PubMed Health⌕ Search

Biomedical subjects

F Wei

Publications and source records attributed to F Wei.

At least 55 records · Page 3Linked to original sources
PubMed1999

The Mla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley.

Powdery mildew of barley, caused by Erysiphe graminis f. sp. hordei, is a model system for investigating the mechanism of gene-for-gene interaction between large-genome cereals and obligate-fungal pathogens. A large number of loci that confer resistance to this disease are located on the short arm of chromosome 5(1H). The Mla resistance-gene cluster is positioned near the telomeric end of this chromosome arm. AFLP-, RAPD-, and RFLP-derived markers were used to saturate the Mla region in a high-resolution recombinant population segregating for the (Mla6 + Mla14) and (Mla13 + Ml-Ru3) resistance specificities. These tightly linked genetic markers were used to identify and develop a physical contig of YAC and BAC clones spanning the Mla cluster. Three distinct NBS-LRR resistance-gene homologue (RGH) families were revealed via computational analysis of low-pass and BAC-end sequence data derived from Mla-spanning clones. Genetic and physical mapping delimited the Mla-associated, NBS-LRR gene families to a 240-kb interval. Recombination within the RGH families was at least 10-fold less frequent than between markers directly adjacent to the Mla cluster.

Alleles↗
PubMed1999

[Determination of total sulfite in wine by high performance liquid chromatography(HPLC) with fluorescence detection].

A highly sensitive fluorometric method for the determination of total sulfite in wine is reported. The strong fluorescent derivatives formed by the quantitative reaction of sulfite in wine and N-(9-acridinyl) maleimide(NAM) in aqueous solution under mild conditions was injected into the HPLC. Separation was performed on an ODS column(200 mm x 5 mm) with a mobile phase of V(CH3CN):V(0.1 mo/L NH4AC) = 25:75 at a flow rate of 1.0 mL/min and detected by fluorometry at 455 nm(excitation at 365 nm). There was a good linear calibration curve in the range from 0.25-4 mg/L(r = 0.9998), The limit of detection was 1 ng. Sulfite mass concentration in wine has been determined by this method with good results and recoveries. This method is simple, rapid and reliable, and a sample size of only 1 mL is required to complete an analysis.

Chromatography, High Pressure Liquid↗
PubMed1998

Inflammation-induced Fos protein expression in the rat spinal cord is enhanced following dorsolateral or ventrolateral funiculus lesions.

Previous studies have shown an enhanced expression of Fos protein-like immunoreactivity in the lumbar spinal cord of rats with complete spinal transection following persistent hindpaw inflammation. To further locate the spinal pathways responsible for these effects, we compared the inflammation-evoked Fos expression in rats with bilateral lesions of the dorsolateral (DLFX) or ventrolateral (VLFX) funiculus, and with rats with a sham operation. The results indicate that the number of Fos-labeled neurons was significantly increased in all laminae of the dorsal horn ipsilateral to the inflamed hindpaw and in contralateral deep dorsal horn in both DLFX and VLFX rats compared to sham-operated rats. Moreover, when comparing DLFX and VLFX rats, in the ipsilateral spinal cord, DLFX resulted in more Fos expression in the deep dorsal horn; in contrast, a larger number of Fos-labeled cells in superficial laminae was observed in VLFX rats. These results suggest that modulatory systems, which descend in both DLF and VLF pathways, mediate the enhanced net descending nociceptive inhibition after persistent inflammation, although the supraspinal sites of origin of each pathway are likely functionally diverse.

Animals↗
PubMed1998

[Regeneration of costal cartilage by pectus excavatum and thoracic cage remodeling].

In order to identify whether the regeneration of costal cartilage is the basis of post-surgical repair of pectus excavatum and thoracic cage remodeling, 151 cases were followed up for 0.25 to 14 years. The main procedures in treatment were 3 steps: To curve the mental strut as a bow, to repair the perichondrium as a tube, and to persist in post-operative therapy. The results showed that regeneration of the costal cartilages appeared 3 months postoperatively in the cases treated by this method. It was concluded that a satisfactory thoracic cage could be remodeled by improving the technique of repairing pectus excavatum and persisting in postoperative therapy according to the regeneration regularity.

Adolescent↗
PubMed1998

[Esophageal reconstruction using ilecolon in children--a report of ten cases].

The use of the ileocolon as a substitute for esophagus in children has been a well-established surgical procedure. Between 1992 and 1995, ten retrosternal esophageal substitutions using an ileocolic interposition were performed. The ages of the children ranged from 2 to 8 years. The esophageal strictures were secondary to ingestion of caustic liquid. No death occurred in the intra- and post-operation period. Cervical ileoesophageal anastomotic leaks took place in 2 cases as the early major postoperative complications, which healed spontaneously. Three cases of ileoesophageal anastomotic strictures were noted in the follow-up period. The surgical indications, the treatments of anastomotic leaks and strictures, and the importance of long-term follow-up were discussed.

Burns, Chemical↗
PubMed1998

[Applied anatomy of the vascularity in the ileocolic region and its clinical significance].

To understand the characteristics of the patterns of arteries and veins distributed over the ileocolic segment, 50 cadavers were studied by gross-anatomy. The arteries, veins and their paracolic anastomoses distributed over ileocecal region, ascending colon and transverse colon were observed. The results showed that the distribution pattern of venous vessel was far more constant than that of artery, that the arrangements of artery in the ileocolic segment were classified into 7 types, and there were no interruption of paracolic anastomoses between arteries. But in 3 specimens the paracolic anastemoses between the right colic vein and the middle colic vein were completely interrupted. These findings may be useful in guiding clinical practice and preventing postoperative complications in ileocolic replacement of esophagus.

Adult↗
PubMed1997

Cell cycle-dependent colocalization of BARD1 and BRCA1 proteins in discrete nuclear domains.

Germ-line mutations of the BRCA1 gene predispose women to early-onset breast and ovarian cancer by compromising the gene's presumptive function as a tumor suppressor. Although the biochemical properties of BRCA1 polypeptides are not understood, their expression pattern and subcellular localization suggest a role in cell-cycle regulation. When resting cells are induced to proliferate, the steady-state levels of BRCA1 increase in late G1 and reach a maximum during S phase. Moreover, in S phase cells, BRCA1 polypeptides are hyperphosphorylated and accumulate into discrete subnuclear foci termed "BRCA1 nuclear dots." BRCA1 associates in vivo with a structurally related protein termed BARD1. Here we show that the steady-state levels of BARD1, unlike those of BRCA1, remain relatively constant during cell cycle progression. However, immunostaining revealed that BARD1 resides within BRCA1 nuclear dots during S phase of the cell cycle, but not during the G1 phase. Nevertheless, BARD1 polypeptides are found exclusively in the nuclear fractions of both G1- and S-phase cells. Therefore, progression to S phase is accompanied by the aggregation of nuclear BARD1 polypeptides into BRCA1 nuclear dots. This cell cycle-dependent colocalization of BARD1 and BRCA1 indicates a role for BARD1 in BRCA1-mediated tumor suppression.

BRCA1 Protein↗
PubMed1997

A contribution of neurokinin-1 receptor to formalin-induced c-fos expression in the rat spinal dorsal horn.

Formalin applied to a hindpaw of the rat resulted in c-fos expression in the spinal dorsal horn neurons on the ipsilateral side of stimulation. The role of neurokinin-1 (NK-1) receptor in this response was examined using a selective NK-1 antagonist L-668169. L-668169 administered intrathecally (1 microg/10 microl, 10 microg/10 microl) 15 min before injection of 2% formalin into one hindpaw significantly decreased the number of Fos-labeled neurons in the dorsal horn. A smaller dose of L-668169 (0.1 microg/10 microl) was ineffective. It was also showed that a selective NK-1 agonist Sar-SP perfused intrathecally (10 microg/10 microl) did result in the production of c-fos mainly in laminae I and that SP-like immunoreactive varicosities apposed neurons with Fos-labeled nuclei at their perikaryal membrane in the superficial laminae. These results indicate that NK-1 receptors may contribute to the activation of c-fos in the spinal dorsal horn neurons.

Animals↗
PubMed1997

Cross-leg free flaps for difficult cases of leg defects: indications, pitfalls, and long-term results.

BACKGROUND: Cross-leg free flaps have been described for reconstruction of leg defects in cases of trauma with no suitable recipient vessels in the vicinity of the defect. The pitfalls, however, have not been well documented in the literature. The technique of transfer and the length of time required before pedicle division depend on the nature of the flap and the contact surface. METHODS: Eight cross-leg free flap reconstructions were performed on seven patients between 1986 and 1993. These included three latissimus dorsi muscle flaps, three fibula osteomyocutaneous flaps, one tensor fascia lata myocutaneous flap, and one posterolateral thigh flap. The important technical considerations are outlined. RESULTS: One latissimus dorsi flap failed, whereas the rest of the flaps completely survived. The morbidity of the recipient and donor sites are listed. CONCLUSION: We conclude that the technique offers the possibility of salvaging limbs that are, otherwise, nonreconstructable. It is useful for young patients. When the flap is long, it can function as a nutrient flap for the distal limb even though the pedicle has been divided. It is a backup procedure in an urgent situation of re-exploration in which reestablishment of circulation should take place as soon as possible.

Adolescent↗
PubMed1997

St. Louis Integrated Immunization Information System.

The immunization rate for St. Louis, Missouri, children age two and younger is only 49 percent, below the statewide rate of 64 percent and far below the estimated national rate of 75 percent. In response to this crisis, the St. Louis Integrated Immunization Information System (SLIIIS) was designed to establish an on-line immunization information system, identify barriers to immunization among health care providers and the client population, and implement methods for removing these barriers. Available to both public and private health care providers, SLIIIS uses a population-based approach to monitoring and improving immunization levels in the Eastern Missouri Region. SLIIIS was initiated by a partnership of an academic center with community agencies and institutions. Review of initial development efforts and system design may be valuable to others involved in creating regionally integrated information systems.

Child, Preschool↗
PubMed1997

[An evaluation of the long-term results of palatopharyngeal closure function after velopharyngeal ring ligation].

16 cases were selected randomly from a group of 36 postoperative patients who underwent velopharyngeal ring ligation 16 years ago for evaluation of pronunciation, pharyngeal radiography, and electric endostroboskop to evaluate the patients' velopharygeal closure function. It was found that the pronunciation function of 93% patients were in good status. The results showed that centripetal force of velopharyngeal ring ligation made the velopharyngeal cavity reduced from the right to left and from anterior to posterior sides. It was good for sphincter of velopharyngeal moving, which could reduce the space of pharyngeal cavity and add the length of soft palate. The velopharyngeal closure was improved, which produced a good condition for the recovery of pronunciation function, at the same time, the change of pharyngeal cavity adapts to the patient's growth without limiting the growth and development of nasovelopharyngeal.

Adolescent↗
PubMed1997

[Studies on the chemical constitutens of Vicia amoena Fisch].

One new flavonoide was isolated from Vicia amoena Fisch. On the basis of spectral (UV, MS, NMR) and chemical reactions, it was elucidated to be kaempferol-3-O-beta-D-mannoside, named amoenin(A3). Moreover, five known compounds have been isolated and identified as quercetin, kaempferol, quercetin-3-O-alpha-L-rhamoside, quercetin-3-O-beta-D-glucoside, kaempferol-3, 7-O-alpha-L-dirhamoside. The total flavonoides showed significant effects on inducing hyperlipidemia and increasing micro-blood vessel elasticity.

Drugs, Chinese Herbal↗
PubMed1997

[Study on relationship of soluble interleukin-2 receptors in serious hepatitis B patients and the T lymphocyte subgroup].

The assay of sIL-2R and T lymphocyte subgroup in 21 cases of serious HB patients revealed that serum sIL-2R in patients of acute and subacute or chronic serious HB was significantly higher than those in normal controls, P<0.001, but the CD4+ lymphocytes in all these patients obviously decreased and CD8+ lymphocytes notably increased when compared with that in normal group, P<0.01. The results showed that increasing of sIL-2R is associated with the activation of CD8+ cells which is mainly of cytotoxicity T lymphoeytes that play an important role in the pathogenesis of acute and subacute serious HB. The different expression of CD4+ and CD8+ in acute and chronic serious HB suggests a different pathogenetic mechanism between them.

Adult↗
PubMed1997

[Determination of aniline derivatives in waste water with reversed-phase high performance liquid chromatography].

A reversed-phase high performance liquid chromatographic method for the determination of aniline derivatives in waste-water is described. Chromatographic conditions were: column, Hypersil BDS (200 mm x 4.0 mm i.d., 5 microm); mobile phase, methanol-water, gradient elution; flow rate, 1.0 mL/min; detection wavelength, 254 nm. Quantification was carried out based on the external standard of aniline derivatives. The pH of waste water sample was adjusted to 11-12 with dilute NaOH solution prior to extraction with CH2Cl2. The extractives concentrated in K-D concentrator and filtered with FH 0.5 microm membrane. Finally make up the volume to 1 mL and test it with reversed-phase high performance liquid chromatography. The linearity range of calibration curve used was 0.005-0.3 microg. The minimum detectable quantities of aniline derivatives varied from 1.20 ng to 3.00 ng. The average recoveries were from 51.57% to 107.92%, and the RSDs of the method were from 1.88% to 5.98%. The method is simple, rapid, and accurate.

Aniline Compounds↗
PubMed1997

Sex hormone receptors of hemangiomas in children.

OBJECTIVE: To investigate whether or not estrogen receptor and other sex hormone receptors are present in hemangioma tissues of children. METHODS: Fifty-two specimens of hemangiomas were taken from 52 children. The specimens were doubly stained with hematoxylin-eosin and enzyme-linking affinity immunohistochemistry. The prepared tissue slides were observed under the microscope to search for estrogen receptors (ER), progestogen receptors (PR) and androgen receptors (AR) in all the cells. RESULTS: ER, PR and AR were detected in various kinds of hemangiomas. The mean positive cell rate of each sex hormone receptor in different hemangioma tissues revealed no significant distinction in statistics (P > 0.05). CONCLUSIONS: The results suggest that hemangiomas are one of the target tissues of estrogen. Estrogen, ER, PR, and AR may play an important role in the growth and development of hemangiomas.

Adolescent↗
PubMed1997

[Fetal fibronectin and preterm birth].

OBJECTIVE: To determine whether fetal fibronectin is a sensitive test for the diagnosis and prediction of preterm birth. METHODS: Fetal fibronectin was measured by enzyme-labeled immunosorbent assay (ELISA) methods in sample of cervical secretion from 38 women with threatened preterm delivery (study group) and 43 normal pregnanty women with same gestational weeks (control group). RESULTS: The positive rate of fetal fibronectin in women with threatened preterm birth was significantly higher than that in the normal control group. CONCLUSION: Fetal fibronectin is an important marker. It can be used for diagnosis and prediction of preterm birth.

Adult↗
PubMed1996

Blockade of capsaicin-induced reduction of GABA-immunoreactivity by spantide in cat spinal superficial dorsal horn.

In our previous study, perineural application of capsaicin not only produced release and depletion of substance P from primary nociceptive afferent terminals, but also reduced GABA immunoreactivity in the superficial dorsal horn. The aim of the present study was to determine whether the release of GABA is triggered by substance P released from primary nociceptive afferent terminals by capsaican. GABA and substance P immunoreactivity in the lumbar dorsal horn was examined in two groups: in the first group the tibial nerve was treated with 3% capsaicin, and in the second group the dorsal surface of the lumbar cord was infused with spantide (50 nM), a substance P receptor antagonist, before application of capsaicin to the tibial nerve. Following perineural treatment of capsaicin for 30 min, both the GABA-immunostaining density and the number of GABA immunoreactive neurons were reduced significantly in the ipsilateral laminae I-II at L5 through L7. GABA immunoreactivity was reduced by 54.12%, 44.46% and 31.0% in the medial, central and lateral parts of the ipsilateral laminae I-II at L7, respectively. With pre-application of spantide to the spinal cord, GABA immunoreactivity was reduced only to 14.4%, 16.4% and 10.16%, respectively, in the medial, central and lateral parts of laminae I-II at L7 and no reduction of GABA immunoreactive neurons was observed. Additionally, capsaicin-induced reduction of substance P immunoreactivity was partially blocked by spantide. These results suggest that capsaicin produces substance P release from primary nociceptive afferent terminals, and that substance P, in turn, activates the second-order GABAergic interneurons in the dorsal horn. The functional significance of capsaicin-induced activation of GABAergic neurons in modulation of spinal nociception is discussed.

Analgesics↗