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F Salas

Publications and source records attributed to F Salas.

10 recordsLinked to original sources
PubMed2006

Are taxonomic distinctness measures compliant to other ecological indicators in assessing ecological status?

Assessing the ecological status, a concept implemented in the European Water Framework Directive [EC, 2000. Directive of the European Parliament and of the Council 2000/60/EC establishing a framework for community action in the field of water policy PE-CONS 3639/1/00, p. 72], requires the application of methods capable of distinguishing different levels of ecological quality. The Average Taxonomic Distinctness has been used as tool in this context, and we tested the robustness of Taxonomic Distinctness measures applying it in different scenarios (estuarine eutrophication, organic pollution, and re-colonisation after physical disturbance), analysing simultaneously its compliance to other types of ecological indicators. Results show that, in most of the case studies, only Total Taxonomic Distinctness was relatively satisfactory in discriminating between disturbed situations. Other Taxonomic Distinctness measures have not proved to be more sensitive than other ecological indicators (Shannon-Wiener, Margalef, and Eco-Exergy indices). Therefore, this approach does not seem to be particularly helpful in assessing systems' ecological status with regard to the WFD implementation.

Animals↗
PubMed2005

Are taxonomic distinctness measures compliant to other ecological indicators in assessing ecological status?

Assessing the ecological status, a concept implemented in the European Water Framework Directive [Directive of the European Parliament and of the Council 2000/60/EC establishing a framework for community action in the field of water policy PE-CONS 3639/1/00, 72 p.], requires the application of methods capable of distinguishing different levels of ecological quality. Somerfield and Clarke [Marine Environmental Research 43 (2003) 145-156] proposed Average Taxonomic Distinctness to be used as tool in this context. We tested the robustness of Taxonomic Distinctness measures applying it in different scenarios (estuarine eutrophication, organic pollution, and re-colonisation after physical disturbance), analysing simultaneously its compliance to other types of ecological indicators. Results show that, in most of the case studies, only Total Taxonomic Distinctness was relatively satisfactory in discriminating between disturbed situations. Other Taxonomic Distinctness measures have not proved to be more sensitive than other ecological indicators (Shannon-Wiener, Margalef, and Eco-Exergy indices). Therefore, this approach does not seem to be particularly helpful in assessing systems' ecological status with regard to the WFD implementation.

Animals↗
PubMed1999

EpoDB: a prototype database for the analysis of genes expressed during vertebrate erythropoiesis.

EpoDB is a database of genes expressed in vertebrate red blood cells. It is also a prototype for the creation of cell and tissue-specific databases from multiple external sources. The information in EpoDB obtained from GenBank, SWISS-PROT, Transfac, TRRD and GERD is curated to provide high quality data for sequence analysis aimed at understanding gene regulation during erythropoiesis. New protocols have been developed for data integration and updating entries. Using a BLAST-based algorithm, we have grouped GenBank entries representing the same gene together. This sequence similarity protocol was also used to identify new entries to be included in EpoDB. We have recently implemented our database in Sybase (relational tables) in addition to SICStus Prolog to provide us with greater flexibility in asking complex queries that utilize information from multiple sources. New additions to the public web site (http://www.cbil.upenn.edu/epodb) for accessing EpoDB are the ability to retrieve groups of entries representing different variants of the same gene and to retrieve gene expression data. The BLAST query has been enhanced by incorporating BLASTView, an interactive and graphical display of BLAST results. We have also enhanced the queries for retrieving sequence from specified genes by the addition of MEME, a motif discovery tool, to the integrated analysis tools which include CLUSTALW and TESS.

Animals↗
PubMed1998

EpoDB: a database of genes expressed during vertebrate erythropoiesis.

EpoDB is a database designed for the study of gene regulation during differentiation and development of vertebrate red blood cells. In building EpoDB, we have taken the in advance approach to the data integration problem: we have extracted data relevant to red blood cells from GenBank, SWISS-PROT, TRRD (transcriptional regulation data) and GERD (expression levels data) to create a single integrated, highly curated view. Tools have been developed to automate data extraction from online resources, cleanse data of errors, enter information manually from the primary literature, generate a uniform, canonical representation of information and maintain data currency. The database is organized around biological features, e.g., genes, rather than sequences, which are supported by a controlled and consistent vocabulary for gene names and gene family names. Beyond the standard database queries, the functionality of EpoDB includes the ability to extract features and subsequences, display sequences and features graphically using bioWidget viewers and integrated analysis tools. EpoDB may be accessed at: http://cbil.humgen.upenn.edu/epodb/

Animals↗
PubMed1997

[The prediction of the degree of metabolic control by minimal technical norms in a diabetes program].

OBJECTIVE: To assess whether scores on the minimum technical norms (MTN) of the diabetes mellitus programme are of use in determining the level of metabolic control achieved. DESIGN: A retrospective study. SETTING: Two teaching health centres in the city of Santander. PATIENTS AND OTHER PARTICIPANTS: 204 patients, the total chosen by the Santander area management for the 1994 annual evaluation of the Diabetes programme. MEASUREMENTS AND RESULTS: The values reached by the variables of the MTN were measured. For a control criterion we took the value of glycosylated haemoglobin HbA1C or over. 52% of patients fulfilled the criteria of good control, 20% acceptable and 28% bad. CONCLUSIONS: Scoring on the MTN has little value in predicting lower values of HbA1C and is no use in assessing the level of metabolic control achieved by the Diabetes programme. The evaluation of the programme should include results indicators.

Community Health Centers↗
PubMed1995

Functional expression of falcipain, a Plasmodium falciparum cysteine proteinase, supports its role as a malarial hemoglobinase.

Erythrocytic malaria parasites degrade hemoglobin as a principal source of amino acids for parasite protein synthesis. We have previously shown that a Plasmodium falciparum trophozoite cysteine proteinase, now termed falcipain, is required for hemoglobin degradation, and we have hypothesized that this proteinase is responsible for initial cleavages of hemoglobin. To further evaluate the biological role of falcipain, we expressed the enzyme in bacterial and viral expression systems. After expression in the baculovirus system, falcipain was enzymatically active and had biochemical properties very similar to those of the native proteinase. Recombinant falcipain rapidly hydrolyzed both denatured and native hemoglobin. Hemoglobin hydrolysis was blocked by cysteine proteinase inhibitors but not by inhibitors of other classes of proteinases. Our results support our hypothesis that falcipain is a critical malarial hemoglobinase that is responsible for both initial cleavages of hemoglobin and the subsequent hydrolysis of globin into small peptides.

Animals↗
PubMed1991

Epstein Barr virus/complement C3d receptor is an interferon alpha receptor.

Interferon alpha contains a sequence motif similar to the complement receptor type two (CR2/CD21) binding site on complement fragment C3d. Antibodies against a peptide with the CR2 binding sequence on C3d react with a peptide carrying the IFN alpha CR2 binding motif (residues 92-99) and with recombinant IFN alpha. The IFN alpha-derived peptide, as well as recombinant IFN alpha, inhibits C3bi/C3d interaction with CR2 on the Burkitt lymphoma Raji. The direct interaction of IFN alpha and CR2 is inhibited by polyclonal anti-IFN alpha, anti-CR2 and anti-C3d peptide antibodies as well as by C3bi/C3d, EBV coat protein gp350/220 and IFN but not by IFN gamma. [125I]IFN alpha binding to Raji cells is inhibited by polyclonal anti-IFN alpha and anti-CR2 antibodies, by peptides with the CR2 binding motif and partially by C3bi/C3d. Monoclonal anti-CR2 antibody HB5, but not OKB-7, blocks IFN alpha binding to Raji cells. CR2 or CR2-like molecules may therefore be the major IFN alpha receptors on B lymphocytes.

Amino Acid Sequence↗
PubMed1988

Transcripts of the Drosophila blastoderm-specific locus, terminus, are concentrated posteriorly and encode a potential DNA-binding finger.

The commitment of cells to specific fates, as well as the transitions in the cell cycle and transcription that occur at the cellular blastoderm stage of Drosophila embryogenesis, suggest that there are genes with unique functions expressed specifically at this stage. In an attempt to identify such genes, we used molecular screening to isolate several loci which encode blastoderm-specific transcripts (Roark et al., (1985). Dev. Biol. 109, 476-488). We report here the complete nucleotide sequence of one of these genes, terminus (ter), which maps to 75C1,2. The predicted ter protein possesses a transcription factor IIIA (TFIIIA)-like putative Zn-binding, DNA-binding finger. The ter RNA, detected by in situ hybridization, is distributed uniformly in the embryo during the syncytial blastoderm stage, and then becomes more concentrated in the posterior during the late cellular blastoderm stage. During gastrulation, the RNA is most concentrated in the amnioproctodeal invagination; it is also found at a lower concentration in the ventral furrow and in the anterodorsal neurogenic region. By the end of germ band extension, ter RNA is no longer detected.

Amino Acid Sequence↗
PubMedDate not supplied

Intermittent continuous iv infusion of high-dose cyclophosphamide for remission induction in acute lymphocytic leukemia.

Cyclophosphamide was administered to 42 patients with acute lymphocytic leukemia (ALL) as a daily continuous iv infusion at a dose of 400 mg/m2/day x 5 days; the courses of treatment were repeated every 3 weeks. Of the 42 patients entered, 21 achieved a complete response, two achieved a partial response, 12 failed to respond, and seven were considered to have early deaths. The response rate was 69.5% if only patients who received adequate trials are considered; mean duration of response was 18.5 weeks and mean survival time was 24.2 weeks. Twenty-three patients had relapsed after previous chemotherapy, and 19 patients were untreated for advanced high-risk cases of ALL; no difference was found in the response rates, durations of response, and survival times between these groups. No significant genitourinary toxicity occurred. Myelosuppression became the dose-limiting toxic effect. Continuous infusion of cyclophosphamide is a clinically effective method of ALL treatment and may have a role in the initial combination regimens for this hematologic malignancy.

Adolescent↗