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Biomedical subjects

F Rodríguez

Publications and source records attributed to F Rodríguez.

At least 127 records · Page 7Linked to original sources
PubMed1997

Presence and distribution of 5HT-, VIP-, NPY-, and SP-immunoreactive structures in adult mouse lung.

A large number of biologically active substances have been identified and characterised in the respiratory tract of several mammals. These substances (amines and peptides) exert important regulatory influences on respiratory functions, and they act as neurotransmitters/neuromodulators, both being released from nerve terminals as neuroendocrine cells. However, these substances can also have other effects which suggest a paracrine action. Thus, to understand the role of amines and peptides in the lung, it is important to explore their localisation in different species. By using immunocytochemical staining methods we have studied the morphology and distribution of serotonin-, Substance P-, neuropeptide Y- and VIP-like immunoreactivity in the adult mouse lung. Moreover a pretreatment with colchicine, pargyline and 5-hydroxytryptophan as staining enlargement method was made. A widespread distribution of isolated endocrine cells and neuro-epithelial bodies containing 5HT-like immunoreactivity was recorded within the lung. NPY-like immunoreactive nerve fibres were localised in the airway smooth muscle and surrounding the blood vessels. VIP-like immunoreactivity was revealed in single cells as well as in some nerve fibres and ganglia around the blood vessels and in the bronchial smooth muscle. SP-like IR was observed in nerve fibres located in the smooth muscle of the airways, surrounding bronchi and bronchioli but not next to the intrapulmonary blood vessels. Their localisation both in cells and nerve fibres of the respiratory system suggests that they play a role in the regulatory function of the mouse respiratory tract, exerting their influence by endocrine, paracrine, neurosecretory pathways or a combination of all of these.

Animals↗
PubMed1997

Tracheal malformation in a bicephalic Honduran milk snake (Lampropeltis hondurensis) and subsequent fatal Salmonella arizonae infection.

A bicephalic Honduran milk snake (Lampropeltis hondurensis) with tracheal duplication and malformation and Salmonella arizonae infection is described. There were atypically wide collapsed tracheal rings with necrotizing tracheitis and abundant necrotic epithelial debris and inflammatory cells obstructing the lumen in one of the duplicate tracheae. Salmonella arizonae was cultured from the malformed duplicate trachea and was considered to be the etiologic agent causing necrosis.

Animals↗
PubMed1997

[The use of high-frequency jet ventilation for ventilatory support in an extreme case].

We report the case of a 27-year old man given a left lateral thoracotomy for emergency repair of the aortic isthmus, which had partially ruptured as a consequence of chest trauma. The patient also suffered serious trauma to the right lung, such that selective ventilation of that lung had to be applied with the left lung collapsed to allow the surgeon access to the descending aorta. Rapid decrease in SpO2 occurred, with excessively high airway pressures, requiring reinstatement of conventional ventilation of both lungs and interruption of surgery. Conventional ventilation was then replaced by high frequency jet ventilation to both lungs. That technique, combined with intermittent inflation of the left lung (inclined), provided sufficient oxygen as well as a wide and sufficiently immobile surgical field.

Adult↗
PubMed1997

Downmodulation of HLA class I expression by dexamethasone in MCF-7 cell line.

Several reports have shown the importance of MHC class I antigens in enabling the host to regulate tumour growth in vivo. Glucocorticoid hormones have strong immunosuppressive effects and are known to be regulators of gene transcription. In this report the expression of Major Histocompatibility Complex (MHC) class I antigens in six breast carcinoma cell lines have been studied before and after treatment with the synthetic glucocorticoid dexamethasone. Hence, HLA class I expression in the MCF-7 cell line was down-regulated in the presence of dexamethasone. This down-modulation of expression appeared to be mediated by transcriptional mechanisms, as revealed by HLA-class I mRNA levels.

Breast Neoplasms↗
PubMed1996

African swine fever: morphopathology of a viral haemorrhagic disease.

Twelve miniature pigs were infected experimentally with the moderately-virulent Spanish isolate of African swine fever virus E75. The disease was reproduced acutely and subacutely, and six days after infection the animals showed severe clinical and pathological signs characterised by diffuse haemorrhages in lymphoid and non-lymphoid organs. An immunohistopathological study, using two different methods, and a monoclonal antibody (mAb) (18BG3) were used to analyse the distribution of the virus protein 73 (VP73) and IgM and IgG. VP73 was observed primarily in cells of the mononuclear phagocyte system, and in many megakaryocytes, some tubular epithelial cells of the kidneys, tonsillar epithelium, some hepatocytes, and in a few endothelial cells and neutrophils in the later stages of the infection. The histological findings and the distribution of VP73 and IgM and IgG indicated that the virus caused haemorrhagic lesions through its effect on various haemostatic mechanisms.

African Swine Fever↗
PubMed1996

Functional and immunological properties of the baculovirus-expressed hemagglutinin of African swine fever virus.

A recombinant baculovirus harboring the hemagglutinin (HA) gene of African swine fever virus, with homology to the T-lymphocyte surface antigen CD2, was constructed. The efficient expression of the HA gene was determined by immunofluorescence and Western blot studies on insect cells infected with the recombinant baculovirus. The baculovirus-expressed HA showed hemadsorption and erythrocyte-agglutinating activities characteristic of the CD2 homolog protein induced by the virus in infected macrophages. Pigs immunized with the recombinant HA developed hemagglutination-inhibition and temporary infection-inhibition antibodies that recognize a 75-kDa structural protein and were protected against lethal infection.

African Swine Fever↗
PubMed1996

Renal biopsy findings and followup of renal function in rheumatoid arthritis patients treated with cyclosporin A. An update from the International Kidney Biopsy Registry.

OBJECTIVE: To review data from the International Kidney Biopsy Registry, which describes the occurrence of cyclosporin A (CSA)-induced nephropathy, and to discuss the potential risk factors for its development. METHODS: The report examines data on a total of 60 first and 14 second renal biopsies performed in rheumatoid arthritis (RA) patients treated with CSA for up to 87 months. RESULTS: Five of the 60 patients with RA included in the Biopsy Registry had findings consistent with CSA-induced nephropathy at first biopsy. One further patient had such findings at second biopsy. Of the 22 patients who started CSA at dosages < 4 mg/kg/day and subsequently received dosages no higher than 5 mg/kg/ day, none developed CSA-induced nephropathy. Continuous assessment of renal function did not show any evidence of deterioration over time in patients maintained on low-dose CSA. CONCLUSION: The data indicate that in RA patients being treated according to current dosing recommendations, the risk of developing CSA-induced nephropathy is low.

Adolescent↗
PubMed1996

Telencephalic ablation in goldfish impairs performance in a 'spatial constancy' problem but not in a cued one.

This work was aimed to study if goldfish telencephalon is involved differentially in spatial and cue learning. With this purpose, animals were assigned to two learning conditions, 'spatial constancy' and 'directly cued', and their performance was recorded before and after ablation of the telencephalon. During the presurgical acquisition period, animals of both groups learned to solve the task with accuracy, and reached the goal in transfer tests, even though they were released from new starting positions and the response requirements were changed. Ablation impaired selectively the solution of the spatial constancy problem, but had no significant effects on the cued condition. However, with additional training, performance of the ablated animals in the spatial constancy condition improved to control levels. The above data suggest that fishes can implement multiple spatial learning strategies which have different neural substrata. These results are discussed in relation to the possible nature of the representation underlying each task.

Animals↗
PubMed1996

Are there any clinical signs and symptoms that are related to endometriosis in infertile women?

OBJECTIVE: Our purpose was to assess the physical signs and clinical symptoms associated with endometriosis in infertile women. STUDY DESIGN: This case-control study was carried out in an academic tertiary hospital. There were 174 infertile women with endometriosis and 174 infertile women without endometriosis, all of them studied by laparoscopy. Before laparoscopy a standard interview and a standard physical examination were performed. RESULTS: Cul-de-sac nodularity was more frequent in infertile women with endometriosis than in infertile women without endometriosis (6.3% vs 0%). Although uterosacral tenderness was also more frequent in infertile women with endometriosis (7.5% vs 1.7%), uterosacral tenderness without nodularity was similar in both populations. Uterine retroversion and cul-de-sac obstruction frequencies were somewhat higher in the endometriosis group (p<0.10). The remaining signs and symptoms analyzed, including pelvic pain and dysmenorrhea, were similarly frequent in both populations. Symptoms were similarly frequent in all American Fertility Society stages, although adnexal mass was higher in stage IV. CONCLUSION: Uterosacral nodularity was pathognomonic of endometriosis in infertile women. Uterosacral nodularity and uterosacral tenderness (associated with uterosacral nodularity) were the only symptoms or signs of value to indicate endometriosis in infertile patients. The remaining clinical signs, as well as clinical symptoms, were of no value in diagnosing endometriosis in infertile women.

Biopsy↗
PubMed1996

A pathological and immunohistochemical study of caprine pleuropneumonia induced by subspecies of Mycoplasma mycoides.

In a population of 700 goats, 150 died; of these, 29 were necropsied. Ten of the 29 goats had pleuropneumonia. Mycoplasma mycoides subsp. mycoides (Large Colony) (MmmLC), Mycoplasma mycoides subsp. capri (Mmc), and Pasteurella multocida were isolated from five of the pleuropneumonic goats. Gross and microscopical lesions were typical of caprine pleuropneumonia (CPP), with bronchopneumonia, fibrinopurulent or fibrinonecrotic pleuropneumonia and dilatation of the interlobular septa and pleura. Immunohistochemical examination with antisera against MmmLC and Mmc showed mycoplasma antigens in all 10 goats with CPP. In all cases, both MmmLC and Mmc antigens were detected together. Mycoplasma antigens were present in the lumina of the airways and alveoli, mainly inside the cytoplasm of neutrophils and macrophages, but extracellular antigen was demonstrated in areas of necrosis. Pasteurella antigens were detected in four of the 10 animals with CPP. From the histological, immunohistochemical and microbiological results it was concluded that the two mycoplasmas, acting together, caused the pleuropneumonia, with P. multocida playing a subsidiary synergistic role.

Animals↗
PubMed1996

Immunohistopathological study of African swine fever (strain E-75)-infected bone marrow.

A study was made of the action of African swine fever virus (ASFV) on the bone marrow of 12 miniature pigs inoculated intramuscularly with the moderately virulent ASFV isolate E75 and killed 2 to 12 days after infection. A sequential description is provided of the histological lesions of the bone marrow in the experimental animals, which developed haemorrhagic lesions from 6 days after inoculation onwards. Immunohistochemical techniques were used to demonstrate the viral protein VP73 and immunoglobulins (IgG and IgM) in formalin-fixed and paraffin wax-embedded samples of bone marrow tissue. The immunohistological results, platelet counts, viraemia, and anti-ASFV immunoglobulin titres all indicated that thrombocytopoiesis impairment by direct viral action plays a role in the progressive thrombocytopenia characteristic of infection by moderately virulent ASFV isolates.

African Swine Fever↗
PubMed1996

Pathological and immunohistochemical studies of natural and experimental Mycoplasma bovis pneumonia in calves.

Lung tissues from calves naturally and experimentally infected with Mycoplasma bovis were subjected to histopathological and immunohistochemical examination. The latter was carried out with a monoclonal antibody raised against M. bovis, and an avidin-biotin-peroxidase complex (ABC) detection substrate system. Pulmonary lesions in naturally infected calves included exudative bronchopneumonia and extensive foci of coagulative necrosis surrounded by inflammatory cells. Experimentally infected lungs showed suppurative bronchiolitis and varying degrees of peribronchiolar mononuclear cell cuffing. M. bovis antigen in field cases was mainly detected at the periphery of the areas of coagulative necrosis, in necrotic exudates, and in close association with infiltrating macrophages and neutrophils. In lung tissue from calves with induced M. bovis pneumonia, antigen was located in epithelial cells, within inflammatory cells in airway lumina, and in alveolar walls. Other microbiological observations suggested that the ability of M. bovis to invade and cause lung parenchymal damage could be influenced by the participation of other pathogens.

Animals↗