Biomedical subjects

F Sedor

Publications and source records attributed to F Sedor.

4 recordsLinked to original sources

PubMed1994

Stability of plasma lactate in vitro in the presence of antiglycolytic agents.

The use of plasma lactate to assess metabolic or circulatory impairment requires definition of critical preanalytical and analytical parameters. Stability has been documented for only 15 min after acquisition when samples were collected with fluoride and transported on ice. We examined time elapsed before analysis, storage temperature, and the antiglycolytic agent used to define preanalytical conditions. Plasma lactate was measured with a Kodak Ektachem 700XR analyzer. In controlled studies on volunteers, storage on ice slowed but did not eliminate the production of lactate; for samples collected with sodium fluoride (F) and potassium oxalate (OX), lactate increased by 0.2 mmol/L after 1 h, then changed little regardless of the storage temperature. For patients' samples collected in F/OX, the mean increase was only 0.15 mmol/L after 24 h. Samples with leukocytosis (neutrophil counts 23 x 10(9)-52 x 10(9)/L) were also stable, with a mean increase of 0.3 mmol/L at 8 h. Use of the antiglycolytic agents F and OX (at 60 and 12 mmol/L, respectively) maintained apparently stable lactate concentrations at room temperature for up to 8 h without special handling.

Acidosis, Lactic↗

PubMed1984

Breast milk iodide: reassessment in the 1980s.

In addition to iodized salt, the American diet is inadvertently supplemented with iodine from other foods, such as bread and cow's milk. The iodine content of breast milk has not been assessed recently in this country. Sixty-one samples of breast milk from 37 women were analyzed for iodide content using an iodide-selective electrode method. Mean breast milk iodide level on initial samples for this population was 178 micrograms/L (range 29 to 490 micrograms/L), about four times the recommended daily allowance for infants. Iodide levels in breast milk did not correlate with the age of the infant (ie, stage of lactation). There was a significant correlation between the iodide level in the breast milk and dietary iodine as estimated by a food frequency questionnaire, and iodized salt intake was significantly related to the iodide content of the breast milk.

Adult↗

PubMed1981

Unpredictable fluctuations in serum acid phosphatase activity in prostatic cancer.

Repeated determinations of the elevated serum acid phosphatase activities in five patients with advanced prostatic cancer were found to be highly variable during 24 to 48 hours of observation. Samples collected every three hours had fluctuations of 44% to 97% around the 24- to 48-hour mean values. These fluctuations appeared to be random, had no apparent circadian rhythm, and were unrelated to concurrent medications or activity. These spontaneous variations indicate the need for caution when using serial serum acid phosphatase determinations as an indicator of the response of prostate cancer to therapy. Elevated serum alkaline phosphatase activities did not show these extreme fluctuations.

Acid Phosphatase↗

PubMedDate not supplied

Nomogram for estimating creatinine clearance.

Several methods have been published for estimating creatinine clearance from serum creatinine concentrations. Such estimates of creatinine clearance are widely used for dosage adjustments of drugs which are primarily eliminated through the kidneys in patients with reduced renal function. Most of these methods involve the use of equations, requiring a few steps of calculations. A simple and easy-to-use nomogram is presented for estimating creatinine clearance from serum creatinine concentration, plus the age, sex, and bodyweight of the individual patient. This nomogram is based on the linear relationship between creatinine clearance and the reciprocal value of the serum creatinine concentration, where the slope of this relationship is determined by the rate of creatinine production. The rate of creatinine production, however, is related to age, sex, and bodyweight. These physical characteristics are therefore used to scale the slopes of the relationships between creatinine clearance and serum creatinine concentration. The validity of the nomogram was tested in 50 consecutive hospitalised patients for which creatinine clearance was measured. There was an excellent correlation (r = 0.903) between predicted and observed creatinine clearance values.

Creatinine↗