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Biomedical subjects

G A Berkhoff

Publications and source records attributed to G A Berkhoff.

4 recordsLinked to original sources
PubMed1977

Isolation and identification of anaerobes in the veterinary diagnostic laboratory.

In a 1-year survey (1975 to 1976) of anaerobic bacteria recovered from diseased animals, anaerobes were as follows: Clostridium spp, 50%; gram-positive nonspore-forming anaerobic bacilli, 19%; gram-negative anaerobic bacilli, 19%; Actinomyces spp, 10%; and anaerobic cocci, 1%. Anaerobes were in approximately 61% of the specimens that were culturally positive for any bacteria. Approximately 25% of the specimens did not yield any bacteria (sterile specimens). The method for isolating and identifying anaerobes was based upon the use of reducible solid mediums and was specially designed for veterinary diagnostic laboratories. Emphasis was placed on the selection, collection, and transportation of specimens.

Anaerobiosis↗
PubMed1975

Ulcerative enteritis--clostridial antigens.

The causative agent of ulcerative enteritis (UE) is a clostridium and it is believed that it belongs to a new, hitherto undescribed species. It has been named, tentatively, Clostridium colinum. Transmission experiments carried out with artificial cultures of this clostridium have clarified some aspects of the pathogenesis of UE and have brought into focus other aspects of its pathogenesis, epizootiology, and immunology that need to be investigated. Support for research is needed: 1) To investigate the distribution in nature of C colinum. For this purpose the development of more suitable selective and differential culture mediums should be encouraged. 2) To further study the pathogenesis of UE through application of the fluorescent labeled antibody procedure. 3) To search for toxins produced by this clostridium, possibly through use of ligated intestinal loops in chickens. 4) To develop a quick and simple means for the differential diagnosis of UE. Use of a fluorescent antibody test has been suggested as an area of research to fill this need.

Animals↗
PubMedDate not supplied

Fluorescent antibody test in diagnosis of ulcerative enteritis.

A direct fluorescent antibody (FA) test to demonstrate the presence of Clostridium colinum in cryostat sections of liver and intestine from chickens with ulcerative enteritis ("quail disease") was investigated. Both field cases and experimentally infected birds were studied by FA, bacterial isolation of the clostridium, and histopathology. The FA test proved highly specific for Cl. colinum. It was concluded that the described direct FA procedure offers simplicity and speed over bacterial isolation procedures for etiologic diagnosis of ulcerative enteritis.

Animals↗