Biomedical subjects

Frédéric Padilla

Publications and source records attributed to Frédéric Padilla.

7 recordsLinked to original sources

PubMed2006

Experimental evaluation of bone quality measuring speed of sound in cadaver mandibles.

OBJECTIVES: To demonstrate in vitro the feasibility of speed of sound (SOS) measurements through the mandible and to investigate the relationships between mandibular SOS, local bone mineral density (BMD), and the ratio between trabecular and cortical thicknesses (Tb.Th/Cort.Th). The long-term goal is to find a safe, simple test for bone quality in sites for dental implant placement. METHODS: Excised human mandibles (N = 23) were used for the measurement of sound transmission with two 1.6 MHz transducers. Three regions of interest (ROIs) were selected in each of the specimens, namely incisor, premolar, and molar regions. To determine short-term precision, 10 measurements (with repositioning between measures) were performed for each ROI. Local BMD and mandibular cross-sectional morphological characteristics were determined by dual x-ray absorptiometry (DXA) and computed tomography (CT). RESULTS: The coefficient of variation for SOS was found to be 1%. SOS measurements at different sites were significantly different. A significant linear relationship was found between SOS and BMD (r2 = 0.62; P < .0001), whereas a nonlinear relationship was found between SOS and Tb.Th/Cort.Th (r2 = 0.53; P < .0001). CONCLUSION: This in vitro study demonstrated the feasibility of SOS measurement through the mandible. Mandibular SOS reflects local BMD and Tb.Th/Cort.Th. In vivo studies are now required to confirm the predictive power of SOS measurement for bone quality assessment and its potential usefulness as a clinical diagnostic technique.

Absorptiometry, Photon↗

PubMed2006

Effects of frequency-dependent attenuation and velocity dispersion on in vitro ultrasound velocity measurements in intact human femur specimens.

Numerous studies have shown that ultrasonic velocity measured in bone provides a good assessment of osteoporotic fracture risk. However, a lack of standardization of signal processing techniques used to compute the speed of sound (SOS) complicates the comparison between data obtained with different commercial devices. In this study, 38 intact femurs were tested using a through-transmission technique and SOS determined using different techniques. The resulting difference in measured SOS was determined as functions of the attenuation and the velocity dispersion. A numerical simulation was used to explain how attenuation and dispersion impact two different SOS measurements (group velocity, velocity based on the first zero crossing of the signal). A new method aimed at compensating for attenuation was devised and led to a significant reduction in the difference between SOS obtained with both signal processing techniques. A comparison between SOS and X-ray density measurements indicated that the best correlation was reached for SOS based on the first zero crossing apparently because it used a marker located in the early part of the signal and was less sensitive to multipath interference. The conclusion is that first zero crossing velocity may be preferred to group velocity for ultrasonic assessment at this potential fracture site.

Aged↗

PubMed2006

Estimation of trabecular thickness using ultrasonic backcatter.

We present a method to estimate trabecular thickness (Tb.Th) in trabecular bones from ultrasound backscatter measurements. The estimation scheme is based on a nonlinear adjustment of predictions from a model to experimental data. The model assumes weak scattering from bone, where scattering is assumed to arise from the elastic solid trabeculae. The fluctuations of acoustical properties between bone tissue and the saturating fluid are assumed to be random and are described by the 3-D spatial autocorrelation function of the medium. In this paper, a Gaussian autocorrelation function is used. The inversion procedure is applied to a set of data measured on 33 femoral bone specimens. Results show that the model can predict both the magnitude and the frequency-dependence of the backscatter coefficient (root mean square error RMSE = 1 dB). The estimated trabecular thickness values are compared to the true trabecular thickness measured on high resolution microcomputed tomography 3-D reconstruction of bones microarchitecture. A close agreement is obtained on average over the group of specimens between predictions and the reference values: true Tb.Th is 132 +/- 12 microm and estimated Tb.Th is 134 +/- 15 microm. However, a moderate correlation between actual and estimated Tb.Th values is found (R2 = 0.44, p<10(-4), RMSE = 8.7 microm) suggesting a modest predictability at the individual level. Sources for the variability of the estimator are studied. Using synthetic rf signals, we demonstrate that the fundamental limitation of the estimator due to speckle noise is approximately 5 microm. Taking into account the measurement errors, the total uncertainty on Tb.Th estimates is of the order of 7 microm. The influence of the attenuation compensation function used to derive the backscatter coefficient is studied. In particular, we demonstrate the necessity of compensating for the effect of the gating time window. The results are discussed with respect to their meaningful clinical value. The requirements to be fulfilled by the performance of the technique change with regard to the question being posed. Two different strategies are examined: 1. characterize trabecular thickness without consideration of bone quantity (or bone mineral density) and 2. estimate trabecular thickness after adjustment for BMD. Considering the first strategy, a comparison between the precision of our estimator and the biological variability leads us to the conclusion that our estimator should only permit to distinguish between micro-architectures characterized by extreme values of trabecular thickness (i.e., very thin or very thick trabecular thickness). In this respect, it would be interesting to test whether the estimator is able to discriminate between rod-like (thin) and plate-like (thick) structures that are known to influence differently bone strength. The second strategy is more demanding in terms of technique performance and our estimator is not able yet to catch small differences in Tb.Th values expected after adjustment to bone density. Progress in the field will require a significant reduction in speckle noise and measurement errors and/or the development of other and more efficient microstructural estimators.

Artifacts↗

PubMed2005

Three-dimensional simulation of ultrasound propagation through trabecular bone structures measured by synchrotron microtomography.

Three-dimensional numerical simulations of ultrasound transmission were performed through 31 trabecular bone samples measured by synchrotron microtomography. The synchrotron microtomography provided high resolution 3D mappings of bone structures, which were used as the input geometry in the simulation software developed in our laboratory. While absorption (i.e. the absorption of ultrasound through dissipative mechanisms) was not taken into account in the algorithm, the simulations reproduced major phenomena observed in real through-transmission experiments in trabecular bone. The simulated attenuation (i.e. the decrease of the transmitted ultrasonic energy) varies linearly with frequency in the MHz frequency range. Both the speed of sound (SOS) and the slope of the normalized frequency-dependent attenuation (nBUA) increase with the bone volume fraction. Twenty-five out of the thirty-one samples exhibited negative velocity dispersion. One sample was rotated to align the main orientation of the trabecular structure with the direction of ultrasonic propagation, leading to the observation of a fast and a slow wave. Coupling numerical simulation with real bone architecture therefore provides a powerful tool to investigate the physics of ultrasound propagation in trabecular structures. As an illustration, comparison between results obtained on bone modelled either as a fluid or a solid structure suggested the major role of mode conversion of the incident acoustic wave to shear waves in bone to explain the large contribution of scattering to the overall attenuation.

Algorithms↗

PubMed2005

Recent developments in trabecular bone characterization using ultrasound.

Currently available quantitative ultrasound technologies to assess cancellous bone are based on the measurements in transmission of speed of sound or slope of frequency-dependent attenuation (so called broadband ultrasonic attenuation). These two parameters are now considered as surrogate markers of site-matched bone mineral density. The ability of ultrasound techniques to provide non-bone mineral density-related bone properties (eg, microstructure) has not been clearly demonstrated yet. This is mainly because of two factors: a lack of understanding of ultrasound propagation with clear identification of the different underlying physical interactions; and the difficulty of performing experiments because of the limited sample size, the large number of statistical relationships to be tested with multiple variables, and the usual strong covariance observed between bone quantity and microarchitecture. The aim of this paper is to review the most recent development in the field of ultrasound characterization of trabecular bone. We present research work on ultrasound backscatter and how it could be used to estimate microarchitectural properties independently of bone quantity, and the first promising results obtained for the estimation of trabecular thickness. We then introduce numeric simulations of wave propagation through trabecular microarchitecture and show how it could contribute to elucidate and better characterize the physical underlying physics and result in more predictive models. These innovative acquisition schemes and the possibility of virtual experiments should altogether contribute to rapid advancement of ultrasonic bone characterization.

Bone Density↗

PubMed2003

Prediction of frequency-dependent ultrasonic backscatter in cancellous bone using statistical weak scattering model.

The goal of this study was to propose a model for the ultrasonic frequency-dependent backscatter coefficient in cancellous bone. This model allows us to address the inverse problem and to predict the mean trabecular thickness. A weak scattering model is used and the backscatter coefficient is expressed in terms of an autocorrelation function of the medium. Different autocorrelation functions (Gaussian, exponential and densely populated media) were used to compute the backscatter coefficient and comparison is made with experimental data for 19 specimens and for frequency ranging from 0.4 to 1.2 MHz. For each specimen, a nonlinear regression was performed and the mean trabecular thickness is estimated. Experimental data and theoretical predictions were averaged over the 19 specimens. A good agreement between experimental data and predictions was found for both the magnitude and the frequency-dependence of the backscatter coefficient. We also found a good agreement between the experimental mean trabecular thickness (Tb. Th = 130 +/- 6.5 micro m) derived from the analysis of bone 3-D microarchitecture using high-resolution microtomography and theoretical predictions (d(Gauss) = 140 +/- 10 micro m, d(exponential) = 153 +/- 12.5 micro m and d(dense) = 138 +/- 6.5 micro m). These results open interesting prospects for the estimation of the mean trabecular thickness from in vivo measurements.

Acoustics↗

PubMed2003

Prediction of backscatter coefficient in trabecular bones using a numerical model of three-dimensional microstructure.

A model of ultrasonic backscattering for cancellous bone saturated by water is proposed. This model assumes that scattering is caused by the solid trabeculae and describes the cancellous bone as a weak scattering medium. The backscatter coefficient is related to the spatial Fourier transform of bone microarchitecture and to the density and compressibility fluctuations between the solid trabeculae and the saturating fluid. The computations of the model make use of three-dimensional numerical images of bone microarchitecture, obtained by tomographic reconstructions with a 10 microm spatial resolution. With this model, the predictions of the frequency dependence and of the magnitude of the backscatter coefficient are reasonably accurate. The theoretical predictions are compared to experimental data obtained on 19 specimens. An accuracy error of approximately 1 dB was found (difference between the averaged experimental values and theoretical predictions). One limit of the model may come from inaccurate values of trabecular bone characteristics needed for the computations (density and longitudinal velocity), which are yet to be precisely determined for human trabecular bone. However, the model is only slightly sensitive to variations of bone material properties. It was found that an accuracy error of 2.2 dB at maximum resulted from inaccurate a priori values of bone material properties. A computation of the elastic mean free path in the medium suggests that multiple scattering plays a minor role in the working frequency bandwidth (0.4-1.2 MHz). It follows from these results that a weak scattering medium model may be appropriate to describe scattering from trabecular bone.

Aged↗