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F Roux

Publications and source records attributed to F Roux.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

In vitro plasma binding of some second generation antitumor platinum complexes.

The kinetics of five cisplatin analogs binding to human plasma fractions possessing a molecular weight greater than 50,000 daltons were studied. Each drug solution in plasma ultrafiltrate or phosphate buffer (pH = 7.4, mu = 0.154) was mixed with human plasma and filterable platinum concentrations were measured versus time by atomic absorption spectrophotometry. The only Pt IV compound studied did not bind. All the other Pt II complexes bound, but their binding kinetics were quite different. The experimental data were fitted to a theoretical equation based on the hypothesis that plasma nucleophilic agents possessing a molecular weight greater than 50,000 daltons are able to react with Pt compounds with an apparent second order rate constant. The apparent reaction rate constants and initial concentrations of these nucleophilic agents were calculated. The difference between the respective values obtained for each cisplatin analog could be explained by differences in their chemical formulas. Therefore our result should contribute towards a better understanding of the pharmacokinetics of the cisplatin analogs studied.

Antineoplastic Agents↗
PubMedDate not supplied

Characterization of transferrin receptor in an immortalized cell line of rat brain endothelial cells, RBE4.

The content and distribution of transferrin receptors in an immortalized cell line, RBE4, derived from rat cerebral capillary endothelial cells was investigated using the monoclonal antibody MRC OX-26 (OX-26 mAb) specific for the rat transferrin receptor. An ELISA assay was developed with which the OX-26 mAb can be determined quantiatively. The detection limit of the assay was 10 pg or 0.07 fmol of murine antibody. With this technique accurate measurement of native antibody is now possible without the need for isotope labeling (iodination). Immunostaining of confluent monolayers of RBE4 cells using an antibody directed against the tight junction associated protein ZO-1 was indicative for structural intactness of RBE4 cell monolayers. OX-26 immunostaining demonstrated localization of the transferrin receptor at the plasma membrane and/or in the cytosol. Binding studies showed saturation of OX-26 mAb binding. The antibody binding analysis gave a dissociation constant (KD) of 17.1 +/- 1.2 nmol/l. The total amount of transferrin receptors present per cell was 70,800 +/- 17,000. Our results indicate that receptor binding of OX-26 mAb can be studied using an in vitro cell culture model of rat brain mircrovessel endothelium in conjunction with an ELISA technique for detection of native antibody. This approach will be used to investigate mechanisms of transendothelial transport of OX-26 in vitro.

Animals↗
PubMedDate not supplied

[Toxic action of lindane. Biochemical and ultrastructural changes in the lysosome system in cultured hepatocytes].

Lindane, in contact with cultured foetal chicken liver cells over a period of 40 hours, has a biphasic action on the lysosomal system of the cells. For the concentrations between 1 micronM and 25 micronM not affecting the cellular growth, the decrease of the acid phosphatase activity and the absence of any reaction in the Golgi saccules might indicate a reduction in the synthesis of the lysosomal enzyme. For the concentrations above 25 micronM slowing down cellular growth, acid phosphatase activity is partialrogeneous content and an increase in the relative acid phosphatase activity in the soluble extralysosomal fraction, for doses over 150 micronM, might indicate the development of the phenomenon of autophagocytosis.

Acid Phosphatase↗