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Biomedical subjects

F Wolter

Publications and source records attributed to F Wolter.

At least 19 recordsLinked to original sources
PubMed2001

Downregulation of the cyclin D1/Cdk4 complex occurs during resveratrol-induced cell cycle arrest in colon cancer cell lines.

Resveratrol is a naturally occurring polyphenol with cancer chemopreventive properties. The objective of the current study was to investigate the effect of resveratrol on the human colonic adenocarcinoma cell line Caco-2. The compound inhibited cell growth and proliferation of Caco-2 cells in a dose-dependent manner (12.5-200 micromol/L) as assessed by crystal violet assay, [(3)H]thymidine and [(14)C]leucine incorporation. Furthermore, apoptosis was determined by measuring caspase-3 activity, which increased significantly after 24 and 48 h of treatment with 200 micromol/L resveratrol. Perturbed cell cycle progression from the S to G2 phase was observed for concentrations up to 50 micromol/L, whereas higher concentrations led to reversal of the S phase arrest. These effects were specific for resveratrol; they were not observed after incubation with the stilbene analogs stilbenemethanol and rhapontin. Levels of cyclin D1 and cyclin-dependent kinase (cdk) 4 proteins were decreased, as revealed by immunoblotting. In addition, resveratrol enhanced the expression of cyclin E and cyclin A. The protein levels of cdk2, cdk6 and proliferating cell nuclear antigen were unaffected. Similar results were obtained for the colon carcinoma cell line HCT-116, indicating that cell cycle inhibition by resveratrol is independent of cyclooxygenase inhibition. The phosphorylation state of the retinoblastoma protein in Caco-2 cells was shifted from hyperphosphorylated to hypophosphorylated at 200 micromol/L, which may account for reversal of the S phase block at concentrations exceeding 50 micromol/L. These findings suggest that resveratrol exerts chemopreventive effects on colonic cancer cells by inhibition of the cell cycle.

Alkaline Phosphatase↗
PubMed1998

[Value of ultrasound in diagnosis of appendicitis].

The diagnostics of acute appendicitis is also a challenge for a surgeon today. The question of suitable, supplement diagnostics methods is topical. Experience with percutan sonography in the hand of surgeon in one-year-study are demonstrated and possibilities, problems and views should be to show. In skilled hand the sonography is an easy, little burden and helpful method to determinate right lower pain.

Abdomen, Acute↗
PubMed1990

Neurophysiological effects of substance P in primate hypertension models. Preliminary report.

Peripheral administration of the neuropeptide Substance P (SP) in rhesus monkeys and baboons caused a long lasting decrease in blood pressure, accompanied by a gradual normalization function of stress-induced bioelectrical activity changes in brain structures, relevant for cardiovascular regulation (frontal cortex, ncl. raphe, locus coeruleus). In addition, SP was found to normalize the disturbed interaction of brain structures of both hemispheres. Use of SP as a putative agent to treat essential hypertension in humans needs further studies.

Animals↗
PubMed1981

[Control of veterinary communicable diseases in swine and fattening cattle stocks by serological testing of slaughtered animal blood samples].

Economy of prophylactic haemoserological control of pig stocks in a district as well as of a sow breeding unit and of cattle stocks on two industrialised fattening farms has been enhanced by including blood samples from routine slaughtering. Preliminary organisational experience is reported in this paper. The samples collected samples collected from normally slaughtered selected sows may contribute to an improvement of veterinary production control.

Abattoirs↗
PubMed1976

[Neurophysiologic mechanisms of arterial hypertension under experimental chronic emotional stress].

Neurophysiological studies were conducted with subhuman primates (macaca mulatta) in order to obtain an estimate of central nervous effects of socio-emotional stress. This was combined with continuously aggravated conditioning procedures in view of the possible significance of chronic environmental stress escalation for etiology and pathogenesis of an arterial hypertension model. Our conclusions are based on evoked potentials (EP) as integrative characteristics of cerebral information processing. The EPs were recorded by means of electrodes chronically implanted in brain structures of emotional and cardio-vascular relevance. Multivariate mathematico-statistical analyses of average EPs (AEP) provide an objective measure of stress sensibility of the individual, particularly of the effects of acute and chronic environmental stress factors upon the functional organization of the CNS. By means of a quantitative approach to AEP we were able to demonstrate a disjunction between distinct limbic and hypothalamic structures starting under stress conditions of subchronic character. We assume that the constancy of functionally antagonistic hyperactive excitation foci at diencephalic and supradiencephalic levels and their specific interaction with the equally stress related neocortical functional insufficiency constitutes a decisive pathogenetic central mechanism of neurotic behaviour. Long-term changes of amplification of external and internal afferences could be demonstrated on the basis of hypo- and hyperreactive neuroelectric functional patterns. These processes cause cerebro-visceral regulatory diseases as, e. g., a primary arterial hypertension by restriction of neocortical control and the corresponding efferent reactions for re-establishment of the dynamic homeostasis.

Animals↗
PubMed1976

[Changes in blood pressure, heart rate, respiration frequency and ECG in rhesus monkeys exposed to socio-emotional stress].

Blood pressure, heart rate, respiration frequency and ECG parameters were measured in 3 juvenile male rhesus monkeys under acute and chronic influence of a stepped load schedule including positive (nutritional), negative (pain-related) and socially operant conditioning and phases of a pharmacological sensibilization (angiotensin-II) of the cardiovascular system. The results suggest a general adaptation to the positively operant conditioning, while the latter stressing influences (either single or in combination) produced blood pressure and heart rate elevations, which had the character of dysregulatory functional changes (borderline hypertension model) and were reached by other authors, if at all, only through stronger stress influences.

Animals↗
PubMed1976

[The vaginal transverse low segment hysterotomy for second trimester therapeutic abortion (author's transl)].

A series of 100 therapeutic abortions by vaginal hysterotomy is reported. This method of vaginal hysterotomy with a transverse low segment incision just above the internal os of the cervix was first described by Fuchs in 1939. The important advantage of this method is complete avoidance of the uterine cervix. With this method a therapeutic abortion after the tenth week of gestation and in a few cases, up to the 20th week of gestation can be accomplished by a fast one stage technique with few side effects. The method is considered to be superior to the injection methods and the two stage procedures. With skill in vaginal operative techniques, this method can be used in any gynaecological department as a routine method. In this series, 28 cases had concomitant tubal ligations. There were two subsequent pregnancies. Complications were one uterine perforation during the curettage requiring laparotomy and suture. There was one laceration of the trigon of the bladder requiring suture. There were 2 cases of hemorrhage. There were 6 inflammatory reactions in the perimetria of which two required administration of antibiotics. There was superficial thrombophlebitis in one case. And there was a severe coagulation defect in one case of a missed abortion at 5 months gestation.

Abortion, Induced↗
PubMed1975

Impacts of environmental stress exposure on central nervous functional patterns. A quantitative approach to evoked potential data.

Albino rats and rhesus monkeys with chronically implanted electrodes in cortical and subcortical, vasomotory and emotionally relevant brain regions were subjected to psycho-nerval stress (short-term stressful CR learning experiments on rats, long-term environmental stress with disturbances of the diurnal rhythm and social hierarchy on rhesus monkeys). Applying average computer techniques and discriminance analyses to evoked potentials (average evoked potentials = AEP to standardized optic-acoustic test stimuli) we were able to objectify the effect of different stress categories on central nervous functional patterns. These phenomena of electrical activity of the CNS proved to be extremely responsive to the stress exposure (avoidance conditioned learning experiments or socio-emotional overstrain). Under moderated stress (CR phases of acquisition and stabilization), time-space patterns of a primary facilitation of AEP could be established, expressing an improved energetic capacity of the CNS (adaptive effect). On the other hand, there was a generalized impairment of bioelectric information processing in temporal correlation with an increase in psycho-nervous stress exposure to rats (CR phases of differentiation). During long-term socio-emotional stress exposure of rhesus monkeys the highly significant changes of AEP-patterns (increasing fall short of the set point of the bioelectric level of activity with segmental differences in the multiphasic evoked potential) are to be interpreted as maladaptive processes in central nervous function under long lasting high stress conditions.

Adaptation, Physiological↗