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Biomedical subjects

F Wisløff

Publications and source records attributed to F Wisløff.

At least 73 records · Page 4Linked to original sources
PubMed1989

[Recurrence or therapeutic resistance of acute myelogenous leukemia. Effect of mitoxantrone and cytosine arabinoside].

Adult patients with relapsed and refractory acute myelogenous leukemia usually demand further therapy aimed at achieving remission. The physician knows that life expectancy is limited. Care must be taken to choose a cytotoxic regimen where the potential gains outweigh the risks. Mitoxantrone and cytosine arabinoside were given in combination to 19 patients. Six achieved complete remission and increased survival. The side effects were acceptable.

Adolescent↗
PubMed1989

[Splenectomy in hematologic diseases].

We present the results of splenectomy in 45 patients with haematological diseases during the period 1975-85. Of 16 patients with immunologic thrombocytopenia, splenectomy resulted in a satisfactory and sustained platelet elevation in 11 (69%). Splenectomy corrected anaemia in all five patients with hereditary spherocytosis. Of seven patients with autoimmunohaemolytic anaemia, four responded satisfactorily. Postoperative complications occurred in ten of 45 patients (22%). There was no post-operative mortality. Only 49 per cent of the patients had received antipneumococcal vaccination. This is not satisfactory, and all unvaccinated, splenectomized patients should be offered vaccine. If possible, the vaccination should be performed before operation.

Adolescent↗
PubMed1987

Monoclonal IgM with lupus anticoagulant activity in a case of Waldenström's macroglobulinaemia.

Strong lupus anticoagulant activity was detected in the plasma of a 73-yr-old woman with Waldenström's macroglobulinaemia. Purified patient IgM caused significant prolongation of the APTT in concentrations as low as 30 micrograms/ml, and its activity was inhibited by intact or (more effectively) by frozen-thawed blood platelets. Employing varying concentrations of cephalin in the APTT assay, it was demonstrated that the macroglobulin had a direct cephalin-neutralizing effect. When tested in an ELISA system using phospholipid-coated microtiter wells, the IgM was shown to possess antibody activity against cephalin. With purified phospholipids as antigens, the protein was found to display antibody activity against phosphatidyl serine and phosphatidyl ethanolamine, which are main constituents of cephalin. These observations were confirmed in absorption experiments.

Aged↗
PubMed1985

The molecular localization of the ability of certain monoclonal immunoglobulins to interfere with fibrin polymerization.

When a purified myeloma protein with the ability to inhibit fibrin polymerization was preincubated with sheep anti-human F(ab')2 antibodies, there was a marked reduction of the ability to prolong the thrombin clotting time, while anti-Fc antibodies had only a slight effect. A monoclonal immunoglobulin which shortens the thrombin time was uninfluenced by anti-F (ab')2 as well as by anti-Fc antibodies. Partial reduction and alkylation of the immunoglobulins did not modify their effect on the thrombin time. None of the immunoglobulins bound to fibrinogen or fibrin monomer that had been linked to CNBr-activated agarose. Myeloma proteins may interfere with fibrin polymerization in two ways: some proteins inhibit polymerization in a reaction that depends on the Fab (antigen binding) part of the molecule; however, antibody binding activity against fibrinogen or fibrin monomer has not been demonstrated. Other (rare) myeloma proteins accelerate fibrin polymerization; this effect cannot be ascribed to any particular portion of the molecule and is probably of an unspecific nature.

Animals↗
PubMed1985

Effect of high-dose ampicillin and cloxacillin on bleeding time and bleeding in open-heart surgery.

To determine if platelet dysfunction caused by high doses of penicillin compounds is of practical importance in patients with additional haemostatic defects perioperatively, a study was made of patients undergoing open-heart surgery. They were randomly assigned to prophylactic treatment with ampicillin 8 g plus cloxacillin 4 g daily for three days, or with cephalothin 8 g daily for three days. Fifty patients in each group were evaluated. The median bleeding time preoperatively and on days 1 and 4 postoperatively did not differ between the groups. The bleeding time was prolonged beyond the normal range in eight patients of the ampicillin/cloxacillin group and in three of the cephalothin group (p less than 0.05). Prolonged bleeding time was not associated with lower platelet count or greater blood loss. The total blood loss and the amounts of transfused blood, platelets and cryoprecipitate were all greater in the ampicillin/cloxacillin group, but the difference was not statistically significant. Combined use of ampicillin and cloxacillin in open-heart surgery is associated with increased bleeding, but the increase is without practical importance.

Adolescent↗
PubMed1984

Inhibition or acceleration of fibrin polymerization by monoclonal immunoglobulins and immunoglobulin fragments.

A prolongation of the thrombin clotting time by at least 25% was found in the presence of 8 of 26 IgG myeloma proteins, 2 of 5 IgA proteins and 1 of 10 macroglobulins, in a concentration of 10 mg/ml. Normal pooled immunoglobulin in concentrations up to 83 mg/ml were inactive. 2 IgG and 2 IgM monoclonal immunoglobulins caused an acceleration of the thrombin time by at least 20%; this phenomenon has not been reported before. Corresponding results were obtained with these proteins when the polymerization of des-AA and des-AABB fibrin monomers were investigated in a light scattering system. Studies with enzymatically produced Fab, F(ab')2 and Fc fragments from normal and monoclonal immunoglobulin suggested that the polymerization inhibitory activity resides in the Fab portion of the immunoglobulin molecule. While the ability to inhibit fibrin polymerization may be an immunoglobulin specific effect, the acceleration caused by certain other monoclonal immunoglobulins may be an unspecific effect similar to that seen with dextran or albumin.

Animals↗
PubMed1983

Sézary cells with an unusual phenotype, their modulation with 12-O-tetradecanoyl phorbol-13-acetate (TPA) in vitro, and their relationship to T-cell development.

Leukemic cells from a patient with Sézary syndrome were studied with a panel of monoclonal antibodies. Despite their typical morphology, the cells expressed an unusual surface phenotype. They simultaneously expressed OKT4 and OKT8 reactivity, indicating a more immature origin of the neoplastic cells than what has previously been observed in Sézary syndrome, but they were OKT6 negative. In response to 12-O-tetradecanoyl phorbol-13-acetate (TPA) the cells underwent phenotypic alterations, as the OKT4 reactivity was significantly reduced and the OKT8 expression was retained. Thus, following TPA, the cells expressed a surface phenotype consistent with the cytotoxic/suppressor T-cell subset in normal peripheral blood. The findings are discussed in relation to normal T-cell maturation and differentiation processes.

Aged↗
PubMed1983

Effect of prophylactic high-dose treatment with ampicillin and cloxacillin on bleeding time and bleeding in patients undergoing elective vascular surgery.

50 patients undergoing elective vascular surgery were randomized to prophylactic antibiotic treatment with ampicillin 2 g X 4 + cloxacillin 1 g X 4 for 4 d following the operation, or to a control group without antibiotics. All patients received heparin subcutaneously 5000 IU X 2, for 7 d. On days 1 and 4 after the operation, the median bleeding time was 1 1/2-2 min longer in the antibiotics group. 5 patients on antibiotics had a bleeding time prolongation beyond the normal range, but only 2 of these, who had additional haemostatic defects, had abnormal bleeding.

Adult↗