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Biomedical subjects

F Rubio

Publications and source records attributed to F Rubio.

69 records · Page 4Linked to original sources
PubMed1980

[Detection and identification of irregular anti-erythrocyte antibodies with the Groupamatic G 360. Adaptation to the routine].

The screening of irregular red-cell antibodies on 20.200 recipient samples was carried out simultaneously on continuous flux AUTO-ANALYZER (LIP-bromeline) and GROUPAMATIC (TPC-bromeline). From results it appears: --an equivalent sensibility of both technologies to detect Rh, Kell, Duffy and Kidd antibodies; --a higher sensibility of Groupamatic to detect particularly Lewis and P1 antibodies; --a clear superiority of TPC compared to bromeline technique which is commonly used with Groupamatic. Small modifications of the standard TPC system allow to associate, in routine work, detection of irregular red-cell antibodies with other analyses, such as ABO Rh grouping and phenotyping. From this last point of view, a restricted test of coagglutination phenotyping has revealed to be very promising.

Antibodies↗
PubMed1979

Pharmacokinetics of cefamandole in patients undergoing hemodialysis.

The pharmacokinetics of Cefamandole was studied in 17 patients with terminal renal impairment, 10 of which were undergoing sessions of hemodialysis while 7 were in the period between dialysis sessions. An open two-compartment kinetic model was used to describe the bi-phasic decrease of the plasma concentrations of Cefamandole thus establishing the amounts of the antibiotic in the peripheral and central compartments together with the amount eliminated. All patients received an i.v. bolus injections of 15 mg/kg body weight. During the hemodialysis sessions, the pharmacokinetic parameters of Cefamandole were the following: alpha = 5.006 hr-1 beta = 0.182 hr-1 K12 = 2.598 hr-1 K21 = 2.147 hr-1 K13 = 0.441 hr-1 Vc = 5.700 l Vp = 6.190 l Vdss = 11.94 l It may be seen that there is a decrease in the overall elimination constant compared with that obtained during the periods between the dialysis sessions. A dosage regimen of multiple doses is established as a function of the pharmacokinetic parameters of the antibiotic for patients with terminal renal impairment undergoing periodic sessions of hemodialysis.

Adolescent↗
PubMed1977

Spectrofluorometric determination of substituted tetrahydrocarbazoles by a methylene blue sensitized photolytic reaction.

A spectrofluorometric method was developed for the determination of 6-chloro-9-[2-(2-methyl-5-pyridyl)ethyl]-1,2,3,4-tetrahydrocarbazole-2-methanol hydrochloride and its carboxylic acid analog in blood and urine. It involves extraction of both compounds at neutral pH, either from blood into ethyl acetate (the residue of which is dissolved in either) or from urine directly into ether. Both the alcohol and the acid are separated from each other by selective extraction into acid or base, respectively, and then reextracted into either from the respective aqueous medium by appropriate pH adjustment. The residues of the ether extracts containing the compounds are dissolved separately in 0.25 N NH4OH. Methylene blue is added to all samples, which are then exposed to UV energy for 15 min to produce the fluorophores. The fluorescence of the solutions is read at 370 nm, with excitation at 340 nm. The linear range of quantitation of both compounds is 0.02-10 mug/each/ml of final solution. The method was applied to the determination of blood levels and urinary excretion of the alcohol and its acid metabolite in a dog.

Animals↗
PubMedDate not supplied

Cerebral infarction of the basal ganglia due to embolism from the heart.

We studied 8 patients with cerebral infarction in the deep territory of the middle cerebral artery (MCA). All patients had a definite cardiac source of emboli and no known factors for thrombosis. Mixed sensory and motor deficit was found in all but one patient and CT scan showed larger lesions than usually reported in lacunar infarcts. Contrast enhancement was seen in all cases in which CT scan was performed in the second or third week. It is concluded that embolic infarcts in deep cerebral territory of MCA from a cardiac source are more frequent than previously reported. This diagnosis has to be considered when CT scan demonstrates a deep cerebral infarct.

Adult↗
PubMedDate not supplied

Balint's syndrome. Report of four cases with watershed parieto-occipital lesions from vertebrobasilar ischemia or systemic hypotension.

Four patients with Balint's syndrome secondary to bilateral parieto-occipital ischemic lesions are presented. The pathogenesis of these lesions corresponded to either occlusive arterial disease in the vertebro-basilar system or the effects of systemic hypotension. The clinical features of this syndrome are discussed, along with the pathophysiology and time course of the condition, with particular emphasis on etiological factors, which make of this condition a distinct clinico-pathological syndrome.

Adolescent↗
PubMedDate not supplied

Disposition of [14C]acitretin in humans following oral administration.

The disposition of the antipsoriatic agent, acitretin, was investigated in six healthy human volunteers who each received a single, oral 50 mg dose of [14C]acitretin (50 microCi). plasma, urine, and feces were collected for 240 hr after administration. Mean values of 20.9 and 62.6% of the administered dose were recovered in the urine and feces, respectively. The terminal elimination half-life of total radioactivity from the plasma was approximately 120 hr. Extraction of pooled plasma samples followed by separation by HPLC and quantitation by liquid scintillation counting indicated that acitretin and its 13-cis-isomer, isoacitretin, were minor fractions of the total drug-related material in the plasma at most time points up to 72-hr postdose. The structures of acitretin, isoacitretin, and two other metabolites--(5E,7E)-8-(4-methoxy,2,3,6-trimethylphenyl)-2,6 -dimethyl-5,7- octadienoic acid (I) and (5E,7E)-8-(4-hydroxy-2,3,6-trimethylphenyl)-2,6-dimethyl-5,7 -octadienoic acid (II)--were confirmed by MS and cochromatography with authentic standards. I and II were major fractions of the drug-related material in the plasma at all time points. Other compounds, whose structures could not be confirmed, also account for a significant fraction of the circulating radioactivity.

Acitretin↗
PubMedDate not supplied

[Human leukocyte antigen and otosclerosis].

Fifty patients (32 women, 18 men) who underwent stapedectomy [FISCH, V. (1), 1982] for otosclerosis were studied. Histo-compatibility antigens were typed in all patients using the microlymphocytic test to determine antigens of the HLA system loci A and B. Statistical comparison of results with those obtained in a control group of 339 persons without otosclerosis showed that otosclerosis has a genetic HLA-related component.

Chromosomes, Human, Pair 6↗