2, Bisco:BPDM) and Group V (Experimental two-component primer system containing DSDM in primer B). Following a moist bonding technique using the respective system, discs from each group were further bonded together to form three disc pairs using a chemical-cured resin. Bonded disc pairs were demineralized in EDTA and processed for TEM examination. For this ultramicroscopical study, results such as the features of the overwet phenomenon were analyzed by visual inspection of the specimens in each group (n = 12). RESULTS: Isolated blister-like spaces of variable dimensions were observed within the primer layer in all groups and possessed the following characteristics: 1) a layer of resin-impregnated dentin was always present along the base of the primary blister; 2) surface primer globules, sometimes containing secondary blisters, were identified within these primary blisters; 3) dentinal tubules within the blister-like spaces were not completely sealed; 4) primer globules were circumscribed by a halo of fine kinked strands of material. SIGNIFICANCE: Although the technique of moist bonding is based on valid biological principles, incorporation of resin monomers that are immiscible with water rendered the application of current two-component, acetone-based primers very technique-sensitive in terms of tubular seal, when used on moist, acid-conditioned dentin. Further studies should be directed at elimination of this type of oil-in-water (O/W) "macroemulsion" formation through optimal micellar solubilization of these resin monomers in water.

Acetone↗

PubMed1997

Ultrastructure of the resin-dentin interface following reversible and irreversible rewetting.

PURPOSE: This study investigated the effect of reversible and irreversible rewetting on the application of One-Step, single-bottle, water-free, acetone-based primer/adhesive. MATERIALS AND METHODS: Thirty 1 mm dentin discs prepared from third molars were each conditioned with 10% H3PO4 for 20 seconds and rinsed for 20 seconds. They were randomly divided into five groups: Group 1 (positive control): conditioned dentin bonded visibly moist; Group 2: bonded after drying for 3 seconds; Group 3: drying for 3 seconds and rewetting with distilled water; Group 4 (negative control): drying for 3 seconds, stored in 8% glutaraldehyde for 15 minutes, rinsed with distilled water and dried for 3 seconds; and Group 5: drying for 3 seconds, stored in glutaraldehyde for 15 minutes, rinsed with distilled water and bonded visibly moist. One-Step was applied in two coats according to the manufacturer's recommendation and discs in each group were further bonded together to form a disc-pair using a chemical cure resin, demineralized in EDTA and prepared for TEM examination. RESULTS: Complete resin infiltration into the zone of demineralized dentin was observed in Group 1 and 3. In Group 2, resin infiltration was limited to the surface and base of the demineralized network apparently along the lateral branches of the dentin tubules. A middle zone of suboptimal infiltration was observed. In Group 4 and 5, minimal resin infiltration was only observed along the base of the demineralized network upon their fixation in a collapsed state with glutaraldehyde.

Collagen↗

PubMed1996

Micromorphological spectrum from overdrying to overwetting acid-conditioned dentin in water-free acetone-based, single-bottle primer/adhesives.

OBJECTIVES: The goal of this study was to illustrate and define the micromorphological spectrum which exists at the resin-dentin interface when two water-free, acetone-based, single-bottle primer/adhesive systems (One-Step, Bisco, and Prime & Bond, Dentsply/L.D. Caulk) were applied to acid-conditioned dentin under different dry and wet bonding conditions. METHODS: Forty-eight 1 mm dentin discs were each conditioned with 10% phosphoric acid and rinsed for 20 s. They were randomly divided into 4 groups based upon the status of the remaining surface moisture; Group I (30 s dry); Group II (3 s dry); Group III (blot dry) and Group IV (overwet). Bonded dentin disc pairs were then demineralized in EDTA and embedded in epoxy resin for transmission electron microscopic examination. RESULTS: The micromorphological spectra of the two bonding systems were essentially similar. Both were effected by even mild desiccation (3 s), resulting in incomplete intertubular resin infiltration. Optimal intertubular resin infiltration was achieved when the collagen network was kept moist and appeared relatively unaffected by the presence of excess surface moisture. On the other hand, intratubular resin infiltration was severely compromised in the presence of excess water within the dentinal tubules and at their openings in the dentin surface. The continuity of the resin layer deteriorated; blister-like spaces formed on the dentin surface and resin globules were found around the tubular orifices and on the surface of the hybrid layer. In addition, a complex phase separation pattern was observed in Prime & Bond that was characterized by phase inversion in the presence of surface moisture. SIGNIFICANCE: The "window of opportunity" for optimal interfacial integrity for both water-free systems appeared to depend upon keeping the demineralized collagen network moist, coupled with the complete evaporation of excess and "displaced" water from tissues prior to light-curing the prime/adhesives.

Acetone↗

PubMed1996

Resin permeation into acid-conditioned, moist, and dry dentin: a paradigm using water-free adhesive primers.

Preservation of the morphological integrity of demineralized dentin collagen in its hydrated state may account for the success observed in wet-bonding procedures. This study investigated the micromorphological differences between moist- and dry-bonding techniques with the use of: (a) Aelitebond, an alcohol-based, water-free, single-component dentin adhesive primer system; and (b) a water-free, acetone-based experimental primer similar to the acetone-based, water-containing All-Bond 2, a two-component primer system. In the wet groups, acid-conditioned dentin surfaces were blotted so that they remained visibly moist prior to bonding. In the dry groups, dentin surfaces were air-dried for 30 sec. Following the bonding procedures, dentin discs in each group were laminated together by means of a chemical-cure resin and processed for scanning electron microscope (SEM) and transmission electron microscope (TEM) examination. Conditioning with 10% H3PO4 for 20 sec. produced complete demineralization of the outer dentin. In the wet groups, banded collagen and interfibrillar spaces could be observed at the surface of the acid-conditioned dentin. Complete wetting of the loosely arranged collagen fibrils by the resin resulted in the formation of a hybrid layer. In the dry groups, only a very thin hybrid layer was observed on the dentin surface, along the walls of the tubules, and along the course of their lateral branches. The absence of banded collagen and interfibrillar spaces within these areas suggested the existence of a collapsed dentin matrix along various liquid-vapor boundaries that restricted resin permeation into the subsurface intertubular matrix, producing an incompletely infiltrated "hybridoid region".

Dental Bonding↗

PubMed1996

Quantitative contribution of the collagen network in dentin hybridization.

PURPOSE: To determine the quantitative contribution of dentin hybridization to bonded assembly strength and demonstrate the micromorphology of the interface with and without collagen present. MATERIALS AND METHODS: Four groups of 10 molar teeth were finished to a 320 grit dentin smear layer. Two groups served as controls and two experimental groups were subjected to collagenase digestion of the collagen exposed by acid conditioning. All-Bond 2 and Amalgambond were used to bond Bisfil and Epic resin composite, respectively. Stored in water at 37 degrees C for 24 hours the assemblies were tested in a shear mode at a crosshead speed of 5 mm/minute. Means and standard deviations were subjected to analysis for statistical significance. Twenty four teeth in four groups were examined by scanning (SEM) and transmission electron microscopy (TEM) for the relationship between resin and conditioned dentin with and without the collagen network. RESULTS: All-Bond 2 and Amalgambond controls were 28.41 +/- 3.9 and 19.04 +/- 5.96 MPa, collagenase-treated groups scored 26.43 +/- 2.90 and 19.70 +/- 4.25 MPa respectively. No significant difference existed between the control and experimental groups. SEM showed an intertubular collagen network with patent tubules and a pronounced porous, irregular dentin topography following collagen digestion. A distinct hybrid zone and tubular penetration was observed but the collagenase-treated specimens showed only resin in the tubules and their lateral extensions. TEM confirmed the absence of a distinct hybrid zone in the collagenase groups with a tight, gap-free junction between the resin and the undemineralized dentin. An electron dense zone (< 50 nm) at the leading edge of conditioning was observed for All-Bond 2 and Amalgambond groups. It was concluded that the resin-reinforced or hybridized, collagenous network does not detract from, nor contribute any significant quantitative value per se to dentin bonding with the systems tested.

Acid Etching, Dental↗

PubMed1996

The overwet phenomenon: a transmission electron microscopic study of surface moisture in the acid-conditioned, resin-dentin interface.

PURPOSE: To identify ultrastructural features resulting from possible primer-water interaction when a two-bottle, acetone- and alcohol-based dentin adhesive (All-Bond 2) was applied to acid-conditioned dentin in the presence of excess water. MATERIALS AND METHODS: Three groups (3-second dry, visibly moist, overwet), each comprising eight dentin discs prepared from human third molars, were established and bonded with All-Bond 2 under different degrees of surface moisture. Variations in the ultrastructural appearance of the resin-dentin interface from laboratory demineralized and epoxy resin embedded disc pairs were examined either with en bloc ruthenium red pre-staining alone or further stained with uranyl acetate and lead citrate. RESULTS: Displacement of water by the volatile primer solvents resulted in the formation of a layer of resin-impregnated dentin in the three groups. Interaction of the primer with water following the evaporation of the volatile primer solvents resulted in phase separation of the primer components. Ultrastructural features observed within the previously water-filled blister-like spaces on the dentin surface in the visibly moist and overwet groups recapitulated what was observed within partially water-filled dentin tubules in the 3-second dry group. The overwet phenomenon appeared to be a result of emulsion polymerization, involving irreversible interaction of water-soluble bifunctional primer components with those that are water-immiscible, through the formation of micelles, resulting in the absence of total interfacial integrity.

Acid Etching, Dental↗

PubMed1996

The overwet phenomenon: a scanning electron microscopic study of surface moisture in the acid-conditioned, resin-dentin interface.

PURPOSE: To study with the scanning electron microscope the interfacial phenomenon that occurred in the presence of excessive surface moisture following the application of an acetone-based adhesive to acid-conditioned dentin. MATERIALS AND METHODS: Specimens consisted of flat dentin discs that were divided into three groups based upon the status of remaining surface moisture: (1) 3-second dry group, (2) blot-dry group, with the conditioned dentin surface remaining shiny as opposed to matte, and (3) overwet group: an additional 40 mL of distilled water spread thin on the dentin surface after blot-drying. Polished resin-dentin interfaces were brought into relief using plasma-etching and examined without further embedding. RESULTS: Although a hybrid layer was observed in all three groups, there was (1) a sequential deterioration of the bonded assembly along the hybrid layer-resin interface, and (2) loss of complete tubular seal as the quantity of surface moisture increased. Intratubular resin globules were observed beneath short resin plugs in the 3-second dry group. Small, isolated blister-like spaces, trapped within the resin layer, were observed along the surface of the hybrid layer in the blot-dry group, while similar, but larger spaces were found in the overwet group. The blister-like spaces, which were often continuous with incompletely sealed tubular orifices, were partially filled with extraneous resin globules dispersed within an amorphous matrix.

Acid Etching, Dental↗

PubMed1996

The overwet phenomenon: an optical, micromorphological study of surface moisture in the acid-conditioned, resin-dentin interface.

PURPOSE: To evaluate the morphological effect of different degrees of surface wetness on "wet bonding" upon the acid-conditioned, resin-dentin interface using an acetone-containing adhesive system (All-Bond 2). MATERIALS AND METHODS: Dentin surfaces were conditioned with 10% phosphoric acid (20 seconds), washed and air dried (3 seconds) or blot dried. RESULTS: The surfaces were characterized by the formation of an intact layer of resin impregnated dentin. Dentin tubules were sealed with solid cores of primers commonly below which were intratubular primer globules. Excess moisture on the dentin surface resulted in the creation of blister-like spaces at the primer-dentin interface. A layer of resin-impregnated intertubular dentin was present and below such spaces were incompletely sealed tubules. Primer globules were also observed on the dentin surface. The presence of microscopic voids at the primer-dentin interface may offer a morphological explanation for the published decrease in bond strength when this system was applied in the presence of excessive surface moisture.

Acid Etching, Dental↗

PubMed1995

Comparison of three methods of critical evaluation of microleakage along restorative interfaces.

This in vitro study compared microleakage along the dentin-restorative interface by use of a conventional single-surface and two stereoscopical protocols. The extent of microleakage in class V cylindric cavities that were restored with Scotchbond 2/P-50 was assessed after thermocycling and silver staining were performed. Specimens were randomized into three groups of 10 teeth for the three different protocols to be investigated. The categories were group 1, a single longitudinal section through the midline of the restoration, group 2, a multiple-surface protocol based on computer reconstruction of digitized images from multiple serial abrasions, and group 3, a clearing protocol based on immersion of demineralized specimens in methyl salicylate. The severity of microleakage for all groups was recorded on an ordinal scale and based on linear leakage length. The median leakage score for groups 1 through 3 was 2, 4, and 4, respectively. When comparisons were performed with nonparametric statistical analysis, both the clearing and the multiple-surface protocols recorded significantly more severe leakage than those detected from a conventional, single longitudinal, midline sectioning technique, (x2 = 8.595, p < 0.02). Furthermore microleakage patterns generated from the two stereoscopical protocols revealed extensive microleakage that advanced unilaterally or bilaterally through the dentin-restorative interface along the embrasure regions into the axial wall of the restorations. No significant difference was noted between the two stereoscopical protocols (Q = 0.260, p > 0.05), which suggested that the clearing protocol, which is less labor-intensive than the multiple-surface protocol, may be recommended for future comparative microleakage studies that involve the dentin-restorative interface.

Analog-Digital Conversion↗

PubMed1995

Variability in microleakage observed in a total-etch wet-bonding technique under different handling conditions.

Using a stereoscopic clearing protocol and scanning electron microscopy, we investigated the extent and nature of microleakage in a total-etch, current-generation dentin adhesive by a wet-bonding technique under different handling conditions. The hypotheses were that inadequate light curing of the primer or incomplete drying of the primer solvent might adversely affect the sealing ability of an acetone-containing adhesive system. The study consisted of three experimental groups: (I) a control group with an adequate light source and with the primer solvent completely dried; (II) an "inadequate-light" group; and (III) an "incomplete evaporation of primer solvent" group. The extent of microleakage after silver staining and clearing of the specimens was scored based on a modified five-point scale. Nonparametric statistical analysis (Kruskal-Wallis ANOVA) followed by a multiple comparison test (Dunn test) indicated significant differences among the three groups (p < 0.05). SEM examination of the restorative interface revealed that microleakage appeared to be initiated from the bonding resin-hybrid layer interface in all three groups, representing the weak link in the adhesive system. In addition, microleakage was characterized by 5 zones, each delineating a stage in a continuous array of progressively deleterious microleakage patterns variously distributed among the three groups. It was suggested that, while the bonding resin-hybrid layer interface represented the intrinsic weakness in an already much improved dentin adhesive, extrinsic factors such as the adequacy of the curing light and, more importantly, complete removal of the primer solvents can and should be avoided to preserve the structural integrity of the marginal seal.

Acid Etching, Dental↗

PubMed1995

A method for microleakage evaluation along the dentin/restorative interface.

PURPOSE: To present a simple non-abrasive protocol in conjunction with the silver-staining method, enabling unobstructed visualization of well-defined microleakage patterns within the demineralized dentin-restorative interface. MATERIALS AND METHODS: Class V preparations were made with an occlusal margin on bevelled enamel and a gingival margin on dentin/cementum. After restoration with All Bond 2/P-50 and Scotchbond 2/Silux, storage in water at 37 degrees C for 7 days and thermocycling 300 times between 5 degrees and 55 degrees C, the specimens were placed in silver nitrate solution. After silver fixation, demineralization and clearing in methyl salicylate, silver stain location was scored ordinally. In addition, a three dimensional portrait of leakage pathways was illustrated using photographic and computer digitization. RESULTS: The Wilcoxon signed-rank test showed that the extent of silver penetration before and after acid demineralization was not significantly different (P > 0.05). This study also confirmed that microleakage is not uniform along the circumference of a restorative margin. Although the protocol is insensitive to microleakage occurring solely within enamel, the predominant concern over that occurring within dentin, in particular along the cervical restorative border, renders it a serviceable alternative for future investigation of the integrity of the marginal seal from clinically restored in vivo specimens.

Acid Etching, Dental↗

PubMed1994

Structural evidence of a sealed tissue interface with a total-etch wet-bonding technique in vivo.

The resin-dentin interface of in vivo specimens restored with the All-Bond 2 system by use of a total-etch wet-bonding technique on vital deep human dentin was investigated by: (a) SEM examination of cryofractured specimens; (b) SEM examination of acid-rinsed, highly polished, embedded specimens; and (c) TEM examination of demineralized ultrathin sections. Ultrastructural features that were pertinent to the formation of an effective clinical seal were characterized. It is suggested that the establishment of an effective seal of the patent dentinal tubules following total etching is accomplished by: (1) the formation of an outer zone of a solid resin plug surrounded by a circumferential cuff of resin-impregnated dentin; and (2) the formation of an inner zone of a hollow resin sheath with resin globules along the internal walls of the tubules, closely adapting to the odontoblast process. The structural appearance and functional implications of these two zones were strikingly similar, with the sealing of exposed dentinal tubules in arrested carious lesions or cervical abfraction lesions. It appeared that there is a common denominator in physiologic isolation defense mechanisms and the total-etch, wet-bonding technique in the All-Bond 2 system in preserving the biological and morphological integrity of the pulpo-dentinal complex.

Acid Etching, Dental↗