Epithelial displacement.
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Biopsy, Needle↗
Biomedical subjects
F Symmans
Publications and source records attributed to F Symmans.
Fine-needle aspiration of pancreatic extramedullary plasmacytoma: possible confusion with islet cell tumor.
We report two cases of extramedullary plasmacytoma (EMP) of the pancreas, diagnosed by fine-needle aspiration (FNA). Plasmacytoma of the pancreas is a rare neoplasm with only 12 cases recorded in the literature. Because of its scarcity and the cytomorphologic similarity between plasma cells and endocrine cells, EMP of the pancreas may be confused with neuroendocrine (islet cell) tumors of the pancreas. Immunohistochemical staining for light chain and/or neuroendocrine markers will prevent diagnostic error when interpreting plasmacytoid neoplasms of any site susceptible to endocrine tumors, including the pancreas.
Adenoma, Islet Cell↗
Detection of the apoptosis-suppressing oncoprotein bc1-2 in hormone-refractory human prostate cancers.
The oncoprotein encoded by bc1-2 is unique because of its intracellular location (a mitochondrial membrane protein) and apparent mode of action (suppression of apoptosis). To date, this oncogene has been associated only with the development of certain forms of human B-cell lymphoma. In this report, we describe our experience with a monoclonal antibody made against a synthetic peptide for bc1-2 that can recognize the bc1-2 protein and identify cells in human prostate glands expressing this proto-oncogene with in situ immunohistochemical procedures. These procedures were utilized to survey a series of 62 human tissues to evaluate whether bc1-2 might have a role in the developing prostate gland or in prostate oncogenesis. While all primordial epithelial cells in a fetal prostate gland immunostain for bc1-2, normal and hypertrophic prostate glands of the adult show bc1-2 expression restricted to the basal cells. All epithelial cells in areas of prostatic intraepithelial neoplasia were stained by this antibody, as were most (62%) localized invasive prostatic carcinomas. In contrast, all primary prostatic carcinomas and metastases obtained from metastatic prostate cancer patients after hormone treatment (hormone-refractory tumors) stained positive for bc1-2. This study demonstrates that the oncoprotein encoded by bc1-2 can be detected at sequential stages in the natural history of human prostate cancer. Since the bc1-2 oncoprotein is known to suppress the cellular response to apoptotic stimuli, it will be important to determine whether bc1-2 expression is a factor in the development of prostate cancers and in the survival of hormone-refractory prostate cancer cells.
Antibodies, Monoclonal↗
Immunohistochemistry and proliferative activity in Lhermitte-Duclos disease.
We have evaluated a recurrence of Lhermitte-Duclos disease by immunohistochemistry for Purkinje cell markers and proliferative activity (proliferating cell nuclear antigen), by electron microscopy and for DNA ploidy (image analysis). While most of the abnormal neurons in the lesion appear to be derived from granule cells, several Purkinje cell specific polyclonal and monoclonal antibodies, including L7, PEP 19 and calbindin, labeled a minor subpopulation. Staining with monoclonal antibodies to proliferating cell nuclear antigen and measuring cell DNA index and ploidy with a cell image analyzer revealed no proliferative activity. Electron microscopy findings were similar to those previously reported. In spite of its recurrence, our findings support the notion that Lhermitte-Duclos disease is malformative, not neoplastic, and that the characteristic neurons are derived predominantly but not exclusively from a non-Purkinje cell source, probably the granule cell.
Brain↗
Correlation of cervical cytology and human papillomavirus DNA detection in postmenopausal women.
This study correlated the cytological features of cervical swabs from postmenopausal women with the histological findings and the detection of human papillomavirus (HPV) using filter hybridization analysis. Of 17 postmenopausal women seen at colposcopy for an abnormal Papanicolaou (Pap) smear, most often squamous atypia, HPV DNA was detected in one (6%) cervical swab. Biopsy-proven cervical squamous intraepithelial lesions (SILs) were noted in two of the 17 (12%). The HPV DNA detection rate was equivalent to that found in 47 postmenopausal women who had hysterectomies for noncervical disease. The rates of HPV detection and biopsy-proven SIL in premenopausal women seen at colposcopy was 55% and 66%, respectively. The HPV DNA detection rate for postmenopausal women with biopsy-proven SILs as determined by in situ hybridization was 19/26 (73%), which is equivalent to the rate in premenopausal women. It is concluded that squamous atypia in postmenopausal women is rarely associated with either biopsy-proven SIL or HPV DNA detection and thus, in many cases, may represent atrophic changes.
Aged↗
Human papillomavirus detection in adenocarcinoma of the anus.
Human papillomavirus (HPV) is associated with carcinoma of the cervix but not with carcinoma of the endometrium. HPV 16 is the type most commonly detected in squamous cell carcinomas from this site, whereas HPV 18 predominates in adenocarcinomas. We analyzed eight anal carcinomas for HPV DNA using the polymerase chain reaction and type-specific (open reading frame E6) primers for HPV 16, 18, 31, and 35. HPV DNA sequences were amplified in two of six anal adenocarcinomas and, in each case, the type was HPV 18. Sequences homologous to HPV 16 were amplified in each of two anal squamous cell carcinomas; one also contained HPV 18. No amplification was detected in any of seven adenocarcinomas of the rectum or colon or in three adenomatous polyps of the colon. It is concluded that HPV is associated with anal adenocarcinomas but not colorectal adenocarcinomas. The reason(s) why HPV is associated with adenocarcinoma of the anus and cervix but not with the rectum and endometrium, despite the close proximity, requires further study.
Adenocarcinoma↗