Biomedical subjects

F Tallet

Publications and source records attributed to F Tallet.

28 records · Page 2Linked to original sources

PubMed1983

[Algodystrophy and metabolic abnormalities].

The French Society of Rheumatology national study of reflex dystrophy revealed a serum glucose greater than 1.20 g/l in 9.09 p. cent of cases, a serum uric acid greater than 70 mg/l in 25 p. cent of men and greater than 60 mg/l in 14 p. cent of women. Serum cholesterol was normal in males, but higher than the mean + 2 sigma in 23 p. cent of females. Serum triglycerides were higher than m + 2 sigma in 55 p. cent of men and 56 p. cent of women. From a group of 80 patients, 54 (67.5 p. cent) had at least one of the three metabolic abnormalities and 49 (61.25 p. cent) were hypertriglyceridaemic. This hypertriglyceridaemia is the most frequent abnormality found. When hyperglycaemia or hyperuricaemia are present it is almost always in association with hypertriglyceridaemia. Hypertriglyceridaemia is more common in algoneurodystrophy of the lower limbs (54/78, 69 p. cent) than in algoneurodystrophy of the upper limbs (5/22, 22.7 p. cent). Algoneurodystrophy is more often primary, when it occurs in the lower limbs and post-traumatic when it occurs in the upper limbs. A prospective study comparing several parameters of lipid metabolism in 24 patients with algoneurodystrophy and 15 matched controls showed a significant drop in the HDL Chol/HDL P1 ratio and in the DHL - HDL Chol/TG ratio in patients with algoneurodystrophy. Serum insulin was comparable in the 2 groups. Lecithin cholesterol acyl transferase (LCAT), SGOT, SGPT and GGT were normal. The essential role of hypertriglyceridaemia in the genesis of the characteristic bony lesions of algoneurodystrophy is discussed.

Adolescent↗

PubMed1982

Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation.

We describe an automated procedure for determination of high-density-lipoprotein cholesterol in serum by use of heparin-MgCl2-albumin reagent. 'AutoAnalyzer' II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by filtering on-line across a cellulose acetate membrane. Cholesterol concentration was measured by the enzymic method of Allain. Good correlation was obtained using the automated method compared to the heparin-MgCl2-albumin (r=0.99) or sodium phosphotungstate-MgCl2 procedure (r=0.97). Contamination was minimal with a contamination coefficient lower than 2.5%. The method presented was linear (0 to 2.50 mmol/1) and could be applied to lipemic samples with a coefficient of variation better than 3%. This study showed thus that this new procedure was suitable for routine work with a good rate (60 samples/h) in almost any laboratory.

Autoanalysis↗

PubMed1982

[Serum amylase determination : automated continuous flow method (author's transl)].

A simple automated assay was described for the determination of serum amylase using a chromogenic substrate. The reaction was stopped by filtration across a cellulose nitrate membrane. The analytic parameters of this method were studied. Repeatability, reproducibility and precision were convenient. The calibration curve was linear up to 850 U/I. An excellent correlation was obtained after comparison with the manual method (Phadebas amylase test). The effects of haemolysis and very high ascorbic concentrations of bilirubin, glucose, uric acid and ascorbic acid were negligible on the value measured. The serum turbidity was alone to induce a variation by excess.

Amylases↗

PubMed1982

[Algodystrophy and metabolic anomalies].

The French Society of Rheumatology national study of reflex dystrophy revealed a serum glucose greater than 1.20 g/l in 9.09 p. cent of case, a serum uric acid greater than 70 mg/l in 25 p. cent of men and greater than 60 mg/l in 14 p. cent of women. Serum cholesterol was normal in male cases, but higher than the mean + 2 sigma in 23 p. cent of female cases. Serum triglycerides was higher than m + 2 sigma in 55 p. cent of men and 56 p. cent of women. From a group of 80 patients, 54 (67.5 p. cent) had at least one of the three metabolic abnormalities and 49 (61.25 p. cent) were hypertriglyceridaemic. This hypertriglyceridaemia is the most frequent abnormality found. When hyperglycaemia or hyperuricaemia are present it is almost always in association with hypertriglyceridaemia. Hypertriglyceridaemia is more common in algoneurodystrophy of the lower limbs (54/78, 69 p. cent) than in algoneurodystrophy of the upper limbs (5/22, 22.7 p. cent). Algoneurodystrophy is more often primary, when it occurs in the lower limbs and post-traumatic when it occurs in the upper limbs. A prospective study comparing several parameters of lipid metabolism between 24 patients with algoneurodystrophy and 15 matched controls showed a significant drop in the HDL Chol/HDL P1 ratio and in the DHL--HDL Chol/TG ratio in patients with algoneurodystrophy. Serum insulin was comparable in the 2 groups. Lecithin cholesterol acyl transferase (LCAT), SGOT, SGPT and GGT were normal. The essential role of hypertriglyceridaemia in the genesis of the characteristic bony lesions of algoneurodystrophy is discussed.

Adult↗

PubMed1980

[Critical aspects of actual methods for high-density-lipoprotein cholesterol quantitation].

In this study we compare various methods for h;igh-density-lipoprotein cholesterol quantitation: ultracentrifugation; electrophoresis either on cellulose acetate or on agarose gel; precipitation methods with polyanions-bivalent cations; adsorption procedure using concanavalin A (Con A). quantification by electrophoresis is slow and not precise. Except heparin-Mn++ and dextran (MM 5 X 10(5) and 2 X 10(6)) - CaCl2 procedures, precipitation and Con A methods own a good repeatability (VC 2%) and give a linear response after VLDL or LDL-VLDL addition to tested sera. Regression analysis of the data obtained using these methods in pairs demonstrate a correct accuracy (r > 0.95). The Student t-test shows that the usual values must be evaluated for each method. We suggest to use either phosphotungstate - Mg++ or Con A procedure as method for HDL cholesterol quantitation.

Adsorption↗

PubMed1980

Heparin--magnesium chloride--albumin method for enzymic measurement of cholesterol in high-density lipoprotein.

We describe a new method for measuring high-density-lipoprotein cholesterol in sera, with use of heparin, MgCl2, and albumin to precipitate very-low- and low-density lipoproteins, Lipoprotein electrophoresis and immunochemical methods show that high-density lipoprotein is completely and specifically isolated in the supernate, the cholesterol concentration of which is estimated by the enzymic method of Allain et al. (Clin. Chem. 20, 470, 1974). The precision of the method is excellent (CV = 1%) and the response is linear. Values so obtained correlate well with those obtained with the heparin-Mn2+ (r = 0.98) or the phosphotungstate-Mg2+ (r = 0.98) procedures. The technique is appropriate for routine clinical laboratory use.

Adult↗

PubMed1980

[Serum phospholipids determination by an automatic enzymatic method (author's transl)].

The serum phospholipids determination by an automatic enzymatic method (AEM) is suggested in this study. A comparison was made between the classical chemical method, the manual enzymatic method (MEM) and the AEM one. 100 serum samples were tested in this experiment and the correlation coefficient between these methods was found to be acceptable. The result indicates a good precision and a negligible contamination. The calibration curve is linear to 5 mmol/l. Triglyceride and cholesterol concentrations, glucose and high concentrated uric acid presence do not interfere on the value measured. However, the haemolysis and a large quantity of bilirubin in sera tend to induce a variation in the phospholipids determination.

Autoanalysis↗

PubMed1979

[Estimation of serum lipids: method without extraction adapted to autoanalysis (author's transl)].

An entirely automatic method, without prior extraction, is described for the determination of total serum lipids by a nephelemetric method. The results are compared with those obtained by Canal's method. There is a good correlation between the two technics. With a similar concentration of lipid the respective quantities of cholesterol and triglyceride do not intervene in the flocculation. The respective influence of the bilirubin concentration, hemolysis, and the concentration and nature of the serum proteins is considered. This method should permit simultaneous autoanalysis of the three parameters of classical lipid investigation: total lipids, triglycerides and cholesterol.

Autoanalysis↗

PubMedDate not supplied

Type IV hyperlipoproteinemia in patients with algodystrophy.

The present work studies lipid metabolism in patients with algodystrophy (AD). A correct positive correlation (r = 0.75) between the triglyceride levels and low density lipoprotein (LDL)/very low density lipoprotein (VLDL) ratio and the VLDL increase observed by gel disk electrophoresis confirm that a type IV hyperlipoproteinemia is associated with AD. In contrast, the degree of high density lipoprotein (HDL) saturation in cholesterol (HDL-cholesterol/HDL-phospholipids) and the classical atherogenous index (cholesterol/HDL-cholesterol) were not modified. The decrease of plasma post-heparin lipolytic activities (PHLA) was not significant but further studies should be performed to correlate PHLA with a reduced activity of the adipose tissue lipoprotein lipase.

Cholesterol↗

PubMedDate not supplied

[Enzyme-immuno assay for total estrogens and human placental lactogen. Comparison with radio-immuno-assay in normal pregnancy-monitoring (author's transl)].

The concentrations of estrogens (E) and human placental lactogen (HLP) are estimated in sera by radio immuno-assay (RIA) and enzyme-immuno-assay (EIA). Statistical data indicate mean intra-assay variation coefficients of 7% and 12% for E and HLP tests, respectively. The correlation coefficient (RIA/EIA) are found higher than 0,9% for both hormonal assays. The dilution curves obtained by RIA and EIA are similar. However, Student'test gives a significant difference for E determination. In fact, total E and E 3 only are measured by EIA and RIA, respectively. In most cases biological interferences are negligible except for HLP in presence of higher protein or haemoglobin levels. RIA and EIA are performed to study serum HLP and E levels throughout normal pregnancies. Results allow to use EIA for HLP and E evaluations in pregnancy-monitoring.

Adolescent↗