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Biomedical subjects

F W Harrison

Publications and source records attributed to F W Harrison.

12 recordsLinked to original sources
PubMed1976

The fluorescent demonstration of tissue aldehydes with dansylhydrazine.

Dansylhydrazine, previously introduced in a selective fluorescent cytochemical method for the demonstration of sialic acid residues of cellular glycoconjungates, may be broadly applied as a specific, covalently bonded fluorochrome of aldehyde residues, both those naturally occurring as in elastin or those generated through PAS or Feulgen-type procedures.

Aldehydes↗
PubMed1975

The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates.

A new method for the fluorescent staining of stalic acid-containing glycoconjugates in fixed tissues is described. The procedure uses mild periodate oxidation, followed by condensation with dansylhydrazine and reduction of the hydrazones to hydrazines. The specificity of the reaction for sialic acid is tested on model glycoconjugates. The procedure gives superior resolution in comparison to the standard periodate Schiff procedure for cellular carbohydrates.

Animals↗
PubMed1975

Feulgen microspectrophotometric analysis of deoxyribonucleoprotein organization in larval and adult freshwater sponge nuclei.

Microspectrophotometric measurements of nuclei of adult choanocytes and archeocytes and larval archeocytes and flagellated ectodermal epithelia from stages III and IV were undertaken on tissues from the freshwater sponge, Eunapius fragilis (Leidy). The condensed nuclei of differentiated choanocytes and state IV flagellated epithelial cells presented integrated extinction values about 64% of those obtained for either adult or larval archeocytes. This suggests that such measurements represent sensitive indicators of deoxyribonucleoprotein-complex organization; thus, nuclear differentiation. Further, no large population of G2 (4C) nuclei was found among these populations as has been reported in two classes of coelenterates.

Animals↗
PubMed1975

Cytological examination of reduction bodies of Corvomeyenia carolinensis Harrison (porifera: spongillidae).

Freshwater sponges, Corvomeyenia carolinensis Harrison, were placed into tap water to induce degenerative reduction body formation. Reduction bodies were examined using light and electron microscopy in order to define their histochemical and ultrastructural characteristics. The reduction body of freshwater sponges is an extremely simple developmental system consisting primarily of an archeocyte reserve delimited by a simple squamous pinacoderm. The freshwater sponge reduction body displays many similarities to overwintering phases of marine sponges. The system presents an unusually straightforward vehicle for investigations of degeneration and regeneration as processes in developmental biology and may represent a reasonable vehicle in which to examine the process of the genesis of lysosomes.

Animals↗
PubMed1975

Fluorescent demonstration of basement membranes with hydroxy-naphthoic acid hydrazide.

The hydroxy-naphthoic acid hydrazide (HNAH) procedure can be employed not only for selective fluorochroming of side chain carboxyl groups of proteins, but also for demonstration of certain exoplasmic structures---particularly basement membranes. The demonstration of basement membranes by the HNAH procedure is likely related to the relatively high levels of available side chain carboxyl groups in the noncollagen glycoproteins of basement membranes.

Animals↗
PubMed1975

Evaluation of two protein end-group reactions as potential fluorescent cytochemical methods.

Histochemical preparations stained by a variant of the Morel-Sisley reaction for protein tyrosine were found to produce a red fluorescence when excited by broadband blue light which is topologically identical to the distribution of chromophore when viewed by absorption (equal transmission) microscopy. The fluorescence mode of viewing preparations stained by this method gave greater sensitivity and contrast than the absorption mode. The p-dimethylaminobenzaldehyde (DMAB)-nitrate method for protein tryptophan did not result in a useful fluorescent end-group method. Preparations stained by this method displayed a pattern of generalized fluorescence of all structures except those which react in the final step of the p-DMAB-nitrite reaction. The specificity of the intermediate reaction product has yet to be established.

Amphibians↗
PubMed1975

The cytology of the testaceous rhizopod Lesquereusia spiralis (Ehrenberg) Penard. I. Cytochemistry.

The cytology of the testaceous ameba, Lesquereusia spiralis (EHRENBERG) PENARD, has been described using a broad spectrum of cytochemical methods. Most cytological characteristics lie within the range of variability reported for free living non-shelled amebas. The shell of L. spiralis is complex, being composed of siliceous platelets positioned in an organic matrix. This interplatelet matrix is composed of sulfomucin complexed with basic protein. The matrix contains a complex of pore-like structures. The structure and possible origins and function of this matrix complex are discussed.

Amoeba↗
PubMed1975

Fluorescent cytochemistry of calid and algid normal and Lucké tumor-bearing kidneys.

Fluorescent cytochemical techniques, chiefly end-group methods, have been employed in the characterization of cells in calid and algid normal and LUCKE tumor-bearing kidneys of the leopard frog, Rana pipiens. Cytoplasmic RNA and glycogen distribution reflect the known patterns of general metabolic activity of the respective cells in question. Protein sulfhydryl distribution is similar in normal distal tubules and tumors while both differ significantly from proximal tubules. Viral inclusions in nuclei of algid tumor cells contain demonstrable DNA, protein sulfhydryls and disulfides, arginine and lysine-rich basic proteins, also both C-terminal and side chain carboxyl groups. Significant amounts of DNA are demonstrable in the cytoplasm of algid tumors. Fluorescence cytochemical methods directed toward end-groups or specific classes of macromolecules offer a particularly favorable option, because of increased sensitivity and higher levels of contrast, for comparative cytological investigations of the LUCKE tumor and of similar systems.

Adenocarcinoma↗