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F Tron

Publications and source records attributed to F Tron.

At least 109 records · Page 6Linked to original sources
PubMed1988

Intestinal secretory antibody response induced by an oral cholera vaccine in human volunteers.

The ability is reported of a new oral cholera vaccine composed of Vibrio cholerae antigenic fractions to induce a serum and mucosal antibody response after three oral administrations of microgranules to 18 French volunteers according to different protocols of immunization. Specific antibodies were detected in the three different fluids studied (saliva, jejunal fluid, serum) in one third of volunteers before vaccination. An increase of specific IgA antibody level was observed in jejunal fluids of most volunteers after oral vaccination. An augmentation of specific antibodies against vaccine antigenic components was also observed in serum and in saliva after vaccination but in fewer volunteers.

Administration, Oral↗
PubMed1987

[Mechanism of glomerular lesions in lupus erythematosus disseminatus].

Lupus glomerulonephritis is frequent and occurs in over 50% of patients with clinical evidence of systemic lupus erythematosus (SLE). It is generally accepted that lupus glomerulonephritis results from the deposition or the in situ formation of DNA/anti-DNA immune complexes in the kidney. However, circulating DNA/anti-DNA immune complexes have been found in only a minority of patients, no convincing data have been provided by experimental study in animals, and the demonstration of DNA in glomerular deposits remains questionable. Immunochemical properties of murine and human monoclonal anti-DNA antibodies suggest new pathogenic mechanisms for anti-DNA antibodies: their direct binding to a glomerular structure sharing an epitope with DNA. This hypothesis needs to be demonstrated in SLE patients and in experimental models.

Animals↗
PubMed1987

Effects of cyclosporine in severe systemic lupus erythematosus.

Thirteen patients with severe steroid-resistant or steroid-dependent forms of systemic lupus erythematosus were treated with cyclosporine (average dose 5 mg/kg/d) for an average period of 12 months. In eight patients the disease activity decreased, as substantiated by the reduction in the amount of steroid required to control the clinical manifestations. Interruption of cyclosporine treatment was associated with relapse or worsening of disease in five subjects. These favorable clinical results occurred in the absence of changes in the levels of antinuclear, anti-double-stranded deoxyribonucleic acid autoantibodies or plasma complement components; plasma IgG concentration increased significantly. Six patients had signs of moderate cyclosporine nephrotoxicity that disappeared when the administration of the drug was discontinued. Hypertension was the most serious side effect observed in eight subjects; in every case it was controlled by antihypertensive medicine. These data indicate that cyclosporine may be beneficial in the treatment of some patients with severe forms of systemic lupus erythematosus.

Adolescent↗
PubMed1986

Hepatitis B vaccination in chronic alcoholics.

Given the possible role of hepatitis B virus in the occurrence of hepatocellular carcinoma and the high prevalence of HBV infection in alcoholics, we attempted to prevent HBV infection in alcoholic patients with or without cirrhosis. Among 32 cirrhosis, 20 received three injections of hepatitis B vaccine at monthly intervals and 12 had a 4th injection one month later. Effectiveness was evaluated on the anti-HBs titer at the 6th month; it did not differ between the 3- and 4-injection groups. Two patients were good responders (anti-HBs greater than 300 mU/ml), 14 had a low response (m-30 mU/ml at the peak) and 16 (50%) had no detectable anti-HBs. All alcoholics without cirrhosis were given 4 injections; all had detectable anti-HBs but their mean antibody response was 97.7 +/- 4 SD. No obvious statistical difference in the response to the vaccine was noted on the basis of sex or age, or on the discontinuation or not of alcohol intake. Deficient antibody response to vaccines has not previously been demonstrated in patients with cirrhosis or in alcoholics. It remains to be determined whether this response is specific of HBV and how it could be related to the role of HBV in the occurrence of liver alterations in alcoholics.

Adult↗
PubMed1986

[Absence of in vivo binding of deoxyribonucleic acid to the glomerular basement membrane in C57BL/6 mice].

The intraaortic injection of radiolabeled deoxyribonucleic acid (DNA) into C57BL/6 mice treated with bacterial lipopolysaccharides 48 hrs. before, induced the renal deposition of DNA, as previously reported. Autoradiographic studies of the kidneys obtained from such mice did not demonstrate a selective binding of radiolabeled DNA to the glomerular basal membrane. These results argue against the in situ formation of DNA:anti-DNA immune complexes which is a proposed mechanism in the development of tissue lesions in the course of systemic lupus erythematosus.

Animals↗
PubMed1986

Idiotypes of monoclonal anti-DNA antibodies produced in autoimmune B/W mice are expressed in normal mice.

An anti-idiotypic antiserum was prepared in a rabbit immunized against a pool of six monoclonal anti-DNA antibodies generated in B/W mice. This antiserum detected idiotypic determinants in four of the six monoclonal anti-DNA antibodies but also in the serum of several non autoimmune strains (BALB/c, NZB X BALB/c) F1 hybrids & CBA/LH). The antiserum also reacted, but only to a weak degree, with B/W mouse sera. These results indicate that some idiotypes of anti-DNA antibodies produced by autoimmune B/W mice are present in normal mouse sera.

Animals↗
PubMed1985

Monoclonal anti-DNA antibodies: an approach to studying SLE nephritis.

Several data suggest that the glomerular deposits of DNA and anti-DNA antibodies observed in SLE result from complex formation in situ. The aim of this study was to investigate this hypothesis in normal C57BL/6 mice by using monoclonal anti-DNA antibodies (mAb). Renal localization of intravenously introduced ds DNA was demonstrated in mice injected intraperitoneally with LPS 48 h before. Then, a single IgG2b or a mixture of IgG2a and IgG2b anti-ds DNA mAb were given with the aim of forming DNA: anti-DNA complexes at the glomerular level. No immunoglobulin deposits were observed regardless of the antibody dose used. The mAb used may possess some of the qualitative properties suspected to be nephritogenic. Thus, the limiting factors in the induction of a passive nephritis could be either the absence of glomerular DNA deposits or the inability by using a single antigen-antibody system, to recreate the pathophysiological conditions seen in SLE, where a high number of antigen-antibody systems is implicated in the genesis, of glomerular lesions.

Animals↗
PubMed1984

A monoclonal anti-DNA antibody also binds to cell-surface protein(s).

A murine monoclonal anti-DNA antibody ( PME77 ) has been found to bind tightly to the plasma membrane of Raji cells. We show here that this monoclonal anti-DNA antibody reacts in a radioimmunoassay with the cell surface of a variety of mammalian cell types and that the antigenic determinant recognized by the monoclonal anti-DNA antibody at the surface of Raji cells is resistant to DNase. It belongs to polypeptides removed from the cell surface by a mild proteinase K treatment.

Animals↗
PubMed1984

Hepatitis B vaccine: clinical experience.

Hepatitis B virus (HBV) infections occur world-wide and more than 200 million people have been estimated to be chronic carriers of HB surface antigen (HBsAg). Long-term chronic carriage of HBsAg has been associated with an increased risk of chronic active hepatitis (CAH), cirrhosis and hepatocellular carcinoma (HCC). In Western countries, the prevalence of HBV infection is low in the general population. Only particular subgroups, for the most part adults, are at risk of such infections: health care workers, haemodialysis patients, transfusion patients, drug abusers and homosexuals. In Asia and tropical Africa, however, the prevalence of HBV infection is relatively high. Transmission occurs mainly during the perinatal period and infancy. Infection at a young age often results in the chronic carrier state. A hepatitis B vaccine has been developed in France and has been demonstrated to be safe, immunogenic, and effective in preventing HBV infection. Extensive experience from clinical trials now makes it possible to recommend vaccination strategies in terms of target populations and of optimal schedules. This paper reports the results of hepatitis B vaccination and, in particular, presents an overview of the vaccination experience in newborns and children.

Adolescent↗
PubMed1984

Induction of anti-DNA autoanti-idiotypic antibodies in (NZB X NZW)F1 mice: possible role for specific immune suppression.

(NZB X NZW)F1 (B/W) mice spontaneously produce anti-deoxyribonucleic (DNA) acid antibodies. PME77 anti-DNA monoclonal antibody (MoAb) is a syngeneic antibody bearing idiotype present in most B/W sera. In the present investigation the effect of immunization of B/W mice with the PME77 MoAb on the production of PME77 idiotypes and anti-DNA antibodies in B/W mouse sera was investigated. PME77 MoAb immunization regimen induced the production of autoanti-idiotypic antibodies and abrogated the expression of PME77 idiotype in B/W treated mice. In contrast, untreated mice and control B/W mice, receiving NZB polyclonal IgG2b which lacked detectable DNA binding capacity, expressed PME77 idiotopes. These results demonstrate that the expression of idiotype borne by autoantibodies may be modified through the induction of autoanti-idiotypic antibodies.

Animals↗