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Biomedical subjects

F S Sutherland

Publications and source records attributed to F S Sutherland.

4 recordsLinked to original sources
PubMed1990

Retinal oxygen consumption in vitro. The effect of diabetes mellitus, oxygen and glucose.

In diabetes mellitus, the retina is exposed to fluctuations in blood glucose concentrations, presumed decreases in oxygen availability and other metabolic disturbances induced by the disease. These metabolic phenomena may play a role in diabetic retinopathy. To understand these processes better, we measured oxygen consumption in vitro in retinas from normal and diabetic rabbits and examined the effect of oxygen and glucose concentration on retinal respiration. Retinal oxygen consumption is decreased in diabetic retinas compared to normal rabbit retinas (0.56 +/- 0.09 ml of oxygen per min per mg dry weight in normal and 0.40 +/- 0.07 ml of oxygen per min per mg dry weight in diabetic retinas). Retinal oxygen consumption is elevated if the oxygen tension is raised above normal in both groups. Glucose concentration did not significantly effect the oxygen consumption of the retina in either group.

Animals↗
PubMed1987

Experimental diabetes in cats induced by partial pancreatectomy alone or combined with local injection of alloxan.

Experimental diabetes was produced in cats by partial pancreatectomy using a short and technically simple surgical procedure. Electrocautery was used to cauterize pancreatic blood vessels and seal free edges of remaining pancreatic tissue to prevent secretion of pancreatic enzymes into the peritoneal cavity. In a second group of animals, partial pancreatectomy was followed by local injection of alloxan into an arterial branch of the cranio-mesenteric artery. The combined procedure resulted in diabetes mellitus in 100% (8 of 8) animals as compared to only 70% (14 of 20) in those subjected to partial pancreatectomy alone. In addition, the alloxan-pancreatectomized cats had a reduced latency period prior to onset of chronic hyperglycemia (4.8 days compared to 19.3 days postoperatively in pancreatectomized cats). The diabetic cats were maintained in poor metabolic control (blood glucose approximately 300 mg/dl) by daily injections of low doses of long-acting insulin. Pancreatic enzyme supplementation was given by mouth. Weight changes and blood glucose levels were monitored carefully to maintain the health of the animals while keeping them in poor metabolic control.

Alloxan↗
PubMed1986

Panretinal photocoagulation and retinal oxygenation in normal and diabetic cats.

We measured preretinal oxygen tension over laser-treated and intact retina in normal and diabetic cats. The oxygen tension was significantly higher over the area given panretinal photocoagulation than over the intact retina when the cats breathed 100% oxygen and the same trend was seen when the cats breathed 21% oxygen. There was no difference in preretinal oxygen tension between the normal and diabetic retinas. Light and electron microscopy showed laser damage to the mitochondria-rich photoreceptors in the outer retina as well as the retinal pigment epithelium. The inner retina showed no photocoagulation damage. Because panretinal photocoagulation destroys parts of the outer retina, thus reducing its oxygen consumption and allowing oxygen to diffuse from the choroid into the inner retina, it improves the oxygen supply to the inner retina.

Animals↗