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Biomedical subjects

F Sági

Publications and source records attributed to F Sági.

3 recordsLinked to original sources
PubMed1997

Factors affecting transient expression of vector constructs in wheat protoplasts.

Direct uptake of reporter gene constructs with the bacterial beta-glucuronidase (GUS) gene fused to various promoters was achieved to embryogenic cell suspension culture-derived protoplasts of GK Ságvári winter wheat (Triticum aestivum L.) with polyethylene glycol (PEG) treatment. Based on GUS specific activity values, it was found that Mg2+ with PEG at 20% final concentration can significantly increase the transient expression in wheat protoplasts in comparison to the Ca(2+)-containing medium. The optimum incubation time in transformation mixture was 5-10 min at 25 degrees C. Transient GUS expression as detected by spectrofluorimetry was positively correlated with the time elapsed after DNA uptake (with maximum activity at 48 h), and the incubation time in GUS reaction mixture. It was also found that the protoplast culture medium plays an important role in the efficiency, and the treated wheat protoplasts cultured in KM medium showed a higher GUS activity than those kept in GM medium. Among the five plasmid constructs 6-16-fold higher promoter activity has been achieved with pKM794 driven by CaMV 35S promoter plus two enhancer elements than with the other constructs tested.

Gene Expression↗
PubMed1993

High-efficiency plant regeneration from an embryogenic cell suspension culture of winter wheat (Triticum aestivum L.).

Highly embryogenic cell suspension cultures were established from immature embryo-derived embryogenic calli of winter wheat (Triticum aestivum L., cv. GK Ságvári). Weekly subcultures were made in liquid MS medium supplemented with 2 mg 1(-1) 2,4-D. An average of twenty-two compact, organized calli were obtained from each 1 ml suspension cells when plated on solid MS medium containing IAA and zeatin under a 16/8 h light/dark cycle, while only 9 calli were produced in the dark. Variation in the callus inducing ability was correlated to the time elapsed after subculture. Plated cells responded best 9 days after the subculture and 59% of the calli were regenerable, retaining their embryogenic capacity over 6 months. Several hundred green shoots and plants were regenerated on differentiation media supplemented with IAA and BAP, and 300 plants were transferred to the greenhouse. The majority of plants had an abnormal chromosome number and a low viability.

Cells, Cultured↗