[A practical enzymatic cholesterol determination].
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Cholesterol↗
Biomedical subjects
F Stähler
Publications and source records attributed to F Stähler.
[A fully enzymatic triglyceride determination in a continuous flow 12-channel analyzer (author's transl)].
A fully enzymatic triglyceride determination utilizing enzymatic hydrolysis with a lipase-esterase mixture and subsequent enzymatic glycerol determination, has been adapted for use in a continuous flow 12-channel-analyzer. The method is linear up to 7.9 mmol/l (700 mg/100 ml). The analytical precision in the concentration range of 1.5 to 5.4 mmol/l (133 to 478 mg/100 ml) is characterized by relative standard deviations of 0.8 to 4.8%. In the lower measuring range at concentrations around 0.7 mmol/l (62 mg/100 ml) a mean relative standard deviation of 7.2% is found for 1140 measurements under routine conditions. For triglyceride concentrations of 0.9 to 7.7 mmol/80 to 680 mg/100 ml) a mean relative coefficient of verspill Q = 2.0 is determined. Bilirubin caused no observable interference in the determination. In comparison with the manual method by Eggstein & Kreutz (1966) Klin. Wochenschr. 44, 262-267) the results from the fully enzymatic method on the 12-channel-analyzer were lower by approximately 16%, corrected by an additive factor of 0.029 mmol/l (2.59 mg/100 ml). The accuracy controls with controls sera showed a difference of 10%.
Autoanalysis↗
[Enzymatic determination of total cholesterol in serum: accuracy and comparison with other methods (author's transl)].
Cholesterol can be specifically measured without difficulty and without complex reagents by means of a newly developed enzymatic colour test. Intensive technical evaluation confirmed its accuracy. Manual use gave a day-to-day coefficient of variation of 2-3 per cent; the sensitivity at a cholesterol concentration of 200 mg/dl was E equals 0.153 (gamma equals 405 nm), with a linearity up to 1000 mg/dl. Recovery of added pure cholesterol solution was 100 plus or minus 2 percent. A quantitative study of 53 representative drugs, anti-coagulants and metabolites was performed both in test tube and on patients. Accuracy of the result was unaffected by any of the substances (alpha equals 0.05). Comparison with the multi-step extraction method used at present as a reference (Abell-Kendall) gave a regression equation of y equals 0.99 times plus 1.1 (x axis: extraction method; y axis: enzymatic colour test). The direct chemical method after Liebermann and Burchard gave, in part, markedly differing results because of considerable systematic and accidental errors.
Anticoagulants↗