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F T Pishotta

Publications and source records attributed to F T Pishotta.

12 recordsLinked to original sources
PubMed1984

Intermediate cell markers for malignancy. Consistency of expression.

Studies of samples from a larger patient population confirmed the consistency of expression of the previously reported markers for malignancy in normal-appearing intermediate cervical cells in samples from patients with abnormal cytology (moderate dysplasia and severe dysplasia/carcinoma in situ). Based on samples of only 30 cells per case, a false-negative rate of 10% to 30% was estimated. The expression of the marker features thus provides a clear indication of uterine abnormal cytology; the lack of expression, however, does not entirely rule out the possibility of uterine abnormalities. The use of larger sample sizes and better staining protocols could further enhance the usefulness of marker feature studies in the prescreening for cancer.

Carcinoma in Situ↗
PubMed1983

High-resolution color video cytophotometry.

Comparison was made between cytophotometric measurements obtained using two data acquisition systems, one a microphotometer and the other a rapid video camera system, to ascertain whether the degradation of data with the faster video acquisition system still results in recorded images of sufficient quality to permit computer discrimination between cells of very similar appearance. Normal-appearing intermediate cells from cases with normal cytology and those from patients with dysplasia or malignant disease, as well as the subvisual markers within these cells that have rendered them capable of cytophotometric discrimination, were used for the study. Comparison of the data recorded by the two systems indicates that the diagnostic information is preserved in the change-over to a full-field, video-rate scanning system, with differences in the data caused primarily by differences in the spectral response of the two systems. This was reflected in the substantial differences observed in the color-related features and the lesser differences seen in the textural features, while the morphometric features (outline and shape) were virtually unaffected. The differences were primarily expressed on a cell-to-cell basis; in sets of about 300 cells, which would be used in patient-to-patient comparisons, the feature values showed remarkable consistency between the two systems.

Color↗
PubMed1983

Marker features for malignancy in ectocervical cells. Statistical evaluation.

Marker features for malignancy have recently been observed in ectocervical cells, even in cells that are visually normal in appearance. This study assessed the statistical significance of these marker features using a mixed-model nested-design analysis of variance (ANOVA). Features in blue intermediate cells from patients with normal cytology, moderate dysplasia, and severe dysplasia/carcinoma in situ, nonkeratinizing cells from patients with moderate dysplasia, severe dysplasia/carcinoma in situ, and invasive cancer, and dysplastic cells from areas of metaplasia from patients with moderate dysplasia, severe dysplasia/carcinoma in situ, and invasive cancer were tested. ANOVA clearly demonstrated that the marker features differentiate between cells of the same cell type originating from patients in different diagnostic categories. In every instance, the differences owing to the diagnostic category were statistically significantly greater than those caused by patient-to-patient variability. Although the discriminating marker features in the intermediate cells were almost exclusively spectral features reflecting staining differences, morphometric features were also marker features in the dysplastic cells.

Analysis of Variance↗
PubMed1983

A quality-control procedure on cervical lesions for the comparison of cytology and histology.

A new method for the quality control of cytologic and histologic diagnoses of cervical lesions is based on the automated high-resolution scanning, image processing and computer analysis of cytometric data by the TICAS system. It determines and then compares optical-density-based ploidy patterns of cells in cytologic smears and the corresponding histologic sections, with the results available both as computer-graphic displays and printouts. Examples of the former appear for an "agreement case," in which the cytologic and histologic patterns corresponded, and a noncorrespondence (nonrepresentative) case, in which the tissue sample had been nonrepresentative of the lesion sampled by cytology. Computergraphic examples concern one case of condyloma and one of tissue repair, in both of which both the cytologic and histologic diagnoses had been overcalled. A further example shows the method's use in monitoring response to therapy. The DNA ploidy patterns on which this method is based can give diagnostic and prognostic clues when morphology alone may be equivocal or insufficient. The utility of ploidy pattern determinations of material from other body sites is also well established. With the use of microprocessors, the system described could be made inexpensive and operationally simple for the routine quality control of many cytopathologic studies as well as for the clinical follow-up of patients.

Adult↗
PubMed1983

The rejection of noncellular artifacts in Papanicolaou-stained slide specimens by an automated high-resolution system. Identification of important cytometric features.

The important cytodiagnostic features that permit discrimination of typical cell types by high-resolution image analysis and pattern recognition techniques have been previously studied in detail. An automated system for the diagnosis of Papanicolaou-stained specimens must also deal, however, with the "real world" of extraneous noncellular artifacts and debris found on every slide. Features that are ideal for the separation of typical normal and abnormal cells may not be adequate by themselves to reject these objects. A new set of discriminatory features must be found. In order to identify those features, a large set of images acquired using the TICAS high-resolution television rapid-scanning system was analyzed and studied. These images, from a variety of slide types, included normal cells, abnormal cells and noncellular artifacts identified by low-resolution preprocessing logic as suspicious enough to warrant high-resolution study. The results indicate that the more important features for such discrimination are not those traditionally important in distinguishing abnormal from normal cells but include color relations, shape measures, boundary properties and texture features.

Cell Separation↗
PubMed1983

A system for reporting gynecologic procedures. A linguistic-logical approach.

A system was developed for reporting gynecologic procedures at Chicago Lying-In Hospital. The system is designed to report procedures and treatments performed during both inpatient stays and outpatient visits. Key features of the system are (1) the automatic encoding of all gynecologic procedures in both the ICD (International Classification of Diseases) and CPT (Current Procedural Terminology) coding systems, (2) the use of an independent program to maintain and update a multiply referenced network of operative procedures and (3) the use of a cross-indexing system that allows each procedure to be accessed in several ways and according to several patterns of keys (billing code number, CPT category, common abbreviation and/or linguistic feature).

Computers↗
PubMed1980

An OCT-reactivity classification to predict fetal outcome.

Based on analyses of the oxytocin challenge test (OCT) in 293 cases, an OCT-reactivity classification is proposed to improve the predictive value of OCT for fetal outcome. All of the 6 perinatal deaths occurred in the 2 groups associated with nonreactive pattern. The positive nonreactive test had 90% accuracy of predicting a sick infant, and the negative reactive test assured good fetal outcome. A nonreactive pattern seems to reflect a compromised fetus, as a high incidence of this pattern was found in patients with pre-eclampsia-hypertension, intrauterine growth retardation (IUGR), low and falling estriol levels, and suspected fetal problems. A positive OCT seems to reflect the limits of fetoplacental reserve under the stressed condition. A high incidence of positive OCT was associated with IUGR, low estriol levels, and a small placenta.

Adult↗
PubMedDate not supplied

Diagnostic marker displays for intermediate cells from the uterine cervix.

Studies have shown the existence of computer-recognizable subvisual markers in intermediate cells from different cervical conditions that show good potential for discriminating between such "normal" cells from patients with normal, severe dysplasia/carcinoma in situ or invasive cancer cytologies. This paper presents examples of computer graphic displays, based on these markers, that could provide recognizable diagnostic clues to the cytologist, with a discussion of some of the factors involved in the interpretation of such displays.

Cervix Uteri↗
PubMedDate not supplied

Diagnostic marker features in dysplastic cells from the uterine cervix.

A study of dysplastic cells, visually classified as non-keratinizing (DYSN), keratinizing (DYSK) or severely dysplastic from an area of metaplasia (DYSM), demonstrated statistically significant differences between the same type of cell depending on the diagnostic category of the patient in which it originated: cases with moderate dysplasia (MOD), severe dysplasia/carcinoma in situ (CSD) or invasive carcinoma (INV). The results suggest that the cell types should include an indication of their origin, that, e.g., DYSN is an insufficient designation and that such cells should be denoted as DYSN-MOD, DYSN-CSD or DYSN-INV; this has major implications for the collection of cell image data bases for quantitative cytology as well as for the effort to characterize patients' cytologic samples by "cytodiagnostic profiles." The results also indicate that the samples as a whole contain many clues to patient diagnosis; examples are given of diagnostic profiles and the related computergraphic displays for different patient categories.

Carcinoma in Situ↗