Biomedical subjects

Fred E Lytle

Publications and source records attributed to Fred E Lytle.

2 recordsLinked to original sources

PubMed2002

Performance of submillimeter square hollow waveguides.

Propagation losses are determined for 100 microm x 100 microm square, hollow waveguides constructed from glass capillaries. The small size makes it possible to observe optical effects not easily seen with larger waveguides. The depletion of higher-order even modes creates a large, nonlinear loss. Over a distance of a meter the loss approaches the smaller, linear value expected for the fundamental mode. Additionally, the lowest two even modes beat to produce an oscillatory loss with a period of approximately 2 cm. Making the focal radius 0.35 the waveguide width minimizes these two effects. In a related study, 50-microm waveguides embossed in polydimethylsiloxane are shown to have losses similar to glass capillaries.

Models, Theoretical↗

PubMed2002

On-chip absorption measurements using an integrated waveguide.

Square hollow waveguides are used to integrate measurement of absorption with chip-based electrophoresis. The 50x50 microm liquid channel and 50x50 microm waveguide are etched as a negative pattern into a silicon master and replicated as a positive in poly-dimethylsiloxane (PDMS). The uniform refractive index of the chip prevents guiding by total internal reflection. Instead, light at 488 nm is guided by reflection at the air-PDMS interface. The waveguide has a 60% efficiency over a distance of 3.2 cm. Separation of fluorescein and the dye BODIPY is demonstrated. A detection limit (S/N=3) of 200 microM fluorescein is obtained using a 50 microm pathlength and a simple photocell detector.

Absorption↗