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F S Young

Publications and source records attributed to F S Young.

7 recordsLinked to original sources
PubMed1981

Regulation of the synthesis of E. coli elongation factor Tu.

Rapidly growing E. coli with two active genes (tufA, tufB) for elongation factor (EF) Tu contains three times as much EF-Tu (tuf) mRNA as EF-G (fus) mRNA on a molar basis, but about seven times as much EF-Tu as EF-G or ribosomes. The concentration and translational efficiency of fus (EF-G) mRNA is about that for ribosomal protein mRNAs. The high molar concentration of EF-Tu relative to EF-G or ribosomes is achieved in part by translating tuf mRNA more efficiently than these other mRNAs. In a tufA+ tufB-: : Mu strain, the tuf:fus mRNA ratio is 1, but the concentration of EF-Tu and tuf mRNA is the same as in the wild-type strain. Thus cells with only an active tufA gene increase the concentration but not the translational efficiency of tuf mRNA. In such cells the concentration of fus mRNA is almost three times that in the wild-type strain. Because the tufA gene is distal to but cotranscribed with the fus gene as part of the four gene str operon, the wild-type concentration of tuf mRNA in these tufB- cells must be produced by increasing the concentration of transcript corresponding to the entire str operon. Thus transcription of the tufA gene can only proceed from the str promoter. Extracts of the tufB- cells contain tuf transcripts that correspond not only in size to the entire 4.5 kb str operon, but also to the size (approximately 1 kb) of a tuf gene. Our evidence suggests that this 1 kb tuf transcript is derived by posttranscriptional modification of the primary str operon transcript and that this modification could in part explain the high translational efficiency of tuf mRNA.

Bacterial Proteins↗
PubMed1978

Effect of inhibitors of elongation factor Tu on the metabolic regulation of protein synthesis in Escherichia coli.

By using inhibitors of elongation factor Tu (L-1-tosylamido-2-phenylethyl chloromethyl ketone [TPCK] and kirromycin), we determined the effect of elongation factor Tu inhibition on the synthesis of individual components of the translation machinery. The rates of synthesis of individual proteins were measured in double-label experiments using a two-dimensional gel system. TPCK inhibition produce a coordinate decrease in the differential synthesis rates of all components of the translation machinery examined in these experiments. On the other hand, kirromycin inhibition increased the differential synthesis rates of some translation components and decreased the differential synthesis rates of others. These results suggest that the metabolic regulation of synthesis of various translation proteins is not mediated through a common signal.

Amino Acid Chloromethyl Ketones↗