Biomedical subjects

Frank Dubois

Publications and source records attributed to Frank Dubois.

5 recordsLinked to original sources

PubMed2006

Digital holographic microscopy with reduced spatial coherence for three-dimensional particle flow analysis.

We investigate the use of a digital holographic microscope working in partially coherent illumination to study in three dimensions a micrometer-size particle flow. The phenomenon under investigation rapidly varies in such a way that it is necessary to record, for every camera frame, the complete holographic information for further processing. For this purpose, we implement the Fourier-transform method for optical amplitude extraction. The suspension of particles is flowing in a split-flow lateral-transport thin separation cell that is usually used to separate the species by their sizes. Details of the optical implementation are provided. Examples of reconstructed images of different particle sizes are shown, and a particle-velocity measurement technique that is based on the blurred holographic image is exploited.

Journal Article↗

PubMed2004

Partial spatial coherence effects in digital holographic microscopy with a laser source.

We investigate a digital holographic microscope that permits us to modify the spatial coherence state of the sample illumination by changing the spot size of a laser beam on a rotating ground glass. Out-of-focus planes are refocused by digital holographic reconstruction with numerical implementation of the Kirchhoff-Fresnel integral. The partial coherence nature of the illumination reduces the coherent artifact noise with respect to fully coherent illumination. The investigated configuration allows the spatial coherence state to be changed without modifying the illumination level of the sample. The effect of the coherence state on the digital holographic reconstruction is theoretically and experimentally evaluated. We also show how multiple reflection interferences are limited by the use of reduced spatial coherent illumination.

Journal Article↗

PubMed2003

Phase-shifting schlieren: high-resolution quantitative schlieren that uses the phase-shifting technique principle.

A quantitative autocalibrated high-resolution schlieren technique for quantitative measurement of reflective surface shape is proposed. It combines the schlieren principle with the phase-shifting technique that is generally used in interferometry. With an appropriate schlieren filter and appropriately tailored setup, some schlieren fringes are generated. After application of the phase-shift technique, the schlieren phase is calculated and converted into beam deviation values. Theoretical and experimental demonstrations are given. The technique is validated on a reference target, and then its application in a fluid physics experiment is demonstrated. These two examples show the potential of the phase-shifting schlieren technique that in some situations can become competitive with interferometry but with a much better dynamic range and with variable sensitivity. The technique can also be used to measure refractive-index gradients in transparent media.

Journal Article↗

PubMed2002

Reduction of the correlation sensitivity to the changes of the input illumination by a post processing based on the correlation statistics.

Linear-correlation amplitude changes when the intensity level of the input image is modified. As recognition is often based on the correlation-peak level, a change of the input illumination may result in a false recognition. We propose an illumination-change compensation by a post processing of the correlation distribution that is based on statistical measures of the correlation histograms. The theoretical background and simulation results are provided in the frame of an actual application in biology.

Journal Article↗

PubMedDate not supplied

Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration.

Cancer cell motility and invasion are critical targets for anticancer therapeutics. Whereas in vitro models could be designed for rapid screening with a view to investigate these targets, careful consideration must be given to the construction of appropriate model systems. Most investigations focus on two-dimensional (2-D) assays despite the fact that increasing evidence suggests that migration across rigid and planar substrates fails to recapitulate in vivo behavior. In contrast, few systems enable three-dimensional (3-D) cell migration to be quantitatively analyzed. We previously developed a digital holographic microscope (DHM) working in transmission with a partially spatial coherence source. This configuration avoids the noise artifacts of laser illumination and makes possible the direct recording of information on the 3-D structure of samples consisting of multiple objects embedded in scattering media, such as cell cultures in matrix gels. The software driving our DHM system is equipped with a time-lapse ability that enables the 3-D trajectories of living cells to be reconstituted and quantitatively analyzed.

Cell Count↗