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Biomedical subjects

F Smadja-Joffe

Publications and source records attributed to F Smadja-Joffe.

44 records · Page 3Linked to original sources
PubMed1980

Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice.

Myeloproliferative virus, derived from Moloney sarcoma virus, causes erythroleukemia and myeloid leukemia in adult mice. This virus is also capable of fibroblast transformation in vitro. The virus consists of two separable biological entities which have been cloned. The helper virus component caused no visible changes in adult mice, whereas the defective virus induced both spleen focus formation and a large increase in erythroid precursor cells but retained the sarcoma virus property of transforming fibroblasts in vitro. Thus, myeloproliferative virus is the first murine sarcoma virus which induces erythroleukemia in adult animals.

Animals↗
PubMed1977

Induction in rats of paranasal sinus carcinomas with radioactive cerium chloride.

A colloidal suspension of radioactive cerium stabilized in the form of hydroxide was inoculated into young Sprague-Dawley rats near the maxillary sinus. Well-differentiated epidermoid carcinomas of the paranasal sinus were obtained in 8 of 12 rats and in 8 of 9 animals that liver more then 200 days. Therefore, this istope was highly effectivein inducing tumors at the site of injection. The uniformity of the histologic type of tumors induced under these experimental conditions was remarkable.

Animals↗
PubMed1976

Study of cell death in Friend leukaemia.

Cell death of splenic Friend leukaemic cells has been studied in vivo, using 125I-UdR and 3H-TdR pulse labelling. The evolution of the splenic specific activity has been measured by autoradiography and external counting during 40 hr after injection of the labelled precursor. These two techniques show the existence of a large reutilization of 3H-TdR (50%), which is measurable as soon as 7 hr after the injection. The DNA turnover rate is rapid, 83-8% of the splenic cellular DNA being renewed per day. Those results confirm that most of the cells produced in the Friend leukaemic spleen are rapidly lost; they also demonstrate that this cell loss is mainly due to a massive death, which occurs in proerythroblastic and erythroblastic compartments after one or two cell divisions. Friend leukaemic cells, which are characterized by a limited capacity of proliferation and a short lifespan, do not appear to be malignant.

Animals↗
PubMedDate not supplied

Induction of erythropoiesis with erythrocyte production in bone marrow cell culture "in vitro".

In the system of long-term bone-marrow culture described by Dexter et al., the differentiation of hematopoietic stem cells into erythropoietic cells is not observed. In this system, bone marrow are grown in a medium supplemented with horse serum. We describe here the induction of erythrocytic maturation in the bone-marrow culture by the addition of 5% fetal calf serum, one or two weeks after seeding the bone-marrow cells. We obtained the persistance up to 5 weeks of erythrocytic production. This phenomenon does not depend on the presence of erythropoietin in fetal calf serum.

Animals↗