Biomedical subjects

F Taşman

Publications and source records attributed to F Taşman.

12 recordsLinked to original sources

PubMed2001

Expression of beta 1 integrins in human dental pulp in vivo: a comparative immunohistochemical study on healthy and chronic marginal periodontitis samples.

AIM: The objective of this study was to determine the tissue distribution of beta 1 integrin chains in sound human dental pulps and to compare the findings with connective tissue compartments of other organs and to pulp tissue in teeth extracted due to periodontal disease. METHODOLOGY: Freshly frozen pulp tissue samples from teeth extracted for orthodontic reasons were examined and compared to samples from teeth extracted due to chronic (marginal) periodontitis. beta 1 integrin chains were determined using an indirect-immunoperoxidase technique. Seven monoclonal antibodies recognizing alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6 and beta 1 chains of Very Late Activation Antigen (VLA) integrins were used for this purpose. RESULTS: VLA-1, VLA-2, VLA-3 and VLA-5 were expressed by vascular endothelium and vascular smooth muscle in varying intensities in both groups. VLA-6 reactivity was observed in the basal surfaces of arterial, venous and capillary endothelia. Our results indicate that there was no significant difference in the expression of VLA integrins in sound pulp tissue when compared to the samples from chronic (marginal) periodontitis and the connective tissue compartments of other viscera. CONCLUSION: The present findings suggest that human dental pulp tissue is not different from other connective tissue compartments in the body with respect to VLA integrin expression, and chronic marginal periodontitis does not affect pulp tissue to a histopathologically detectable extent.

Antibodies, Monoclonal↗

PubMed2001

Effect of gutta-percha solvents at different temperatures on the calcium, phosphorus and magnesium levels of human root dentin.

The aim of this study in vitro investigation was to evaluate the alterations caused by warmed gutta-percha solvents on the calcium, phosphorus and magnesium levels of root dentin. Extracted human anterior teeth, whose crowns and apical root thirds had been removed were used as root dentin specimens. The roots were sectioned longitudinally into two segments, cleaned and dried. Segments were divided into 12 groups (n=12). In 6 groups, the specimens received treatment with the following solvents at room temperature (22 degrees C): Chloroform, xylene, eucalyptol, orange oil, halothane and saline (control). Within each group, the specimens were further subgrouped into two to be incubated (100% humidity at 37 degrees C) for 5 and 10 min, respectively, following treatment with the solvents. The remaining six groups were treated with the same solvents which had been previously warmed to body temperature (37 degrees C) and received the same experimental procedures. The levels of calcium, phosphorus and magnesium in each specimen were analysed using energy dispersive spectrometric microanalysis. Statistical analysis of the readings showed that neither warming of the solvents nor prolonged incubation (treatment) time was capable of altering the histochemical composition of cut root dentin surfaces.

Body Temperature↗

PubMed2001

Neural cell adhesion molecule and neurothelin expression in human ameloblastoma.

PURPOSE: The objective of the present study was to determine whether neural cell adhesion molecules (NCAM; CD56) and neurothelin (CD147) are expressed by adontogenic cells in the ameloblastoma. MATERIALS AND METHODS: Frozen sections from ameloblastoma specimens (n = 7) were stained with monoclonal antibody-recognizing CD56 and CD147 molecules using indirect immunoperoxidase and indirect immunofluorescent techniques. RESULTS: CD56 and CD147 molecules were strongly expressed by the peripheral columnar cells of the tumor nests. Neurothelin reactivity was also present in the stellate reticulum of the nests and in some stromal components. CONCLUSIONS: The presence of these antigens in ameloblastoma supports the classic view about the neural crest origin of cells giving use to this tumor.

Ameloblastoma↗

PubMed2000

Endothelial cell and stromal antigens in human periapical granulation tissue.

Periapical granulation tissue consists of vasculature of varying sizes and types, infiltrating cells, and other stromal elements. We examined the differential expression of endothelial and stroma antigens in this tissue to determine their tissue distribution in order to obtain hints on their functions. Some of the antigens examined were present only in the endothelial lining of vasculature, including high endothelial venules (e.g. CD31 and CD105), whereas others were more widely expressed by both vascular and stromal elements (e.g. CD29, CD63, CD44, and CD151). Immunohistochemical analysis using monoclonal antibodies specific to certain tissue compartments revealed the tissue architecture more precisely and the expression of certain antigens in the tissue suggested special roles for these antigens. Tissue distribution of CD63, CD143, CD147, and CD151 in periapical granulation tissue is first reported in the present study.

Antibodies, Monoclonal↗

PubMed2000

CD45/isotypes expression in the immune cells of human periapical lesions.

Expression of some leukocyte antigens (including CD45) and its isoforms (CD2, CD4, CD5, CD6, CD7, and CD8) was examined in the human periapical granulation tissue samples in the present study. The majority of the infiltrating cells expressed heavy molecular-weight isoforms of the CD45 antigen. Expression of CD2, CD5, CD6, and CD7 antigens was also detected, implying significant roles for these antigens in the immune reaction taking place in periapical lesions. This suggests that the immune response taking place at the periapical region is predominantly cellular and the humoral responses to antigenic challenge are conducted mainly by regional lymph nodes.

Antibody Formation↗

PubMed2000

Effect of a fifth-generation bonding agent on vascular responses in rats.

In the last years, several single-bottle dental adhesives have been developed. They are supposed to chemically adhere to dentin, and a liner to protect pulp is not thought to be necessary. In the case of direct pulp capping, hemorrhage control is an important step in success, but little is known about the effect of these new bonding agents on pulpal bleeding. The aim of this study was to evaluate the effect of a new one-step single-bottle adhesive on the smooth muscle contraction of rat uterine muscle and carotid arteries. Efficacy of the bonding agent was compared with that of epinephrine (control), and the contraction forces induced were recorded using a force displacement transducer. Statistical analysis of data revealed that the bonding material produced epinephrine-like concentration-dependent contractions of the smooth muscle, suggesting that one-step bonding agents may act as candidates for hemorrhage control without the use of an additional agent in direct pulp capping procedures.

Analysis of Variance↗

PubMed2000

A comparison of the surface tension of calcium hydroxide mixed with different vehicles.

The aim of this study was to evaluate and compare the surface tension of different liquids that are used as potential agents to mix with calcium hydroxide in root canal treatment. We also tried to evaluate final surface tension values of the calcium hydroxide mixed with glycerin, Ringer's solution, anesthetic solution, and saline. Surface tension values were measured using a "Du Noüy" tensiometer. The results showed statistically significant differences between vehicles alone and the calcium hydroxide combinations and also indicated that anesthetic solution (p = 0.018, p < or = 0.02) is the most favorable vehicle with the lowest surface tension values.

Analysis of Variance↗

PubMed2000

Surface tension of root canal irrigants.

The aim of this study was to evaluate the surface tension values of established and potential endodontic irrigants to which a surface active agent had not been added. Additionally, Cetredixine, a surfactant-containing 0.2% chlorhexidine gluconate solution, was included in the measurements. Surface tension measurements were performed using the ring method on a DuNouy tensiometer at a standard room temperature. Ringer's solution, saline solution, and distilled water had the highest surface tension values, whereas those of NaOCl (2.5% and 5%) and 17% EDTA were relatively low. Two anesthetic solutions, Ultracaine and Citanest, demonstrated values similar to NaOCl and EDTA, although a statistically significant difference was found between all solutions tested. Cetredixin displayed the lowest surface tension. A low surface tension agent should penetrate tubules better.

Analysis of Variance↗

PubMed2000

Effect of different single-bottle dentin adhesives on vascular responses in rat carotid artery.

PURPOSE: To investigate the effect of one-bottle dentin bonding systems on pulpal hemorrhage control in direct pulp exposures through contraction of pulp blood vessels using the rat carotid artery model. MATERIALS AND METHODS: Four dentin bonding agents (Prime & Bond 2.1, Prime & Bond NT, Syntac Single Component and Single Bond) were used. The efficacy of bonding agents were compared to that of epinephrine (control) in the viewpoint of the concentration applied and the contraction forces induced on the smooth muscle were recorded using a force displacement transducer. RESULTS: Three of the test materials produced epinephrine-like contractions on the rat carotid artery (smooth muscle) while no reading could be achieved with Single Bond. A ranking between the bonding agents for contraction forces was obtained as follows: Prime & Bond NT > Prime & Bond 2.1 > Syntac Single Component (P < 0.05).

Analysis of Variance↗

PubMed1999

Endothelial cell adhesion molecules in human dental pulp: a comparative immunohistochemical study on chronic periodontitis.

Migration of leukocytes to inflammation sites through vascular endothelium is controlled by the interactions of adhesion molecules expressed on both endothelial cells and leukocytes, most of which are already covered by cluster of differentiation (CD) codes. We examined the expression of a variety of endothelial cell adhesion molecules in human dental pulp vasculature to obtain further evidence on the tissue distribution and function of these molecules by using an indirect immunoperoxidase technique. We obtained the pulp tissue samples from teeth extracted due to orthodontic reasons as controls and compared with those extracted due to chronic periodontitis. In all samples, both CD31 and CD146 were expressed by arterial, venous, and capillary endothelia. There was no significant difference between the staining intensity of normal and inflamed pulp tissues. CD102 expression on the endothelium was significantly stronger in chronic periodontitis pulp samples. CD106, CD62-E, CD62-P, CD105, and CD54 were variably expressed in control and chronic periodontitis groups. Our results indicate that CD102 represents the major endothelial cell adhesion molecule probably involved in the inflammatory reactions in chronic periodontitis.

Antigens, Differentiation↗

PubMed1998

Combined endodontic-orthodontic and prosthodontic treatment of fractured teeth. Case report.

A case history of a 10 year old patient who accidentally injured his maxillary left central incisor is presented. The fracture of the crown extended about 4 mm below the crest of the alveolar bone. After pulpectomy and root canal therapy, a screw was cemented into the canal of the incisor. By means of a removable orthodontic appliance, the fractured tooth was extruded and the fracture line was brought above the level of the alveolar bone. The tooth was then restored with a veneer crown over a post core. A tooth that would have been extracted routinely was thus saved and restored through the use of a collaborative approach.

Accidental Falls↗

PubMed1997

Solubilizing efficiency of different gutta-percha solvents: a comparative study.

A study was conducted to comparatively evaluate the efficiency of different solvents for dissolving gutta-percha. Halothane, chloroform, xylene, acetone, isopropyl alcohol, turpentine, oil of mela-leuca and eucalyptol were used as solvents for dissolving standardized gutta-percha discs. Halothane, chloroform and xylene were markedly superior solvents of gutta-percha in comparison with the others. There was no significant difference among the three (p > 0.05). Eucalyptol, turpentine and oil of melaleuca were relatively less efficient. Acetone and isopropyl alcohol did not dissolve gutta-percha, being similar in this respect to distilled water.

2-Propanol↗