Biomedical subjects

Fei Zhou

Publications and source records attributed to Fei Zhou.

22 records · Page 2Linked to original sources

PubMed2003

[Therapeutic efficacy of combined application of lamivudine and bushen recipe in treating chronic hepatitis B and its influence on YMDD motif].

OBJECTIVE: To observe the effect of combined application of Lamivudine and Bushen recipe (BSR) in treating patients with chronic hepatitis B (CHB) and its influence on tyrosine-methionine-aspartate-aspartate (YMDD) motif. METHODS: Eighty-eight patients of CHB with positive HBV-DNA were divided into 3 groups, Group A treated with Lamivudine, Group B treated with Lamivudine plus BSR1 and Group C treated with Lamivudine plus BSR2, the serum HBV-DNA, hepatic biological parameters and YMDD motif mutation were observed. RESULTS: The positive rate of HBV-DNA (6.45%) and YMDD motif mutation (3.23%) in Group C were lower than those in Group A respectively, after 52 weeks treatment. CONCLUSION: Combined application of Lamivudine and BSR could enhance the therapeutic effect in treating CHB to certain extent, and could reduce the mutation of YMDD motif.

Adolescent↗

PubMed2002

Construction of the plasmid pCMV4-rZPC' DNA vaccine and analysis of its contraceptive potential.

Zona pellucida C (ZPC) is a major glycoprotein of the zona pellucida that possesses the sperm receptor function. ZPC induces autoantibody that can block sperm/oocyte interaction. We selected the partial sequence of rabbit ZPC (amino acid 263-415, rZPC') as the target and constructed the pCMV4-rZPC' gene vaccine by using DNA recombinant techniques. The total RNA was extracted from the ovaries of the sexually healthy female rabbit, and the rZPC' cDNA, which was amplified by reverse transcription-polymerase chain reaction, was directly inserted into the cloning vector PCR2.1 to construct the PCR2.1-rZPC'. This insertion fragment was subcloned into the pCMV4 vector to form the pCMV4-rZPC' prototype DNA vaccine. All experimental BALB/C mice and New Zealand rabbits received i.m. injection of pCMV4-rZPC' vaccine three times. The results show that 1) the pCMV4-rZPC' construct expresses rZPC' cDNA in mice muscle cells, 2) 60% of the immunized female mice were infertile at 6 wk after the immunization, 3) the mice immunized with pCMV4-rZPC' DNA vaccine developed anti-rZPC antibodies that bound to the ovarian ZP in situ, and 4) antibodies against rZPC' were also bound to normal animal ovarian ZP in vitro. The results indicate that anti-rZPC antibodies developed from pCMV4-rZPC' DNA vaccine can prevent the fertility course and do not interfere with normal follicular development. The pCMV4-rZPC' DNA vaccine may be possible to develop as a contraceptive vaccine.

Animals↗

PubMed2002

Spinor bosonic atoms in optical lattices: symmetry breaking and fractionalization.

We study superfluid and Mott insulator phases of cold spin-1 Bose atoms with antiferromagnetic interactions in an optical lattice, including a usual polar condensate phase, a condensate of singlet pairs, a crystal spin nematic phase, and a spin singlet crystal phase. We suggest a possibility of exotic fractionalized phases of spinor Bose-Einstein condensates and discuss them in the language of Z2 lattice gauge theory.

Journal Article↗

PubMed2002

[Chromosomal localization of rice field eel Hox genes by PRINS].

The genome duplication and chromosome rearrangement are two kinds of evolution models at the chromosome level during the evolution of vertebrate genome. And Hox genes are the powerful proves to support the evolution theory of genome duplication, which has been found recently. In this study, the chromosomal localization of rice field eel Hox genes has been carried out by PRINS. The mapping results indicated that 6 Hox clusters might exist in rice field eel genome, and these clusters were localized on chromosome 1, 2, 3, 6, 8, 10 and at the position of 28.24 +/- 2.88, 4.55 +/- 1.39, 13.89 +/- 2.03, 74.32 +/- 1.86, 38.03 +/- 2.41, 58.18 +/- 2.05 from the centromere respectively. The mapping results that Hox genes were localized on chromosome 1, 3, 6 and 10 in the study are corresponding to that by chromosome microdissection. The chromosomal localization of rice field eel Hox genes will help us to discover the origin and evolution of rice field eel chromosomes, and provide cellular genetic proves of this special species to support the evolution theory of genome duplication.

Animals↗