Problems in primary care.
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Internal Medicine↗
Biomedical subjects
F X Kieliszek
Publications and source records attributed to F X Kieliszek.
A radioimmunoassay for 13, 14-dihydro-15-keto prostaglandin F2alpha with chromatography and internal recovery standard.
Antibodies to the 13, 14-dihydro-15-keto metabolite of prostaglandin F2alpha(PGF2alphaM) were raised in sheep using a bovine thyroglobulin conjugate of PGF2alphaM. Labeled 13, 14-dihydro-15-keto prostaglandin A2(PGA2M), 13, 14-dihydro-15-keto prostaglandin E2 (PGE2M) and PGF2alphaM were prepared from their corresponding high specific activity parent prostaglandins with swine kidney homogenate and purified using reverse phase liquid-liquid partition chromatography. A rapid method of column chromatography for use prior to radioimmunnoassay was developed. Mathematical corrections for the effect of recovery tracer on the logit/log transformation are presented with a new approach to expression of radioimmunoassay cross reactions allowing continuous expression of the variation of these cross reactions with displacement. These mathematical approaches are widely applicable to other radioimmunoassay systems and transformations. The assay was used for measurement in groups of human volunteers: males, females, women at delivery and paired venous umbilical cord bloods. A correlation between venous cord and maternal peripheral PGF2alphaM levels is demonstrated with a gradient from the cord plasma to maternal plasma.
Antibody Formation↗
Uterine artery blood flow studies in the pregnant monkey.
Studies were performed in anesthetized monkeys in the third trimester of pregnancy, with continuous monitoring of maternal blood pressure and uterine artery blood flow by electromagnetic flow probe and with measurement of prostaglandin levels in uterine vein samples. Acute elevation of the systemic blood pressure by intravenous infusion of angiotensin II was associated with increased uterine artery blood flow and an increase in the levels of prostaglandin E measured in the uterine vein. An increase in PGE levels was also observed following the discontinuation of the angiotensin II infusion, when blood pressure and uterine artery blood flow returned to baseline levels. Intravenous administration of indomethacin was associated with a slight rise in maternal blood pressure. The changes in uterine artery blood flow following indomethacin treatment were variable, perhaps reflecting the stresses of surgery and anesthesia. In single experiments, intravenous diazoxide produced a profound hypotension, and intravenous furosemide induced a prompt and marked diuresis, associated with a very marked fall in uterine artery blood flow.
Angiotensin II↗