Biomedical subjects

Fanghui Liang

Publications and source records attributed to Fanghui Liang.

3 recordsLinked to original sources

PubMed2006

Investigation on hyperin-cetyltrimethylammonium bromide-fibronectin system by resonance light-scattering technique.

A simple, highly sensitive assay for fibronectin (Fn) was reported using resonance light-scattering (RLS) technique based on the enhanced RLS intensity of hyperin-cetyltrimethylammonium bromide (CTMAB)-Fn system. The interaction system of hyperin-CTMAB-Fn was investigated using spectral methods. Mechanistic investigations show that the main reason of the enhanced RLS intensity of Fn is the formation of three-component complex (hyperin-CTMAB-Fn), in which CTMAB acts as a bridge between hyperin and Fn. The effects of pH, surfactant, concentration of CTMAB and hyperin, incubation time and foreign substances on the enhancement of RLS intensity were studied. Under the optimum conditions, the enhancement of RLS intensity is in proportion to the concentration of Fn in the range of 1.9-248ng/ml. The synthetic samples containing Fn were analyzed and results obtained were satisfied.

Cetrimonium↗

PubMed2006

Investigation on the interaction between colloidal gold and human complement factor 4 at different pH by spectral methods.

The interaction between colloidal gold and human complement factor 4 (human C4) at different pH was investigated by spectral methods, including absorption and resonance light-scattering spectrometry. According to the changes of color and absorption spectra of colloidal gold solution in presence of human C4, the interaction between colloidal gold and human C4 was quantitatively investigated using a semi-empirical "flocculation parameter". At the same time, the changes of resonance light-scattering spectra and transmission electron microscopy (TEM) images indicate that the aggregation of colloidal gold happens by electrostatic interaction in presence of human C4 in the pH range 5-6. However, the colloidal gold solution remains stable at pH >6 and pH <5 due to the repulsive electrostatic interaction between colloidal gold and human C4. The flocculation parameter is directly proportional to the concentration of human C4 in the range from 9.7 to 233.0 microgl(-1). In addition, the interactions between the colloidal gold and bovine serum albumin (BSA) as well as human serum albumin (HSA) were also investigated using the same methods. It was found that there was no aggregation of colloidal gold in presence of BSA and HSA in the pH range 5-6. However, when the pH of solution is 4, the aggregation of colloidal gold happens. Because BSA and HSA have different structure, the intensity of aggregation of colloidal gold in presence of BSA is greater than that in presence of HSA at pH 4.

Animals↗

PubMed2005

Determination of human complement factor C4 using resonance light-scattering technique with sodium dodecylbenzene sulphonate probe.

Based on the interaction between human complement factor C4 (human C4) and sodium dodecylbenzene sulphonate (SDBS) and the resonance light-scattering (RLS) technique, a highly sensitive assay for human C4 using resonance light-scattering technique was developed. At pH 2.8 Na2HPO4-citric acid buffer solution, the RLS intensities of SDBS system at 283, 503 and 600 nm were obviously enhanced in the presence of human C4. The effects of surfactant, pH, incubation time, concentration of SDBS and foreign substances on the enhanced RLS intensity of system were investigated. Under the optimum conditions, the enhanced RLS intensity is directly proportional to the concentration of human C4 in the range of (0.5-120)x10(-6)gl-1 and the linear regression equation was obtained with high correlation coefficient. This RLS technique was applied to the determination of human C4 in some synthetic samples with good recovery. Moreover, it was found that the electrostatic interaction is the main binding force between SDBS and human C4.

Benzenesulfonates↗