Biomedical subjects

Francesca Monticelli

Publications and source records attributed to Francesca Monticelli.

At least 19 recordsLinked to original sources

PubMed2006

Immediate and 24-hour evaluation of the interfacial strengths of fiber posts.

This study examined the contribution of testing time on the interfacial strengths of fiber posts to radicular dentin. Twenty-five fiber posts were luted to root segments with resin cements and a zinc phosphate cement. Immediately upon setting of the materials, the roots were sectioned into slices and randomly divided into two subgroups, depending on testing time (immediate vs. 24-hours), for push-out test. The results showed an improvement on the interfacial strength after 24 hours of storage. When data were pooled for each cement, the interfacial strengths (MPa) were: Optibond Solo Plus/Nexus: 10.3+/-3.8a; zinc phosphate cement: 10.1+/-2.7a; Multilink: 9.6+/-3.8ab; RelyX Unicem: 7.1+/-4.9bc; AllBond 2/Duolink: 6.7+/-3.5c (groups with same superscript letter were not statistically significant). It is concluded that bond strength can increase during the first 24 hours and that the interfacial strength is predominantly contributed by frictional retention.

Analysis of Variance↗

PubMed2006

Resistance to degradation of resin-dentin bonds using a one-step HEMA-free adhesive.

OBJECTIVE: To evaluate the resistance to degradation of resin-dentin bonds formed with a one-step acetone-containing and HEMA-free adhesive. METHODS: Flat, mid-coronal dentin surfaces were bonded with G Bond under dry and wet conditions. The bonded teeth were subjected to fatigue loading (90 or 250 N) using 5000 or 50000 cycles (at 3.0 Hz) and to chemical degradation (10% NaOCl immersion for 5 h). Specimens were sectioned into beams and tested for microtensile bond strength. Fractographic analysis was performed by scanning electron microscopy (SEM). Additional specimens were processed for morphological examination of the interfaces. RESULTS: Bond strength significantly decreased after the chemical challenge (p<0.05), but not after load cycling. Dentinal moisture did not affect bond strength. Most of the recorded failures were adhesive and located at the top of the hybrid layer. A poorly infiltrated hybrid layer with bubbles entrapped within the adhesive was observed in all groups. CONCLUSIONS: A discrepancy between water/solvent evaporation rate and convective and evaporative water fluxes from the underlying dentin may be responsible of the droplet formation within the adhesive layer, thus reducing bond strength. A "stress relieving" effect may occur during loading. A loss of bonding effectiveness is expected overtime, mainly due to chemical degradation of the hybrid layer.

Acetone↗

PubMed2006

Self-etching adhesives increase collagenolytic activity in radicular dentin.

Endogenous matrix metalloproteinases (MMPs) release from crown dentin and their activation results in degradation of hybrid layers created by dentin adhesives. This study tested the hypothesis that instrumented intraradicular dentin possesses latent collagenolytic activity that is activated by mild self-etching adhesives. Root dentin shavings were produced from 50 cleaned and shaped, saline-irrigated root canals using Gates Glidden drills and rinsed with sodium azide to prevent bacterial growth. Dried dentin powder aliquots were treated with two clinically-relevant MMP inhibitors, 2% chlorhexidine for 10 minutes and 17% EDTA for 1 minute. Additional dentin powder was mixed with Clearfil Liner Bond 2V or Clearfil Tri-S Bond for 1 minute followed by extracting the adhesives with acetone. Dentin powder was also treated with 2% chlorhexidine for 10 minutes before or after adhesive application. Collagenolytic activities of the nine groups were assayed with a fluorometer in 96-well plates, by recording the changes in fluorescence before and after addition of fluorescein-labeled type I collagen. Epoxy resin-embedded powders were examined with TEM for the extent of demineralization. Instrumented, mineralized intraradicular dentin possessed low but detectable collagenolytic activity that was inhibited by chlorhexidine (p < 0.001) and EDTA (p < 0.001). Both adhesives partially demineralized the dentin powder and activated latent MMPs, with 14- to 15-fold increases in collagenolytic activities (p < 0.001) that were significantly (p < 0.001) but incompletely inactivated after 10 min application of chlorhexidine. Mild self-etching adhesives activate latent MMPs without denaturing these enzymes, and may adversely affect the longevity of bonded root canal fillings and posts.

Acid Etching, Dental↗

PubMed2006

Ultrasound effects after post space preparation: An SEM study.

The aim of this study was to evaluate the effect of ultrasonic treatment on occlusion of dentine tubules in root canal walls after post space preparation in endodontically treated teeth. Twenty-four premolars were instrumented and filled using warm vertical condensation; after post space preparation, they were divided into two groups. The control group was treated using the etching procedure. The experiment samples were treated with EDTA irrigation and ultrasound activation for 30 s before the etching procedure. The roots were divided and the canal walls were examined under SEM at 1000x magnification. The debris and open tubule marks were observed at 2, 6, and 10 mm levels using a three-step scale and the differences in marks among the groups were tested for statistical significance. The following were observed: (a) A decrease in debris and open tubule marks in the samples treated with ultrasounds and the control group (p < 0.05), (b) no significant differences between the three levels of post space in debris and open tubule marks in the experiment samples, and (c) significant differences between the apical and coronal levels in debris and open tubule marks in the control group.

Dental Etching↗

PubMed2006

Improving the quality of the quartz fiber postcore bond using sodium ethoxide etching and combined silane/adhesive coupling.

The influence of different silane/dentin adhesives on the microtensile bond strengths of a hybrid composite to sodium ethoxide-etched quartz fiber posts was investigated. A prehydrolyzed silane was applied without an adhesive and compared to two-component systems in which hydrolysis of the silane occurred after mixing with the acidic monomer present in the dentin adhesives. Nanoleakage along post/core interfaces was examined after silver nitrate tracer penetration using scanning electron microscopy. Higher interfacial strengths and more uniform adaptation between the etched fiber post surface and the composite core were recorded after post treatment with silane/adhesive couplings. The combination of silane with two-step self-etch adhesives improves the chemical retention of composites around etched fiber posts with minimal nanoleakage. Although the one-step self-etching adhesive/silane combination enhances post adhesion, the extensive nanoleakage provides channels for rapid water sorption that may expedite hydrolytic degradation of the post/core interface.

Composite Resins↗

PubMed2006

Bond strength performance of different resin composites used as core materials around fiber posts.

OBJECTIVES: To evaluate the microtensile bond strengths of different resin composites used as core materials around fiber posts. METHODS: Forty DT Light-Posts (RTD) were randomly divided into eight groups, according to the resin composite used. They included two core materials specifically developed for core build-up--Group 1: Core-Flo (Bisco Inc.) and Group 2: UniFil Core (GC Corp.); three hybrid composites--Group 3: Tetric Ceram (Ivoclar-Vivadent), Group 4: Gradia Direct (GC Corp.), Group 5: Bisfil 2B (Bisco, Inc.); and three flowable composites--Group 6: AEliteflo (Bisco, Inc.), Group 7: Filtek Flow (3M ESPE) and Group 8: UniFil Flow (GC Corp). A cylindrical plastic matrix was placed around the silanized post and filled with the respective resin composite. Each bonded post provided five to eight sticks for microtensile testing. Each stick was loaded to failure under tension at a cross-head speed of 0.5mm/min. One-way ANOVA and Tukey's test were used for statistical analysis. Scanning electron microscopy (SEM) was used to evaluate the interface of the fractured sticks. RESULTS: Resin composites exhibited a significant influence on microtensile bond strength (p<0.05). Core-Flo showed the highest bond strength (11.00+/-0.69 MPa) although it was not statistically significantly different from all groups, except from the flowable composites. Under SEM, all the composites adapted well to the fiber post, with a variable extent of voids observed along the fractured composite interfaces. SIGNIFICANCE: Although good adaptation to the post surface was achieved, bond strength to fiber post remains relatively weak. Core build-up and hybrid composites are better alternatives to flowable composites as core build-up materials.

Barium Compounds↗

PubMed2006

Effects of adhesive systems and luting agents on bonding of fiber posts to root canal dentin.

The study evaluated the influence of different luting materials on the microtensile bond strength of glass fiber posts to root canal dentin. Thirty extracted maxillary premolars were endodontically treated, and the roots were prepared for post cementation using the FRC Postec system (Vivadent). Two luting materials (Multilink, Vivadent and Clearfil Photo Core, Kuraray) were used in combination with three adhesive: Multilink Primer (Vivadent), Clearfil Photo Bond, and Clearfil New Bond (Kuraray). A composite build-up was performed around the root to provide adequate gripping during testing. Specimens were cut to obtain beams with the post in the center and with the radicular dentin overlaid by the composite build-up on each side. Microtensile testing was performed with a universal testing machine at a cross-head speed of 0.5 mm/min. The failure mode was classified under a stereomicroscope and four representative beams of each group were selected for SEM analysis. Bond strength data that were analyzed with two-way ANOVA and Student-Newman-Keuls multiple comparisons tests revealed that adhesive systems, luting materials, and the interaction between these two factors significantly influenced the bond strength results (p < 0.01). Multilink applied with its own adhesive system obtained the best results, while the lowest bond strength was achieved with clearfil photo core in combination with multilink primer.

Adhesives↗

PubMed2006

A novel method to obtain microtensile specimens minimizing cut flaws.

Cutting procedures conventionally used for microtensile specimens' preparation induce mechanical stress and attrition, leading to cracks in dental structure. Enamel is mainly affected due to its high module of elasticity and brittleness. As an alternative to the conventional diamond saw, a diamond wire was used for cutting. The two techniques were compared in efficacy in the preparation of two different sample shapes. Forty human molars, flattened in dentin or enamel, were used for sample preparation. After bonding procedures, resin block was built incrementally. Half of the teeth were cut using a diamond disc and the other half using a diamond wire, obtaining slabs and stick-shaped specimens. Slabs were then shaped to obtain hourglass samples. Five samples from each subgroup were randomly selected for Scanning electron microscopy (SEM) analysis. Remaining samples underwent microtensile test and the results were analyzed, including/excluding premature failures (pf), with three-way ANOVA and Tukey test, performed separately, by substrate. On dentin, only the exclusion of pf showed a significant higher bond strength values than when pf were included (p < 0.05); however, on enamel, all factors (equipment, shape and including/excluding pf) had a significant effect on bond strength (p<0.05). The most remarkable finding from SEM was the incidence of specimens' cracks and defects when diamond blade was used. Scratches and cracks in the hourglass-shaped specimens were also detected. In both cases, enamel was more affected than dentin.

Dental Enamel↗

PubMed2006

A simple etching technique for improving the retention of fiber posts to resin composites.

Coupling of fiber posts to composites is hampered by absence of chemical union between epoxy resins and methacrylate-based resins. This study examined a clinically feasible protocol for creating micromechanical retention on the surface of fiber posts, using hydrogen peroxide etching to remove the surface layer of epoxy resin. This was followed by silanization of the exposed quartz fibers to enhance their chemical bonding to composites. Etching with 24% H2O2 for 10 min or 10% H2O2 for 20 min produced a 50 microm thick surface zone that is depleted of epoxy resin, leaving intact, undamaged quartz fibers for silanization. Low viscosity flowable composites were employed to infiltrate this zone, to simulate the creation of hybrid layers in acid-etched dentin by dentin adhesives. Interfacial strengths were enhanced with the adjunctive use of H2O2 etching and silanization, and were probably dependent on the ability of the flowable composites to completely infiltrate this interdiffusion zone.

Barium Compounds↗

PubMed2006

Effect of dentin adhesives used as sealers and provisional cementation on bond strength of a resin cement to dentin.

PURPOSE: To evaluate the effects of dentin adhesives employed as resin sealers and provisional cementation on the bond strengths of a resin cement to dentin. METHODS: A two-step etch-and-rinse adhesive (Excite DSC--Group 1) and two-step self-etch adhesive (AdheSE--Group 2) were applied to exposed dentin surfaces prepared from human molars (N=4). Water was used instead of a resin sealer in control Groups 3 and 4. A eugenol-free provisional cement (except for Group 4) was applied to the treated surfaces. After storing in distilled water for 1 week, the provisional cement was removed and cylindrical composite blocks were luted with a resin cement (Variolink II). 0.9 x 0.9 mm sticks were produced from these luted specimens for microtensile bond testing and SEM examination. RESULTS: One-way ANOVA revealed that neither the resin sealer nor the temporary eugenol-free cement had a negative effect on the final bond strength (P> 0.05). Mixed failures were predominantly identified from SEM.

Acrylic Resins↗

PubMed2006

An investigation of the interfacial strengths of methacrylate resin-based glass fiber post-core buildups.

PURPOSE: To assess the microtensile bond strengths of a core composite with two different viscosities, MultiCore Flow (F) and Heavy Body (HB) (Ivoclar-Vivadent), on dentin and glass-fiber posts with methacrylate resin matrices, in combination with three dentin adhesives: Syntac Classic (S), Excite DSC (E), and AdheSE (A) (Ivoclar-Vivadent). The omission of an intermediate adhesive coating on silanized posts was also examined to see if this resulted in post-core bond strength reduction. MATERIALS AND METHODS: Six experimental dentin and 6 experimental post groups were formed with different core material viscosity/adhesive combinations. In two control post groups, no adhesive was applied on the silanized posts. The core material was stratified on deep dentin or on the posts. From the built-up teeth and the post-core units, microtensile sticks were obtained. RESULTS: The bond strengths measured on dentin were (MPa): S-F 12.7 +/- 6.4; S-HB 18.3 +/- 7.6; E-F 33.1 +/- 13.7; E-HB 34.1 +/- 10.2; A-F 33.5 +/- 14.8; A-HB 35.5 +/- 14.4. The post-core strengths were: S-F 9.4 +/- 2.5; S-HB 7.1 +/- 3.8; E-F 8.6 +/- 3.8; E-HB 8.1 +/- 4.4; A-F 8.1 +/- 2.7; A-HB 8.3 +/- 2.4; no adhesive-F 10.4 +/- 3.6; no adhesive-HB 6.3 +/- 2.7. Significantly higher interfacial strengths were achieved on dentin than on posts (p < 0.05). Adhesive type significantly affected core-dentin adhesion, with Excite DSC and AdheSE outperforming Syntac Classic (p < 0.05). Neither core material viscosity nor adhesive type significantly influenced post-core adhesion (p > 0.05). Omission of an intermediate adhesive layer had no effect on post-core adhesion (p > 0.05). CONCLUSIONS: Even after post surface silanization, the bond established by the composite core material with the post remains weaker than that created with coronal dentin.

Acrylic Resins↗

PubMed2005

Effect of temperature on the silane coupling agents when bonding core resin to quartz fiber posts.

OBJECTIVES: To evaluate the effect of different silane agents and air-drying temperatures on bond strength of translucent quartz fibre posts to composite resin. METHODS: The post surface was etched with 10 vol% hydrogen peroxide for 20 min. A two-liquid coupling agent containing 4-methacryolxyethyl trimellitate anhydride (4-META) and gamma-trimethoxysilyil propyl methacrylate (gamma-MPTS) and two pre-hydrolyzed single component silanes containing 3-methacryloxypropyltrimethoxysilane (3-MPS) and glycid-oxi-propyl-trimetil-oxi-silane (GPS), respectively, were used for treating the fiber posts. Two different post-silanization drying temperatures were applied (21 and 38 degrees C). A dual-cure composite resin (Core Paste XP) was selected to build-up the core around posts, obtaining cylindrical specimens that were serially cut in beams and subsequently loaded in tension (muTBS) at a cross-head speed of 1 mm/min until failure. Bond strength data were statistically analyzed by two-way ANOVA and Student-Newman-Keuls tests (alpha=0.05). RESULTS: Warm air-drying determined significantly higher bond strengths (p<0.001) for glycid-oxi-propyl-trimetil-oxi-silane (11.6 MPa) and 4-methacryolxyethyl trimellitate anhydride/gamma-trimethoxysilyil propyl methacrylate silane (11.7 MPa). These two systems exhibited lower bond strengths (6.9 and 8.8 MPa, respectively) than 3-methacryloxypropyltrimethoxysilane (11.0 MPa) when dried at 21 degrees C. No statistical differences were recorded for 3-methacryloxypropyltrimethoxysilane when drying at 21 or 38 degrees C. SIGNIFICANCE: The composition of the silane coupling agent in terms of acidic content, solvent rate or degree of hydrolysis may influence resin/post bond strength when dried at 21 degrees C. Drying at 38 degrees C most likely facilitates the evaporation of solvents present in the silane agent, resulting in increased bond strength of the composite resin to the fiber post.

Acid Etching, Dental↗

PubMed2005

Post-surface conditioning improves interfacial adhesion in post/core restorations.

OBJECTIVES: To verify the influence of different etching procedures of the post-surface on microtensile bond strength values between fiber posts and composite core materials. METHODS: 60 DT Light Posts were divided into 10 subgroups using five different chemical surface treatments and two composite materials to build-up the abutment. Chemical surface treatments including etching with potassium permanganate; treatment with 10% hydrogen peroxide; treatment with 21% sodium ethoxide; etching with potassium permanganate and 10 vol.% HCl; silanization (control group) were performed on the post's surface. The build-up was performed using (A) Core Paste XP (Dent Mat) and (B) Unifil Flow (GC). Two samples of each group were randomly selected to investigate the morphologic aspect of the post/core interface with a scanning electron microscope (SEM). The remaining specimens were cut so as to obtain microtensile sticks that were loaded in tension at a cross-head speed of 1mm/min until failure. The statistical analysis was performed using two-way ANOVA and the Tukey's test for post-hoc comparisons (alpha=0.05). RESULTS: SEM examination showed an interpenetrating adhesion network between the treated fiber post-surface and the composite material in all the groups tested. The results achieved with potassium permanganate had a significant influence on microtensile interfacial bond strength values with both the tested materials. Post-superficial treatments enhanced the bond strength particularly of Core Paste XP. SIGNIFICANCE: Etching procedures showed a similar effect on the post-surface and enhanced the adhesion of composite core build-ups as a result of micromechanical and chemical retention.

Composite Resins↗

PubMed2005

The influence of the cutting speed on bond strength and integrity of microtensile specimens.

OBJECTIVES: To verify the influence of cutting speed during microtensile specimen preparation, on bond strength values and on the sample microscopic integrity of a single-bottle adhesive system to enamel and dentin. METHODS: Thirty sound human third molars were restored with light-cured Excite and Tetric Ceram according to manufacturers' instructions, being half in ground enamel [E], and half in flat dentin [D]. After 24 h storage in distilled water at 37 degrees C, the restored teeth were sectioned in x and y-axes under different cutting speed: 100, 200 or 400 RPM, obtaining stick shaped specimens with cross-sectional area of 1.0 mm(2). Five specimens from each experimental group were randomly selected before being loaded for SEM analysis, while the remaining samples were subjected to the microtensile bond strength test. Because the variance of dentin specimens was significantly higher than the variance of enamel samples, analysis of the influence of the cutting speed was performed separately, by substrate. RESULTS: The mean values in MPa were calculated including/excluding premature failures: E1: 24.67/27.31(a); E2: 24.03/25.81(a); E4: 19.06/21.52(b); D1: 35.80/36.33(A); D2: 35.84/36.65(A); D4: 37.78/38.50(A). In the SEM analysis, better integrity of the dentin specimens was observed when compared to the enamel samples, for which the integrity was greater in the lower cutting speed groups. SIGNIFICANCE: It can be concluded that the cutting speed is an important factor that should be considered, mainly when enamel is involved, since it may affect the bond strength results and the integrity of the specimens.

Acid Etching, Dental↗

PubMed2005

Fatigue resistance and structural characteristics of fiber posts: three-point bending test and SEM evaluation.

OBJECTIVES: The aim of the present study was to assess the fatigue resistance of different types of fiber posts, and to observe their ultrastructure through scanning electron microscopy (SEM) before and after undergoing the fatigue test. METHODS: Eight types of fiber posts were selected for this study. Easypost (Group 1), Para Post Fiber White (Group 2), FibreKor (Group 3), Ghimas White (Group 4), DT Light-Post radiopaque (Group 5), FRC Postec (Group 6), Luscent Anchors (Group 7), Snowpost (Group 8). Ten out of fifteen posts in each group were used for the fatigue test, and the other five were processed for SEM. A three-point bending machine, loading at an angle of 90 degrees and a frequency of 3 Hz was employed for fatigue testing. The test was carried out until two million cycles were completed or until fracturing of the post. SEM evaluation was performed using a three-step scale method to assess the fiber/resin ratio and fiber dimension. RESULTS: The fatigue test showed statistically significant differences among the different posts. Groups 5 and 6 performed better than all the other groups, withstanding practically all the stipulated number of load cycles. All the other posts fractured before the end of the test. SEM observations of the surface integrity of the fiber posts showed that Groups 4-6 obtained better scores for both longitudinal sections and cross-sections. SIGNIFICANCE: There was a large variation in the response of different kinds of fiber posts a fatigue resistance test. The absence of correlation between results of fatigue testing and parameters of structural integrity suggests that the latter mostly reflect random variations in the fiber post manufacturing process.

Composite Resins↗

PubMed2005

The adhesion between prefabricated FRC posts and composite resin cores: microtensile bond strength with and without post-silanization.

OBJECTIVES: Aim of the study was to measure the adhesion between two types of translucent prefabricated FRC posts (FRC Postec, Ivoclar-Vivadent, FRC; Light-Post, RTD, LP), and two types of flowable composites used as core materials (UnifilFlow, GC, UF; Tetric Flow, Ivoclar-Vivadent, TF), with or without the application of a silane (Monobond-S, Ivoclar-Vivadent, S) on the post surface. METHODS: The experimental groups were: 1.1 FRC+UF; 1.2 FRC+S+UF; 1.3 FRC+TF; 1.4 FRC+S+TF: 2.1 LP+UF; 2.2 LP+S+UF; 2.3 LP+TF; 1.4 LP+S+TF. The bond strength at the interface between post and core was measured with the microtensile non-trimming technique. Thirty to thirty-five beam-shaped specimens per group were obtained from cylinders of core material, which had been built up around the post by progressively adding small increments of composite resin. Each specimen was loaded in tension until failure at either one of the two post-core interfaces present in each stick. The differences in interfacial bond strength among the groups were tested for statistical significance with the two-way ANOVA. RESULTS: The measured bond strengths in MPa were: [table: see text]. The statistical analysis revealed that post-silanization had a significant effect on adhesion (p<0.05). With any combination of post and core materials tested, the application of a silane onto the post surface prior to building up the core significantly increased the post-core bond strength. SIGNIFICANCE: For improved adhesion at the interface between prefabricated FRC posts and composite resin cores, post-silanization is advisable.

Adhesiveness↗

PubMed2005

Effectiveness of resin-coated gutta-percha cones and a dual-cured, hydrophilic methacrylate resin-based sealer in obturating root canals.

The introduction of a polybutadiene-diisocyanate-methacylate resin-coating of gutta-percha enables the polyisoprene to be chemically coupled to methacrylate-based resin root canal sealers. This study examined the effectiveness of using passively fitting cones of this type of gutta-percha with a dual-cured version of EndoREZ sealer in obturating cleaned and shaped root canals. The hydrophilic nature of the sealer enabled the creation of an extensive network of 800 to 1200 mum long sealer resin tags after removal of the endodontic smear layer. Although no adhesive was employed, thin hybrid layers in root dentin were observed when EDTA was used as the final rinse. Nevertheless, interfacial gaps and silver leakage could be observed along the sealer-dentin interfaces that might be attributed predominantly to polymerization shrinkage of the sealer. Gaps and silver leakage were also identified between the gutta-percha resin-coating and the sealer.

Bicuspid↗

PubMed2005

Scanning electron microscopic evaluation of fiber post-resin core units built up with different resin composites.

PURPOSE: To microscopically evaluate the structural characteristics of post-and-core units made of a fiber post and of different types of resin-based composites used as core materials. METHODS: 55 endodontically treated human maxillary incisors were prepared for receiving a fiber post (AEsthetic Post Plus). One-Step and C&B resin cement were used for luting the post. Samples were randomly divided into 11 groups. Eleven different composite materials were selected to build-up the abutment. The materials on trial were: Group 1: Z100; Group 2: Light-Core; Group 3: Lumiglass; Group 4: Gradia Direct; Group 5: Build-It!; Group 6: Tetric Ceram; Group 7: Biscore self-curing; Group 8: Unifil Flow; Group 9: Definite Flow; Group 10: Tetric Flow; Group 11: AEliteFlo. All the post-and-core specimens were cut perpendicularly to their long axis and processed for SEM observations. The presence of voids/bubbles within the resin abutment and of defects at the interface between the post surface and the core material was scored. These aspects were quantified with reference to a predefined index. The differences among the scores were tested for statistical significance (P< 0.05). RESULTS: Cores built up with flowable composites showed the highest integrity and the best adaptation onto the post.

Analysis of Variance↗