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Biomedical subjects

F W DENNY

Publications and source records attributed to F W DENNY.

At least 19 recordsLinked to original sources
PubMed1965

EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT).

The pathogenesis of Mycoplasma pneumoniae infection was studied in the Syrian hamster with qualitative and quantitative culture methods and special histopathologic techniques. The animals were readily infected with the mycoplasma, which multiplied throughout the respiratory tract. Sensitivity of this experimental host to infection was indicated by the 50 per cent infective dose, which was 10 colony-forming units of the organism. Inoculation consistently resulted in the production of peribronchial pneumonitis which was induced by the mycoplasma. The organisms were visualized in a superficial location in the mucosa of involved bronchi, by means of indirect fluorescent antibody staining and by a modification of the Brown and Brenn technique. The data indicate applicability of the hamster to the study of problems concerned with M. pneumoniae disease which are impractical or impossible to resolve in the human host.

Animals↗
PubMed1959

Sequential cellular changes produced by types 5 and 7 adenoviruses in HeLa cells and in human amniotic cells; cytological studies aided by fluorescein-labelled antibody.

The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. The evidence that is presented indicates that types 1, 2, and 5 belong to one major subdivision of the adenovirus group and types 3, 4, and 7 to another. That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. They have demonstrated the occurrence of type-specific adenovirus antigen in the characteristic intranuclear inclusions and other virus-induced structures reported to contain virus-like particles or shown by electronmicroscopy.

Adenoviridae↗
PubMed1959

Intracellular localization of type 4 adenovirus. I. Cellular fractionation studies.

HeLa cells infected with types 1 or 4 adenovirus were separated into cytoplasmic and nuclear fractions by mechanical disruption and differential centrifugation and the quantity of infectious virus in each was determined. The results showed that the majority of infectious virus of both types could be isolated in the cytoplasmic fraction. It was not possible to explain the large amount of type 4 virus in the cytoplasmic fraction by the number of nuclei disrupted in the fractionation procedure, but the amount of type 1 virus in the cytoplasmic fraction could have been contributed by disrupted nuclei. This suggested that there might be a basic difference in the intracellular formation of the two types of virus. The intracellular distribution of complement-fixing antigen was similar to that of infectious virus in type 4-infected cells. Technical difficulties, inherent in cellular fractionation studies, were encountered but did not appear to explain the results obtained.

Adenoviridae↗
PubMed1959

Intracellular localization of type 4 adenovirus. II. Cytological and fluorescein-labelled antibody studies.

HeLa cell cultures infected with adenovirus type 4 were studied by light and phase-contrast microscopy and by the fluorescent antibody technique for visualization of intracellular antigen. The findings were correlated with the growth curve of infectious virus, determined from companion cultures. The results indicated that those cells undergoing characteristic structural changes observable by light microscopy were those which contain viral antigen. The distribution of the majority of the antigen within the infected cells corresponded to that of the regularly aligned granules and crystal-like masses seen in the nuclei of cells in stained and in unfixed cultures. The production of infectious virus was closely correlated with the development of the characteristic nuclear changes.

Adenoviridae↗