Biomedical subjects

F Tóth

Publications and source records attributed to F Tóth.

At least 19 recordsLinked to original sources

PubMed2006

Improving the reproductive efficiency by zoo-technical methods at a dairy farm.

This trial was conducted on a Hungarian dairy farm between July 2001 and December 2004. The objective of this work was to improve the reproductive efficiency with relatively high milk production. At the beginning of this trial blood and fodder samples were taken for checking the metabolic status of the animals in order to determine their health condition. The nutritive value of the daily ration for all groups met with the requirements of the Hungarian National Standard and almost all serum metabolic parameters differed between the milking and pregnant animals. Early pregnancy detection (by ultrasound) and ovulation synchronizing methods were introduced to optimize reproductive performance. The oestrus cycle was also checked by ultrasound and open cows were treated by the appropriate method in order to inseminate them as soon as possible. Efficiency of artificial insemination (AI) followed by a single prostaglandin F2alpha (PGF2alpha) and Ovsynch treatment was similar (30.8% and 29%) and less effective than AI after natural heat detection (37.1%). Provsynch (Pre-synch followed by Ovsynch) was the most effective ovulation synchronization method (conception rate=42.6%; p<0.01). Although milk production increased between 2002 and 2004 by approximately 600 kg per cow, the calving period decreased by 20 days and the number of AIs per pregnancy was also improved (0.8 AI per conception). These findings are really beneficial economically because the decrease in the calving interval returns profit for the dairy farms; one open day costs euro2.5/cow. Economical analysis showed a high profit ratio from the reproductive 'investment' on this farm. Every invested euro yielded approximately euro10.

Animal Feed↗

PubMed2005

Behavior and characteristics of biogenic amines, ornithine and lysine derivatized with the o-phthalaldehyde-ethanethiol-fluorenylmethyl chloroformate reagent.

The stability and characteristics of the ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Diah), spermidine (Spd) and spermine (Spm) derivatives obtained with the o-phthalaldehyde (OPA)-ethanethiol (ET)-fluorenylmethyl chloroformate (FMOC) reagent has been investigated. The stoichiometry of the introduced, two-step derivatization process has been followed by photodiode array (DAD) and fluorescence (FL) detections, simultaneously, while the composition of derivatives was confirmed by on-line HPLC-electrospray ionization (ESI) MS measurements. Depending on the composition of the OPA reagents, in addition to the secondary amino group-containing Spd and Spm, under common aqueous conditions also Orn and Lys do react with FMOC resulting in derivatives of various compositions. Applying the OPA-ET reagent of increasing methanol (Met) content (38-80%, v/v) the formation of the FMOC group containing Orn and Lys derivatives could be considerably decreased. Optimum elution condition (18 min, including equilibration) was developed for the simultaneous quantitation of Orn, Lys, Put, Cad, Diah, Spd and Spm, in the presence of the rest of protein amino acids. The practical utility of the method was demonstrated by the analysis of mouse tissues. Average reproducibility of quantitations, characterized with the relative standard deviation percentages of fluorescence intensities and UV responses, in order of listing, proved to be 2.1% and 2.1%, respectively.

Biogenic Amines↗

PubMed2004

o-Phthaldialdehyde derivatization of histidine: stoichiometry, stability and reaction mechanism.

The irregular behavior of histidine in its reaction with the o-phthaldialdehyde (OPA) reagents has been studied. Histidine provides more than one OPA derivative. Similarly to all those primary amino group-containing compounds that do have in their initial structure the -CH2-NH2 moiety. The ratio of histidine's initially formed and transformed OPA derivatives depends on the temperature: very likely due to the fact that elevated temperature favors the intra-molecular rearrangement of histidine resulting in the formation of the -CH2-NH2 moiety-containing tautomer(s). The higher the temperature the higher the amount of the transformed species. The composition of the initially and transformed OPA derivatives of histidine were identified on the basis of their on-line HPLC-electrospray ionization (ESI) MS spectra and computations. The initially formed species has been identified as the classical isoindole, while the transformed one contains an additional OPA molecule.

Amino Acids↗

PubMed2004

Behavior and characteristics of the o-phthaldialdehyde derivatives of n-C6-C8 amines and phenylethylamines with four additive SH-containing reagents.

The stability and characteristics of the C6-C8 n-aliphatic and phenylethylamines have been investigated as their o-phthaldialdehyde (OPA)/3-mercaptopropionic acid, OPA/N-acetyl-L-cysteine, OPA/2-mercaptoethanol and OPA/ethanethiol derivatives. Stoichiometric studies have been followed by photodiode array and fluorescence detection, simultaneously, while the composition of derivatives was confirmed by on line HPLC-electrospray ionization (ESI)-MS measurements. All four amines having in their original structure the NH2-CH2- moiety in accordance with the C1-C4 aliphatic, mono and diamines and amino acids of the same structure--furnished more than one OPA derivative: their initially formed isoindoles transform to further ones. Depending on the composition of the OPA reagents and on the pH of derivatizations different type of transformed species have been identified, in various proportions. Applying the OPA/SH additive reagent in the molar ratio of 1/3, favors the formation of one additional OPA molecule-containing isoindole, while using the OPA/SH additive (1/50) reagent resulted in the formation of one additional SH additive-containing species, identified and measured at the first time by HPLC. Transformation rate and stability of derivatives proved to be associated with the composition of the OPA reagent, with the type of the SH additive, with the pH of derivatizations, and, in selected cases also with the chain length of the amine. Results of stoichiometric and mechanism studies have been utilized to define optimum analytical conditions.

Amines↗

PubMed2003

The role of HLA-DRB1*04 alleles and their association with HLA-DQB genes in genetic susceptibility to rheumatoid arthritis in Hungarian patients.

We investigated the HLA-DRB, and DQB polymorphism and haplotypes in RA subjects of Hungarian origin by PCR typing using sequence-specific primers. Molecular subtyping of HLA-DRB1*04 alleles in RA patients showed strongest association with highest relative risk with DRB1*0404. A significantly decreased frequency of DRB1*0403 was observed in patients compared to controls. A significant number of patients carried DR4 haplotypes on DQB1*0302 (54%) relative to DQB1*0301 which was present on 36% of the haplotypes. When compared to controls, the frequency was higher in the latter allele only. Few unique DRB-DQB haplotypes were observed in Hungarian RA patients. In spite of the fact, that the Hungarian population has been isolated linguistically over centuries, a considerable racial admixture has occurred following immigration and invasions, thus the present study confirms in Hungarian patients with RA, previous findings for RA and HLA in European countries.

Alleles↗

PubMed2002

Advances in the evaluation of the stability and characteristics of the amino acid and amine derivatives obtained with the o-phthaldialdehyde/3-mercaptopropionic acid and o-phthaldialdehyde/N-acetyl-L-cysteine reagents. High-performance liquid chromatography-mass spectrometry study.

The composition of the amino acid and amine derivatives obtained with the o-phthaldialdehyde (OPA)/3-mercaptopropionic acid (MPA) and with the OPA/N-acetyl-L-cysteine (NAC) reagents was investigated by on-line HPLC-electrospray ionization MS. The initially formed derivatives proved to be, as expected, the corresponding isoindoles while their transformed species contained one additional OPA molecule. Based on the MS spectra of all transformed OPA derivatives a reaction pathway is suggested. This reaction mechanism was supported both by the molecular ions of the endproducts and by the presence of several selective fragment ions that served as an explanation to the structure of the believed to be less stable OPA derivatives. It has been shown that more than one OPA derivative forms in all those cases when the compound to be derivatized does contain the NH2-CH2-R moiety. Thus, amino acids like e.g. glycine, histidine, beta-alanine, gamma-aminobutyric acid, epsilon-aminocaproic acid, ornithine, and also several aliphatic mono- and diamines provide more than one OPA derivative. Analytical consequences of this experience were utilized by altering the reagent's composition. Reagents containing mole ratios of [OPA]/[MPA] or [OPA]/[NAC]=1/50 resulted in two benefits, simultaneously: (i) in a decrease of the transformation rate of the initially formed derivative, and, (ii) in an increase of the overall stability of the total of derivatives.

3-Mercaptopropionic Acid↗

PubMed2001

[Endometrial carcinoma in the young].

The authors aim is to draw the readers attention to the possible occurrence of endometrial carcinoma in the young, by presenting the case of a 23 year-old woman. They highlight the risk factors (obesity, anovulatory bleeding abnormality, hypertension, positive familial history), that may have lead to the development of the endometrial carcinoma of their patient. It is assumed, that bleeding abnormality of a young woman could be the first sign of the carcinoma of the uterine corpus. They consider it obligatory in similar cases to: 1. Perform a throughout investigation of the endocrinological status 2. Measure the thickness of the endometrium with ultrasound 3. Histological examination 4. Careful follow up.

Adult↗

PubMed2001

Distortion-product otoacoustic emission (DPOAE) following pure tone and wide-band noise exposures.

The aim of our investigations was to determine how the intensity of distortion-product otoacoustic emission (DPOAE) changes following different sound and noise exposures. We performed examinations on 20 healthy people with normal hearing. DPOAEs were recorded scanning the 0.5-6 kHz frequency interval before and after the exposures. We exposed the subjects to 0.5, 1, 2, 4 and 6 kHz pure tones and wide-band noise (intensity: 80 dB HL, duration: 3 minutes). We conclude that the amplitudes of DPOAEs changed immediately after exposures at most frequencies. DPOAE intensities decreased at some frequencies in the middle frequency range (1-2 kHz), and increased at low and particularly at high frequencies.

Adult↗

PubMed2001

Brainstem auditory-evoked potential examinations in diabetic patients.

Brainstem auditory-evoked potential (BAEP) examinations were performed in 15 patients with long-standing type-1 diabetes mellitus (DM). Cardiovascular reflex tests were applied for assessment of autonomic neuropathy. The aim of our investigation was to compare the BAEP results of this patient group with controls and to look for a possible correlation between the alteration of the auditory brainstem function and the cardiovascular autonomic neuropathy. Analysis of the latencies (waves I, II, III and V) and the inter-peak latencies (waves I-III and I-V) of BAEPs revealed a significant difference between diabetics and healthy controls. The amplitudes of waves I, III and V were definitely lower in comparison with those of healthy controls. A positive correlation was observed between the overall autonomic score and the latencies (waves III and V) and inter-peak latencies (waves I-III and I-V). These data support the hypothesis that long-standing DM and diabetic neuropathy might be related as a cause of certain dysfunctions of the central auditory pathways.

Adult↗

PubMed1998

The prognostic value of facial nerve conduction velocity in patients with Bell's palsy.

The facial nerve conduction velocity was measured in 30 healthy subjects (60 sides) and in 51 patients with a unilateral Bell's palsy. The normal value was 47.8 +/- 5.1 m/s. Incomplete recovery was common in Bell's palsy when the velocity was below 30 m/s. Mild synkinesis was observed in only one patient when the nerve conduction velocity was above 30 m/s. When the degree of degeneration revealed by electroneuroneography did not exceed 60%, the conduction velocity was in the normal range. For degrees of degeneration in excess of this, the conduction velocity decreased in parallel with the increase in the degree of degeneration.

Action Potentials↗

PubMed1998

Cochlear implantation in osteogenesis imperfecta.

A case is reported in which a Nucleus 22 channel intracochlear implant was applied to a Hungarian woman (age 50 yr) with profound deafness associated with osteogenesis imperfecta. Successful intracochlear insertion of the 22 electrodes resulted in a 70 dB hearing improvement at frequencies 250-2000 HZ. Nevertheless, a characteristic facial twitching appeared upon activation of electrodes 9-13. Inactivation of these electrodes abolished the non-acoustic nerve excitation with preservation of acoustic performance. Osteogenesis imperfecta may involve a state of risk for non-acoustic nerve activation in cochlear implant patients possibly resulting from a reduced impedance to current spread by abnormal bone tissue. This, however can be overcome by simple programming manoeuvres.

Cochlear Implantation↗

PubMed1996

Survival of model bacterial strains and helminth eggs in the course of mesophilic anaerobic digestion of pig slurry.

The effect of methanogenesis on the survival of model bacterial strains (Escherichia coli EC 5, Staphylococcus aureus SA 11, Enterococcus faecium CCM 4231) and non-embryonated helminth eggs (Ascaris suum) was investigated in pig slurry. Two pilot-plant experiments were carried out in two anaerobic digesters (800 and 1,000 litre) in a mesophilic thermal range (35-37 degrees C). The mean hydraulic retention time of the digesters was 20 days. The methanogenesis process was monitored by determining the following chemical parameters: pH, N-NH3, total dry matter (kg/day), organic matter (kg/day) production of methane by supplied and degraded organic matter (m3/kg). The results obtained allow us to state that the anaerobic stabilization of pig slurry in the mesophilic temperature range resulted in total devitalization of model bacterial strains E. coli EC 5 and Ent. faecium CCM 4231. St. aureus SA 11 cells, exposed to the above mentioned conditions, were also reduced in their number from 10.04 to 3.27 and from 8.69 to 2.77 log cfu/ml. It is assumed that the longer retention time of excrements in the digester could also result in total devitalization of St. aureus SA 11 cells. From the microbiological point of view, the above mentioned facts indicate a sufficient hygienization effect of the anaerobic fermentation on the contaminated pig excrements. The survival of A. suum eggs was little affected by the 20-day anaerobic mesophilic digestion of pig slurry. Only 17 or 18% (F1, F2) of the non-embryonated A. suum eggs were damaged after the 20-day exposure.

Animals↗

PubMed1996

Molecular genetic analysis of HLA-DRB, -DQA, and -DQB polymorphisms in Hungarians and distribution of the DRB1*03 allele in adults and newborns.

We describe here the genetic variability of HLA-DRB1, -DQA, and -DQB in 140 healthy individuals from Hungary, including 95 randomly selected adults and 45 newborns. Allele and haplotype frequencies as well as linkage disequilibria were calculated. It was found that HLA-DRB1*11, -DQA1*0501, and -DQB1*0301 predominate in Hungarians. This information may be helpful in the future for HLA and disease association studies. Simultaneously, we observed that the frequency of the DRB1*03 allele differs between adults and newborns. Since it is well known that bearers of HLA-DR3 (and/or B8) antigens may display significant changes in immune parameters, the lower frequency in adults indicates that children with the DR3 antigen are predisposed to immune diseases in adulthood.

Adolescent↗

PubMed1994

[Eosinophilic leukemia: a rare form of Philadelphia chromosome negative chronic myeloid leukemia?].

Eosinophil leukaemia is a rare and poorly defined entity characterized by neoplastic proliferation of eosinophil cell line. This form of the hypereosinophilic state is considered to be a variant form of CML, although as a diseases entity is not generally accepted. A history of a patients is reported, whose clinical course is thought to fulfill the requirements of eosinophil leukaemia. On the basis of the initial results (pathological lymphogram, eosinophilia, Ph-negativity) lymphogranulomatosis was suspected and explorative laparotomy was performed. However, only marked eosinophilic infiltration of the spleen was detected. After splenectomy his disease was stable without treatment for six months when his leukocytosis and eosinophilia increased. Despite the administration of hydroxyurea the leukocyte count exceeded 100 x 10(9)/l (eosinophil cells 70%), and the bone marrow revealed massive (80%) eosinophilic infiltration. Neither Ph-chromosome, nor cabl and bcr gen rearrangement were demonstrated, but the expression and amplification of c-myc oncogene indicated disease progression. Interferon therapy produced long-term clinical and haematological improvement, but blastic transformation was developed in the second year of his disease. Autopsy showed multiple organ involvement characteristic of CML, but no marked eosinophilic infiltration was found. The feature of this case suggest that eosinophil leukaemia might represent an uncommon form of Ph-negative CML.

Adult↗

PubMed1993

[The effect of experimental aerobic stabilization of swine slurry on survival of Salmonella typhimurium and Ascaris suum].

Laboratory aerobic mesophilic stabilisation (fermentation) of pig slurry reduced the survival time of S. typhimurium, compared with their prevalence in anaerobic excrements. The decimation time T90 for S. typhimurium, was 32 hours. The effect of aerobic stabilisation on the survival of nonembryoed eggs of the model helminth A. suum was lower than that observed in Salmonella. After 54 hours of aerobic stabilization of slurry only 32% of eggs were devitalized. Stabilized control group did not develop 13% of eggs into their embryonated stage.

Aerobiosis↗

PubMed1991

[Distal osteotomy of the metatarsal bones in the treatment of metatarsalgia].

Authors review briefly the development of the principles of metatarsal osteotomies and the basic concepts that seem to be the most suitable in the choice of the type and execution of the operation. The effectiveness of the surgical treatment is stressed if the following viewpoints are considered: 1. Synchronous correction of the rays II, III, and IV. 2. Simultaneously achieved abbreviation and forming of the arch. 3. Instead of internal fixation transversal lifting bandage with early weight bearing. The finding, according which, beside the telescope osteotomy of Helal, Wolf's metatarsal osteotomy with axial deviation is also a valuable alternative, is supported by the follow-up of their own 2 years material.

Humans↗

PubMed1991

[Management of recurrent patellar dislocation by semitendinous muscle transfer].

After review of the pertinent literature authors report on a new tendon transfer technique not having been found in the available literature. The method has been considered suitable for the treatment of patellar dislocations due to erroneous stretching direction occurring before the end of growth. The semitendinous muscle was separated and transfosed in the tunnel drilled into the patella. By means of this technique the function of the muscle was used against the lateralization tendency of the patella. The paper presents the follow-up of 9 cases.

Adolescent↗

PubMed1990

Detection and determination of factor C--a regulatory protein--in Streptomyces strains by antiserum and monoclonal antibody.

Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34,000 or 70,000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy+ strains were usually good producers of factor C while Amy- were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.

Animals↗