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Biomedical subjects

F W Webb

Publications and source records attributed to F W Webb.

At least 19 recordsLinked to original sources
PubMed1986

The reduction of the number of animals used in the biological assay of insulin.

The biological control of insulin is vital as it is administered daily to a large proportion of the population during the major part of their lives. The development of the biological assay of insulin will be described with the progressive evolution of methods eventually to dispense with the need for animals. From the quantal Mouse Convulsion Method using around 600 mice per sample to the quantitative Mouse Blood Glucose method using an average of 120 mice per sample in which the fall in the blood glucose levels is used to estimate the potency of insulin by its hypoglycaemic effect. This was followed by the development of the in vitro Radioreceptor Method which is a procedure based on competition between the binding of insulin and [125I]-insulin to cells of the cultured human IM-9 cell line. The production of highly purified insulin by the Pharmaceutical Industry has made possible the recent introduction of new High Performance Liquid Chromatography technology.

Animal Testing Alternatives↗
PubMed1986

The "paper mouse".

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Animal Testing Alternatives↗
PubMed1975

Macrophage-lymphocyte clustering in rheumatoid arthritis.

The cells in synovial fluid from patients with rheumatoid arthritis contain a small percentage of macrophages. Such macrophages were isolated and cultured alone and with homologous and heterologous lymphocytes for 24 hours, in an attempt to identify possible contact between living lymphocytes and macrophages. Such contact was found, with clustering of lymphocytes around macrophages, and was particularly well shown by scanning electron microscopy.

Arthritis, Rheumatoid↗
PubMed1974

Calcinosis.

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Adult↗