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Biomedical subjects

F Solomon

Publications and source records attributed to F Solomon.

47 records · Page 3Linked to original sources
PubMed1978

A case of listeriosis in pregnancy with fetal survival.

Listeriosis during pregnancy is generally considered to result in an intrauterine infection which affects the fetus before birth or in the perinatal period. A case of listeriosis in a 38-yr-old diabetic woman with an influenza-like disease in her 32nd wk of pregnancy is presented. Two successive blood cultures were positive for Listeria monocytogenes type 4b and her serum showed a rising titer for agglutinins to this organism. After antibiotic treatment of the patient she gave birth to a healthy child.

Adult↗
PubMed1977

Binding sites for calcium on tubulin.

Calcium ions can inhibit the in vitro assembly of microtubules and, therefore, may play a role in the regulation of microtubule formation in vivo. In order to test the validity of this hypothesis; the interaction between calcium and pruified brain microtubular protein has been investigated by standard binding assays. We have detected and characterized two classes of binding sites for calcium on tubulin, the major component of cytoplasmic microtubules. There is a single high-affinity site per tubulin molecule, characterized by a dissociation constant of 3.2 X 10(-6) M. That site is inhibited by magnesium (k1 = 5 X 10(-5) M) and potassium chloride. There are approximately 16 low-affinity sites which have a dissociation constant of 2.8 X 10(-4) M, and which are also inhibited by potassium chloride. Binding at the low-affinity sites is slightly enhanced by low magnesium concentrations. Both classes of sites are distinguishable from the colchicine binding site, and are apparently also distinct from the vinblastine and guanine nucleotide sites. The characteristics of the calcium binding activity of tubulin are similar to those found for the calcium-binding proteins of sarcoplasmic reticulum. The results are consistent with a physiological role for calcium in the regulation of microtubule assembly.

Animals↗
PubMed1976

Utilization of the inactivation rate of coenzyme A transferase by thiol reagents to determine properties of the enzyme-CoA intermediate.

The rate of inactivation of succinyl-CoA:3-ketoacid coenzyme A transferase by thiol reagents is increased 3 to 100 times by very low concentrations of acyl-CoA substrates. The same maximum inactivation rate is found with acetoacetyl-CoA and succinyl-CoA. The enhanced rate of inactivation is caused by the stoichiometric formation of the enzyme-CoA intermediate and an accompanying conformation change of the enzyme. The inactivation rate provides a simple assay for the amount of enzyme present as the enzyme-CoA intermediate, using only catalytic concentrations of enzyme. This technique has been utilized to measure (a) a rate constant for hydrolysis of the enzyme-CoA intermediate of 0.10 min-1 at pH 8.1; (b) a stoichiometry of two active sites per enzyme molecule; and (c) the equilibrium constants for formation of the enzyme-CoA intermediate from dilute solutions of substrates (and hence for the overall reaction) by determining the ratio of [enzyme-CoA]/[enzyme] in the presence of a series of substrate "buffers" at different ratios of [RCOO-]/[RCOSCoA]. As the total concentration of acyl-CoA and carbosylate substrates is increased, the inactivation rate is decreased. This indicates that the Michaelis complexes are protected against inactivation.

Binding Sites↗
PubMed1976

Intermolecular tritium transfer in the transcarboxylase reaction.

Transfer of tritium from [3-3H]pyruvate into propionyl-CoA is found during the reaction of transcarboxylase: Methylmalonyl-CoA + pyruvate leads to oxalacetate + propionyl-CoA. About 5% of the tritium counts that are labilized in the reaction are found in a position of the propionate that exchanges rapidly with water in the presence of transcarboxylase. Transfer from [2-3H]propionate of propionyl-CoA to pyruvate is real but only about one-tenth as great. The tritium transfers between reactants on two subunits are difficult to explain by a "carbanion" mechanism of --C--H bond cleavage and support the cyclic mechanism in which carboxybiotin itself is the base and the enol form of biotin is the proton-transferring agent.

Carboxyl and Carbamoyl Transferases↗
PubMed1976

Isolation of Mycoplasma from the placenta after cesarean section.

Placentas obtained at cesarean section were cultured for Mycoplasma and other microorganisms in 123 randomly selected patients in order to evaluate the incidence of Mycoplasma, to identify factors which may contribute to their presence, and to correlate their presence with the occurrence of postpartum infection. Twenty-eight placentas (22.8%) yielded Mycoplasma positive cultures. The incidence of Mycoplasma in the placenta was significantly higher in patients with ruptured membranes. The incidence of postpartum fever was significantly higher (P less than 0.01) in cases positive for Mycoplasma as compared to cases in which the placenta was negative for Mycoplasma. Findings were similar for both groups with regard to the incidence of unexplained postpartum fever. The results of this study suggest that Mycoplasma may be considered a relatively frequent pathogen and should be considered a possible cause of postpartum fever.

Cesarean Section↗
PubMed1975

Infections with mycoplasma and bacteria in induced midtrimester abortion and fetal loss.

Placentas and fetuses from cases of induced midtrimester abortion and fetal loss (13 weeks of pregnancy or more) were examined microbiologically. In the series of midtrimester fetal loss, bacteria were isolated in 14 per cent and genital Mycoplasma in 37 per cent of the placentas. In induced abortion, 18 per cent of the placentas grew bacteria and in only one case out of 27 was a Mycoplasma strain isolated. Fetal organs yielded cultures positive for bacteria in 16 per cent and for genital Mycoplasma in 23 per cent of the spontaneous fetal loss material, whereas pyogenic cocci were isolated from fetal organs in 18 per cent and Mycoplasma in no case of induced abortion. In the positive cases, the fetal lungs were most often infected (in 34 out of 37 positive cases of spontaneous and 4 out of 5 positive cases of induced abortion). On the other hand, microorganisms could be isolated from the brain in only 5 out of 28 cases of spontaneous and none of induced abortion. In the series of fetal loss, positive microbiologic findings from the fetal organs seemed correlated to prolonged bleeding before abortion or labor and to premature rupture of membranes.

Abortion, Induced↗
PubMedDate not supplied

Infections with Chryseomonas luteola (CDC group Ve-1) and Flavimonas oryzihabitans (CDC group Ve-2) in neurosurgical patients.

During a 20-month period at Temple University Hospital, three cases of infection with bacteria of the Ve group--Chryseomonas luteola (CDC group Ve-1) and Flavimonas oryzihabitans (CDC group Ve-2)--were seen in neurosurgical patients. Two of the patients, including one with what is thought to be the first reported case of meningitis due to C. luteola, had complications caused by prosthetic material. A review of the literature revealed 14 well-described cases of infection with C. luteola or F. oryzihabitans, the majority of which were cases of bacteremia or peritonitis in patients undergoing dialysis. The presence of foreign material and the use of corticosteroids may predispose to and influence the course of disease. As prosthetic material becomes even more widely used, the incidence of infection with bacteria of the Ve group will likely increase and the manifestations of the infections will probably become more varied.

Adult↗
PubMedDate not supplied

Subphrenic abscess simulating metastatic carcinoma.

Subphrenic abscess is reported in two patients, one previously operated on for pancreatic carcinoma and the other for clear cell carcinoma of the left kidney. The subphrenic abscess presented with cachexia and low grade fever six months and one year after surgery. Metastatic carcinoma was erroneously diagnosed in both patients. Despite massive antibiotic treatment, both patients succumbed to sepsis. Because of the inherent diagnostic challenge, delineation of a subphrenic abscess in cancer patients without clear-cut evidence of a metastatic spread is crucial.

Adenocarcinoma↗