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Biomedical subjects

F W Wagner

Publications and source records attributed to F W Wagner.

At least 55 records · Page 3Linked to original sources
PubMed1980

Quantitative aerobic and anaerobic bacteriology of infected diabetic feet.

Quantitative aerobic and anaerobic cultures of deep tissue were performed on amputated infected lower limbs of 13 diabetic patients immediately after surgery. Dissection was made through intact skin distant from any preexisting ulcer. The results were compared with those obtained from: (i) ulcer swabs (pre- and postamputation), (iii) curettage of the ulcer base, and (iii) needle aspiration after normal saline injection. Anaerobic transport media were used for anaerobic cultures before prompt transfer to the anaerobic chamber. A mean of 4.7 bacterial species per specimen was seen (2.3 aerobes, 2.4 anaerobes). Mean log10 growth per gram of tissue was as follows: (i) aerobes plus anaerobes = 6.99, (ii) aerobes = 6.42, and (iii) anaerobes = 7.65. There was poor concordance between the deep tissue culture results and the results from other modalities of culture collection, though the results from other modalities of culture collection, though the results from curettage and saline aspiration were slightly better than those obtained from ulcer swabs. The most frequently isolated organisms were enterococci, anaerobic streptococci, and species of Proteus, Clostridium, and Bacteroides. When anti-microbial therapy is indicated for this patient population, the possibility of the concomitant presence of aerobes as well as anaerobes should be considered.

Aerobiosis↗
PubMed1979

Microbiology of superficial and deep tissues in infected diabetic gangrene.

In the current study, it is shown that, in the lesions of diabetic gangrene, there are usually mixed flora of two or more organisms. The organisms isolated from the presenting lesion may only partially reflect the organisms found in the deep tissues. Of the 58 cultures from the deep tissues, 11 of the isolates were anaerobes, but those anaerobes were never isolated as a pure culture. Bacterial synergism probably plays a role in infected diabetic gangrene. Maximizing the reduction of systemic infection and local cellulitis by elevation and appropriate antibiotic therapy results in improved wound healing in amputations of the lower extremities in patients with diabetic gangrene. However, apparent from this study, antibiotic therapy does not eradicate the organisms in the deep tissue, and surgical procedures are usually required for definitive treatment. Material from the surgically removed infected tissue should always be cultured for identification of existing organisms. When indicated, appropriate adjustment of antibiotic therapy, based upon these findings, should be prescribed.

Adult↗
PubMed1969

Growth of Chlorella sorokiniana at hyperbaric oxygen pressures.

The growth rate of Chlorella sorokiniana decreased in a linear fashion as the partial pressure of oxygen was increased from 711 to 1,478 mm of Hg. Under two atmospheres of oxygen pressure, growth ceased after 10 to 12 hr. This cessation of growth was not due to any permanent injury, as growth resumed when oxygen partial pressure was reduced to ambient levels. The inhibition occurred under both autotrophic and heterotrophic growth conditions and was not accompanied by an increase in cell size. The results indicated that the tolerance of Chlorella cells to elevated oxygen pressures was not an absolute immunity, and that inhibition of growth at very high oxygen pressures cannot be accounted for by an inhibition of photosynthesis alone.

Eukaryota↗
PubMed1969

Oxygen-tolerant strain of chlorella sorokiniana.

An oxygen-tolerant strain (OTS) of Chlorella sorokiniana was isolated by growing and continuously subculturing the oxygen-sensitive strain (OSS) in growth medium bubbled continuously with 95% O(2)-5% CO(2). Under these conditions, 6 to 8 hr were required before the OSS began to grow. The growth rates of the OTS and the OSS were the same when grown in an atmosphere of air-5% CO(2), and the growth rate of the OTS was the same when aerated with 95% O(2)-5% CO(2) and air-5% CO(2). The adaptive process was irreversible since serial transfers of the OTS grown with air-5% CO(2) did not alter the ability of the OTS to grow with 95% O(2)-5% CO(2). Inasmuch as photosynthesis in the OTS and the OSS was inhibited to a similar extent by oxygen and the adaptive process occurred heterotrophically in the absence of photosynthesis, it was concluded that the adaptive process was independent of photosynthesis. Morphological differences between the OTS and the OSS are presented and discussed with respect to the adaptive process.

Adaptation, Biological↗