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Biomedical subjects

Fumiyuki Karakama

Publications and source records attributed to Fumiyuki Karakama.

2 recordsLinked to original sources

Does a clinical evaluation of oral cleanliness correlate with caries incidence in preschool children? Findings from a cohort study.

The aim of this study was to assess whether a clinical evaluation of oral cleanliness reflects subsequent caries incidence. Oral examination of 180 children (1- to 4-year-olds) was carried out twice in a six-month period. Caries prevalence at baseline (dfs) in 1- to 2-year-olds (group A) and 3- to 4-year-olds (group B) correlated significantly with oral cleanliness as well as salivary mutans streptococci count (MS). Caries increment (delta(dfs)) correlated significantly with oral cleanliness in group A but not in group B, while delta(dfs) significantly correlated with MS in group B and slightly correlated with that in group A. ANCOVA revealed that dfs was significantly higher at the second examination than at baseline in group B, even after adjusting for oral cleanliness. This finding was confirmed by Wilcoxon test when group B was divided in three categories (low, middle and high) based on oral cleanliness. This suggests that the relationship between delta(dfs) and oral cleanliness decreased with age and that the significant positive correlation found in group B by our point-prevalence survey is derived from the remainder of the positive correlation that occurred at a younger age.

Age Factors↗

An improved method for detecting mutans streptococci using a commercial kit.

The improved detection of mutans streptococci (MS) in individuals was investigated using several modifications to a commercially available kit, Dentocult SM. Significantly better detection of MS was achieved using plaque from the four approximal surfaces at two interdental spaces than with saliva (P < 0.001). Furthermore, the MS estimates for approximal surfaces at the same interdental space were similar (kappa = 0.654) suggesting that differentiating the two surfaces does not improve the detection of MS, and that increasing the number of interdental spaces sampled is a more effective option. This study also evaluated a modification to the standard Dentocult SM site strip method in which two strips were incubated per broth vial so that plaque from eight interdental spaces could be tested at the same time (new method). The results were compared to those obtained when one strip was incubated per broth vial (standard method). Although the MS estimates by the new and standard methods were comparable (kappa = 0.721), the efficiency of MS detection was improved significantly by increasing the number of sites used for MS estimates (P = 0.01). In conclusion, MS detection at eight interdental spaces is recommended using the new Dentocult SM method.

Adult↗