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Biomedical subjects

G A Ermolin

Publications and source records attributed to G A Ermolin.

At least 73 records · Page 4Linked to original sources
PubMed1984

[Fibrinolytic action of an enzyme preparation conjugated with specific antibodies].

The authors have modified alpha-chymotrypsin covalently bound via aldehyde dextran with polyclonal antibodies to fibrinogen. Modification does not lead to any substantial changes in the catalytic properties of the enzymes or to appreciable losses in specific activity of antibodies. At the same time in experimental thrombolysis in vitro, the derivatives obtained exhibit a higher fibrinolytic action as compared with control preparations. It is assumed that the effect observed is accounted for by an increase in tropism of the conjugate obtained to the fibrin clot.

Antibodies↗
PubMed1984

[Changes in fibronectin concentration during therapeutic plasmapheresis. Therapeutic effectiveness of selective removal of fibronectin in immune complex pathology].

Variation in the concentration of plasma fibronectin (FN) seen during 65 sessions of therapeutic plasmapheresis was studied in 20 inpatients with the following diagnoses: acute leukemias (4), paraproteinemic hemoblastoses (5), Sjögren's syndrome (2), protracted septic endocarditis (1), systemic lupus erythematosus (17), acute polyradiculoneuritis (1), multiple sclerosis (3). The patients' age ranged within 19 to 64 years. There were 10 men and 10 women. The concentration of FN in 218 plasma samples was measured by the ELISA. It was discovered that plasma of healthy donors contained 200 to 400 micrograms/ml FN (M +/- 1.5 sigma). Prior to plasmapheresis the glycoprotein content in all the patients was on the average within normal (300 micrograms/ml). After the session the concentration of FN decreased almost two-fold (130 micrograms/ml); after 6 hours it was 175 micrograms/ml on the average, whereas after 24-48 hours it reached the initial level. Repeated plasmapheresis sessions carried out once every 3-4 days did not lead to the depletion of the FN pool, since its level reached the initial one over the first 24-48 hours. Such a mechanism was unchanged regardless of the initial level of FN recorded in the patients blood (high, normal or low). No relationship was found between FN deficiency which developed after plasmapheresis and infectious complications. It is assumed that FN deficiency per se cannot be responsible for the patients' decreased resistance to infection. Three patients received a series of selective plasmapheresis. Sjögren's syndrome and immune complex vasculitis was diagnosed in one female patient, hemorrhagic vasculitis due to chronic hepatitis in another female patient, the third female patient manifested gammapathy with cryoglobulinemia.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Immunological methods of myoglobulin determination in the serum for diagnosing myocardial infarct].

The blood serum myoglobin content was measured in 22 patients with myocardial infarction using ELISA and RIA. High diagnostic significance of both methods is shown. High linear relationship was observed between the data obtained by both methods for estimating myoglobin which allows the conclusion that the shapes of the myoglobin concentration curves are very similar. Taking into account some advantages of ELISA as compared to RIA one may suppose that it will be of great importance in the diagnosis of myocardial infarction at the hospital stage.

Adult↗
PubMed1984

[Immunoenzyme method of diagnosing suppurative-septic complications due to Pseudomonas aeruginosa and Proteus].

Two test systems, based on the inhibition of the reaction of enzyme-labeled antibodies and on the indirect reaction of enzyme-labeled antibodies, have been developed with a view to diagnosing purulent and septic complications caused by P. aeruginosa and Proteus in accordance with the results of the determination of the corresponding microbial antigens and antibodies to these antigens in the patients' blood. An increase in the levels of P. aeruginosa and Proteus antigens during the first 3 days of the development of complications and in the titers of antibodies on days 7-10 indicates that the purulent septic complication is caused by the given infective agent. A high correlation of the results obtained by bacteriological and serological diagnostic methods has been observed.

Antibodies, Bacterial↗
PubMed1984

[Blood fibronectin level in experimental burn shock].

The blood fibronectin concentration in rats was measured by the ELISA during burn shock. Fifteen minutes after burn the fibronectin level was found to drop by 30% of the initial level on the average. Deep hypofibronectinemis lasted up to 24 h after burn. At the same time the clinical and pathomorphological picture of the shock was fairly pronounced. The survived animals demonstrated a progressive normalization of the fibronectin level. The fibronectin level completely returned to normal by the outcome of the second day after burn and then progressed to reactive hyperfibronectinemia by 70-72 h. The data obtained point to the involvement of fibronectin into the pathogenesis of burn shock, which is likely to be connected with the mechanism of reticuloendothelial system dysfunction.

Animals↗
PubMed1984

[Fibronectin level in coronary sinus blood in myocardial ischemia caused by atrial stimulation].

Patients with coronary heart disease and angina pectoris were examined. The blood outflowing from the myocardium at the height of ischemia induced by atrial stimulation was found to have lower levels of fibronectin. Ten minutes after the stimulation discontinuation the concentration of fibronectin returned to baseline. A reduction in the fibronectin content during myocardial ischemia seems to be related to its consumption as a result of maintaining antithrombotic function.

Adult↗
PubMed1983

[Pulmonary artery thromboembolism: its clinical and coagulative diagnosis].

A total of 250 subjects were investigated using a diagnostic search flow-chart. Pulmonary artery thromboembolism (PATE) was diagnosed in 102 patients. The results have demonstrated the value of laboratory tests employed--markers of thrombin- and plasminemia and platelet activation--for PATE diagnosis. To diagnose PATE, levels of fibrinogen-fibrin and beta-thromboglobulin degradation products should be measured in myocardial infarction patients, and plasma soluble fibrin measurement should be added to those in patients with circulatory insufficiency. In recurrent PATE, continuous increase of plasma beta-thromboglobulin and soluble fibrin levels, antithrombin III exhaustion and reduced levels of fibrinogen-fibrin degradation products are of diagnostic value.

Antithrombins↗
PubMed1983

[Diagnostic test-system for the immunoenzymatic detection of meningococcal antigen].

To detect meningococcal antigen, the use of the enzyme-labeled immunosorbent assay (ELISA), a new variant of the immunoenzymatic method, permitting one to carry out quantitative analysis, is proposed. The optimum conditions for the test to detect group A meningococcal antigen, as well as the procedure for the approbation of the test on patients with meningococcal infection and on healthy persons, have been worked out. The method is shown to be highly specific and sensitive.

Animals↗
PubMed1981

[Parameters for constructing a test system for the isoenzymatic detection of meningococcal antibodies].

The optimum conditions for the detection of specific humoral antibodies in patients with meningococcal infection by the immunoenzymatic method have been determined: the sensitizing dose of the antigen is 20 microgram of protein per 1 ml for group A meningococcal antigen and 25 microgram of protein per 1 ml for group C meningococcal antigen, the pH of the buffer solutions is 5.0-6.0 for group A meningococcal antigen and 12.0 for group C meningococcal antigen, the time and temperature of incubation should be 18-20 hours at 14 degrees C. The diagnostic test system developed in this research is capable of detecting antibodies with the accuracy of several ng.

Antibodies, Bacterial↗